Abstract: In order to establish Dendrobium candidum micropropagation system, for the production of provide a reference of the Dendrobium candidum tissue culture in the process of sterilization methods, axillary bud induction, protocorm like body induction, proliferation and differentiation of the best culture medium formula. In Dendrobium mature stem segments as explants explore, 1 / 2MS medium or MS medium supplemented with different concentrations of hormone axillary bud induction of Dendrobium protocorm induce, proliferation, differentiation and rooting of seedlings, initially built Dendrobium tissue culture propagation technology system. Studies have shown that the most suitable medium axillary bud induction recipe is MS 6-BA 2.0 mg / L NAA 0.5 mg / L, The induction rate reached 91.28%, the average bud number was 2.34；the original bulb induce the most appropriate medium for 1 / 2MS 6-BA 0.5 mg / L NAA 0.5 mg / L 2,4-D 1.0 mg / L, Induction rate of 66.67%;PLB Multiplication most suitable medium for 1 / 2MS 6-BA 3.0mg / L NAA0.3mg / L, PLB optimum differentiation medium for 1 / 2MS NAA1 .0 mg / L 6-BA3.0 mg / L KT1.0mg / L, the most suitable rooting medium was 1 / 2MS 6-BA0mg / L NAA2.0mg / L AC0.5g / L .