Abstract:

In this work, Lilium lancifolium bulbils with LSV viruses were used as experiment material. Stripping 0.2 mm size of the shoot apex after 36℃ pretreatment 10 d, then culturing them in MS+2.5 mg/L 6-BA+1.5 mg/L GA +0.5 mg/L NAA +0.1 g/L activated carbon +0.6% agar +3% sugar for bud induction, and detecting LSV virus using RT-PCR after a subculture, we found the virus-free rate to be 100%. The virus-free lily seedlings were put in MS +2.0 mg/L 6-BA +2.0 mg/L 2,4-D +0.1 mg/L NAA culture medium to induce callus and adventitious bud, induction rate 100%; in 1/2MS +2.5 mg/L 6-BA +0.2 mg/L NAA culture medium for differentiation and proliferation culture, proliferation multiples of Lilium lancifolium bulbils up to 4.31; in MS+1.2 mg/L NAA+0.5 g/L activated carbon culture medium for rooting, roots stout and branching vigorously, easy to survive after transplanting.