Abstract: In order to evaluate the suitable combination of sowing date and basic seedlings under the condition of rice and wheat annual rotation, this experiment was performed from 2012 to 2014 at Shuyang County, a northern area of Jiangsu Province. The growth period and yield formation of wheat were investigated under six different sowing dates (October 10th, 17th, 24th, 31st, and November 7th, 14th) and different basic seedlings (1.8,2.7, 3.6, 4.5 and 5.4 million /hm2). We found that the seedling emergence rates were affected by the sowing date, and it was low under a postponed sowing date. With the delay of the sowing date, the number of wheat leaves decreased and the total growth period of wheat was shortened, but the shortened days were less than the delayed days. Basic seedlings had little influence on the emergence rate and leaves age. At the same stage, the total tillers of wheat sown on the same sowing date increased with the basic seedlings increase, while those of wheat having the same basic seedlings decreased as the sowing date delayed. The plant height, length of internode below the ear and ear-length were significantly affected by the sowing date. With the sowing date being postponed, the yield increased firstly and decreased afterwards, and it was significantly affected by different sowing dates and basic seedlings. All in all, the suitable sowing dates of Huaibei wheat sown in the rice field were during October 17th-24th in cold winter while during 17th -31st in warm winter. The suitable basic seedlings of Huaibei wheat was 3.6 million per hectare.