Abstract: In order to fully utilize the fine traits of three pistils line and comprehensive analysis the genetic effects of pis1 gene on the agronomical characters, the main agronomic traits of wheat near isogentic lines, CSTP, CM28TP, MY29TP, NM9TP and their recurrent parents were investigated in this studies. The results showed that there were no obvious difference of plant height, stem diameter, flag leaf length, flag leaf width, effective panicle, spike length and spikelet number among near isogentic lines and their recurrent parents. The grain number per spike in near isogentic lines was superior to their recurrent parents except CSTP. But the 1000-kernel weight and plot yield lower than their recurrent parents. The positive correlation was found that plot yield was very significantly positive correlated with 1000-kernel weight. But the plot yield and 1000-kernel weight were no significant positive correlated with grain number per spike. In other words, it can not efficiently increase the yield only increase grain number per spike. So the priority target is to improve the 1000-kernel weight if use the three pistils line for breeding research. Cluster analysis indicated that the near isogentic lines and their corresponding parent can be classed into the same group. So the near isogentic lines were constructed successfully.