Abstract:

This experiment based on the established multiple cotyledons and flower-like shape (MCFS) repetitive embryogenesis system in Camellia nitidissima Chi.. The difference in the total proteins expression of the materials during the MCFS pathway and normal developmental pathway was analyzed by 2-DE. The results showed that: along with the growth advancement of somatic embryo, the protein variety and quantity increased first, and reached to the most in the cotyledon embryo stage, afterward gradually weakened, even vanished, and became to least in the seedling stage; the differences were distinct and existed both in every single phase of the same pathway and in the same phases of the two different pathways, PD(14.9kD) was specific protein in the MCFS pathway, that expressed in the calli which can differentiate MCFS embryo, as well as every stage from the sphere embryo to mature embryo.