Abstract: SnRK2 (sucrose non-fermenting 1-related protein kinases 2) is a class of Ser/Thr protein kinase, which is unique to plants, involving in many signaling transductions and playing important roles in regulating environmental stress reponses. In this article, we employed the method of HMMER and phylogenetic tree, identified 16 SnRK2 members in apple genome, then we predicted and analyzed the evolutionary relationship, physical and chemical characteristics, secondary structures, motif, C-terminus sequences and EST, we also analyzed the relationship of apple SnRK2 gene family with those in Arabidopsis, rice, and maize. The result showed that the genes identified in apple, Arabidopsis, rice and maize are classified to three subfamilies, and analysis of phylogenetic tree, motif and C-terminus sequences showed that apple SnRK2 protein kinases are highly conserved comparing with those in other species. This research provides a bioinformatics foundation for the functions of the SnRK2 gene family in apple.