Abstract: In order to establish ‘Sanbai’ pomegranate in vitro regeneration system for genetic transformation and breeding, the tissue culture was carried out using stem segments with shoots as explants, which were cut from dormant woods. The results showed that the best primary culture medium was MS+ BA 2.0 mg/L+ IBA 0.2 mg/L+ PVP 2 g/L, of which the rate of budding reached up to 83.1% with the average number of shoots was 4.9, and the optimal proliferation medium was B5+ BA 0.8 mg/L+ IBA 0.5 mg/L+ AgNO3 0.2 mg/L+ GA3 0.2 mg/L+ CW (coconut water) 100 ml/L+ PVP 2 g/L，of which the proliferation index reached 5.6. The most suitable rooting media was B5+ IBA 1.0 mg/L+ CW 100 ml/L, of which the rooting rate was 95.6%, and the survival rate of transplantation could reach 87.3%. So the in vitro regeneration system of ‘Sanbai’ pomegranate was established..