Abstract: To explore the optimum application amount of different kinds of bio-organic fertilizers for high yield and quality of buckwheat varieties from Yunnan Province,‘Yunqiao-1’was used as the material to study the effects of three kinds of bio-organic fertilizers (F1, F2, F3) with three application rates (T1: 300 kg/hm2, T2: 600 kg/hm2, T3: 900 kg/hm2) on diseases, agronomic characters and yield of buckwheat. Results showed that all of the three kinds of fertilizers had positive effects on controlling buckwheat brown spot disease, among which, F3 had the best effect, but the application amounts had no significant difference on disease control; for agronomic characters and yield, the plant height and yield increased with the increase of application amounts, and the performance value increased along with the application amount increasing. The agronomic characters and yield all reached the maximum under T3; while there was no significant difference among the three kinds of fertilizers for all these characters. In summary, the incidence of buckwheat brown spot disease can be effectively reduced, the soil fertility can be improved, the plant growth and dry matter accumulation can be promoted and the yield can be significantly increased with the application of bio-organic fertilizer.