Abstract:

The vascular-specific expression promoter BSP was cloned from the poplar genome in order to study the activity and tissue specificity, and the plant expression vector was constructed with the promoter and the green fluorescent protein (GFP) reporter gene, then transformed the tobacco leaves using the biolistic method. The trans-formed tobacco were cultured in hypertonic medium overnight, then transferred to the inducted media (MS+6-BA 0.5 mg/L+NAA 0.05 mg/L +Kan 100 mg/L) to take root and when they grown to a certain height, the section were prepared using the vein and root which had formed the vascular tissue. The green fluorescence of each tissue was observed by the fluorescence microscope of OLYPUS BX51/BX52 with the blue excitation, at the same time the untransformed tobacco was as control. Results indicated that the GFP gene driving by BSP promoter had been a high level of expression in vascular tissue cells in comparing with the other tissue and the control group. The transient expression of GFP in tobacco vascular tissues suggested that the promoter had the activity of vascular tissue-specific expression.