Abstract: Field experiments were carried out to study effects of middle and low salt stresses on the biomass and physicological characteristics of different varieties of Helianthus tuberosus (‘Laiyu’, ‘Nanyu1’, ‘Anhui’) seedlings in Yellow River Delta. The results showed that, under middle salt stress, the height as well as biomass of the roots and aerial parts of all kinds of seedlings were lower than those under low salt stress, and Laiyu was higher than the others in biomass of the roots and aerial parts under both middle and low salt stress. Middle salt stress reduced the contents of leaf chlorophyll and protein to a certain extent. Laiyu was higher than others in the content of leaf chlorophyll under low salt stress, at the same time, Nanyu 1 was the highest under middle salt stress. Nanyu 1 was higher than others in the content of leaf protein under both middle and low salt stress. Middle salt stress increased the activities of SOD and POD, and increased the content of MDA and praline. Anhui was the highest in the content of MDA under both middle and low salt stress, Nanyu 1 was the highest in the content of praline under middle salt stress. In the climate conditions of Yellow River Delta, Laiyu seedling was most vigorous in mild saline-soil, meanwhile ‘Nanyu1 seedling was most vigorous in moderate saline-soil.