Abstract:

In order to study the relationship between E2 and MHC at different time point, the E2 gene of Chinese classical swine fever virus (CSFV) Shimen strain was cloned, then inserted into the expression vector pcDNA 3.0, eukaryotic recombinant expression plasmid P-E2 was generated. The P-E2 was identified that the position of E2 gene of CSFV insert, the size and the reading frame were all right by restriction digestion and the sequence analysis. The P-E2 was used to transfect PK-15 cells in vitro by liposome infection protocol. The results of Western blot showed that the recombinant E2 protein was CSFV-specific. The fluorescence microscope detection indicated expressed E2 protein mainly located in plasma of PK-15 cells, which was the same as the results when PK-15 cells were infected by CSFV. These results above would provided basis for the pathogenesis of Classical swine fever virus.