Abstract: To study the cultivate characteristics in different host, An IBDV strain was selected preliminary with Agar diffusion test, which collected from a chicken farm suspected infection with infectious bursal disease virus in Henan Province. The strain were infected susceptibility chicken, SPF chicken and DF1 cells. The results showed that, there were positive precipitation line in AGID; This strain could cause SPF chickens (morbidity with 100% and mortality with 60%) bursal swelling, bleeding, and even purple grape-like were showded; cause chick embryo CAM thickening, chicken embryos bleeding, cause the DF1 cells round off, the ELD50 and TCID50 was respectively 10-4.6/0.1 mL and 10-5.5/0.1 mL. The results confirmed that the isolated IBDV stain belongs to vvIBDV, it could replicate in chicken embryo and cell. It was named HN12.