Abstract: The paper aims to study the variation of microclimate in the greenhouse where sweet cherry were planted, and provide agro- meteorology service for sweet cherry production. The observation trial of microclimate in fruit greenhouse was carried out in Linqu County of Shandong Province in 2012-2014. By contrast with the outside meteorological conditions, the characteristics of microclimate in fruit greenhouse were analyzed, and the prediction equations of microclimate in fruit greenhouse were established. The results showed that: during the observation period, daily average temperature, maximum temperature, minimum temperature and relative humidity in the fruit greenhouse were all higher than that of the outside. The highest value of air temperature and the minimum value of relative humidity in the greenhouse appeared in the afternoon, while the minimum air temperature and maximum relative humidity in the greenhouse generally appeared in the morning. The daily ranges of air temperature and relative humidity in the greenhouse were maxima on sunny days, and they were minima in overcast days. The microclimate in the greenhouse was significantly related to many meteorological factors of the outside on the day and the day before. The prediction equations of microclimate in fruit greenhouse were tested. The temperature prediction error was within 2℃, and the minimum relative humidity forecast error was less than 10%. The prediction effect was comparatively well.