Extraction Process of Polyphenols from Sargassum fusiforme and Its Antioxidant Activity in vitro

doi:10.11924/j.issn.1000-6850.casb15040163

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (32): 40-47.doi: 10.11924/j.issn.1000-6850.casb15040163

Special Issue: 园艺

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Extraction Process of Polyphenols from Sargassum fusiforme and Its Antioxidant Activity in vitro

Zhang Libin^1,2, Xiong Hejian³, Wu Jingna¹, Cai Shuilin¹, Liu Shuji¹, Qiao Kun¹, Liu Zhiyu¹

(¹Fisheries Reasearch Instiute of Fujian/Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province/Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen Fujian 361013; ²Dongshanxian Food and Drug Administration, Dongshan Fujian 363400;³School of Biotechnology, Jimei University, Xiamen Fujian 361021)

Received:2015-04-21 Revised:2015-10-28 Accepted:2015-08-25 Online:2015-11-16 Published:2015-11-16

Abstract

Abstract: To study extraction process of polyphenols from Sargassum fusiforme and its antioxidant activity in vitro, extraction conditions were optimized according to the polyphenols extraction rate by response surface methodology based on single factor experiment. Scavenging activity against DPPH, superoxide anion radicals, hydroxyl radicals and total antioxidant capacity of the extracts were evaluated. The results showed that extraction rate of polyphenols was 4.048% when the extracting time was 46 min, the temperature was 70℃,ethanol concentration was 40% and the ratio of liquid to material was 20:1. Crude extraction was purified by XDA-7 macroporous resin column and the content was 61.86%. The IC₅₀values of the obtained extracts against DPPH, superoxide anion radicals and hydroxyl radicals were 6.65, 6000 and 125.42 μg/mL. Total antioxidant capacity was 65.04 U/mg. These results suggested that polyphenols extracted from Sargassum fusiforme had great potential as a natural antioxidant.

CLC Number:

TS201.1

Zhang Libin,Xiong Hejian,Wu Jingna,Cai Shuilin,Liu Shuji,Qiao Kun and Liu Zhiyu. Extraction Process of Polyphenols from Sargassum fusiforme and Its Antioxidant Activity in vitro[J]. Chinese Agricultural Science Bulletin, 2015, 31(32): 40-47.

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Extraction Process of Polyphenols from Sargassum fusiforme and Its Antioxidant Activity in vitro

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