Differential Expression of PPARγ Gene in Different Tissues of Holstein Bulls

doi:10.11924/j.issn.1000-6850.2013-3236

Abstract

Abstract: The objective of this study was to compare the differential expression of the peroxisome proliferators-activated receptor-γ(PPARγ) gene in different tissues in two ages of Holstein bulls. Real-time fluorescent quantitative PCR was used to determine the relative expression of the PPARγ gene using RNA isolated from different adipose tissues (subcutaneous fat over the rib, subcutaneous fat over the sirloin, kidney fat, and mesenteric fat) and muscle tissues (longissimus dorsi and semitendinosus) in two ages (15 and 17 mo) of Holstein bulls. The results showed that, expression of PPARγ in mesenteric fat, kidney fat and subcutaneous sirloin fat was greater (P<0.05) than the longissimus dorsi and semitendinosus. And it was also greater (P<0.05) in mesenteric fat and kidney fat than the subcutaneous rib fat. But it had no difference between 15 and 17 mo in the same tissue. The expression of the PPARγ gene in tissues of Holstein bulls differed among sources of tissue. These results suggest that evaluation of gene expression of PPARγ gene should consider the sources of the tissue.

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