Abstract: To explore the biomass and nutrient accumulation and accumulation law of fertilizer on banana, taking Brazil banana for example, get all body and divide them out, then get the biomass and nutrient of banana plant. The results showed that, the order of nutrient contents was K> Si> N> Ca> Mn> Mg> P> Fe> Zn> B> Cu. Compared with convention, fertilization could increase banana biomass, nearly 69.47%; could respectively increase accumulate nitrogen fertilizer 63.68%, phosphorus 64.12%g, potassium 5.07%, calcium 49.39%, magnesium 82.85%, manganese 26.60%, iron 76.24%, copper 44.49%, zinc 152.50%, boron 162.22%, silicon 81.18% per plant. The result showed fertilization can promote plant growth, increase nutrient accumulation of fruit and leaf, making suggestion for nutrient management scientifically of banana.