Structures and Biodiversity of Fungal Communities in Rhizosphere Soil of Root Rot Diseased Garlic

doi:10.11924/j.issn.1000-6850.casb19020043

Abstract

Abstract:

The aim is to investigate the difference in structure and biodiversity of rhizosphere fungi communities when root rot occurs, clarify the relationship between garlic root rot and the diversity change of rhizosphere soil fungus community, explore the microecological mechanism of root rot occurrence, and provide a theoretical basis for the prevention and control of the disease. High-throughput sequencing method was used to analyze the fungal ITS region sequences of the total DNA of garlic rhizosphere soil in the fields affected by garlic root rot for 3 consecutive years in Xinjiang. The results showed that Ascomycota and Zygomycota were the main phyla in rhizosphere soil of diseased garlic, and the high levels of Ascomycota were closely related to the occurrence of garlic root rot disease. Totally 137 genera were found by annotation, of which 19 genera had great correlation with garlic root rot. The abundance of Fusarium spp. in rhizosphere soil of diseased plants was significantly higher than that of healthy plants. With the increase of the onset time, the number and biodiversity of fungi decreased year by year. Garlic root rot disease is closely related to the structure and diversity of rhizosphere fungi community, and the change of the balance and diversity of soil fungi is a major cause of root rot.

Key words: garlic, root rot disease, high-throughput sequencing, rhizosphere soil, microbial diversity

CLC Number:

Q939.96

Xie Yuqing, Mao Jun, Wang Wei, Zhang Zhidong, Zhu Jing, Gu Meiying, Tang Qiyong, Song Suqin, Huang Wei, Wang Bo, Zhang Lijuan. Structures and Biodiversity of Fungal Communities in Rhizosphere Soil of Root Rot Diseased Garlic[J]. Chinese Agricultural Science Bulletin, 2020, 36(13): 145-153.

Figures/Tables 11

References 29

[1]	胡斌, 匡海学, 辛运杰 , 等. 大蒜降血脂作用及机制研究进展[J]. 中国实验方剂学杂志, 2019,25(8):181-186.
[2]	Beata Olas . Anti-Aggregatory Potential of Selected Vegetables-Promising Dietary Components for the Prevention and Treatment of Cardiovascular Disease[J]. Advances in Nutrition, 2019,10(2):280-290.
[3]	Ishani Bhaumik, Kunal Pal, Utsab Debnath , et al. Natural product inspired allicin analogs as novel anti-cancer agents[J]. Bioorganic Chemistry, 2019,86(5):259-272.
[4]	尚志梅, 张丰春, 赵斌 , 等. 大蒜提取物S-烯丙基-L-半胱氨酸对小鼠放疗损伤保护作用的研究[J]. 现代肿瘤医学, 2019(4):561-564.
[5]	Rodrigues C, Percival S S . Immunomodulatory Effects of Glutathione, Garlic Derivatives, and Hydrogen Sulfide[J]. Nutrients, 2019,11(2):295.
[6]	Mona K Galal, Ebtihal M M Elleithy, Mohamed I Abdrabou , et al. Modulation of caspase-3 gene expression and protective effects of garlic and spirulina against CNS neurotoxicity induced by lead exposure in male rats[J]. NeuroToxicology, 2019,72(5):15-28.
[7]	Li M, Wang S, Li X , et al. Diallyl sulfide treatment protects against acetaminophen-/carbon tetrachloride-induced acute liver injury by inhibiting oxidative stress, inflammation and apoptosis in mice[J]. Toxicol Res(Camb), 2018,8(1):67-76.
[8]	陈栋 . 北疆大蒜病毒病的调查和洋葱黄矮病毒的鉴定及序列分析[D]. 石河子:石河子大学, 2008.
[9]	谢玉清, 茆军, 朱静 , 等. 吉木萨尔县白皮大蒜病虫害调查研究[J]. 现代农业科技, 2013(1):115-117.
[10]	张丽娟, 王玮, 谢玉清 , 等. 新疆吉木萨尔地区大蒜根腐病病原菌的分离与鉴定[J]. 新疆农业科学, 2017,54(4):725-734.
[11]	马龙传, 王涛涛 . 金乡县大蒜根腐病发生及防治措施[J]. 农业科技通讯, 2018(6):339-340.
[12]	吕志军 . 大蒜根腐病发病原因及防治方法[N]. 河北科技报, 2018-04-26(005).
[13]	杨珍, 戴传超, 王兴祥 , 等. 作物土传真菌病害发生的根际微生物机制研究进展[J]. 土壤学报, 2018,11:1-12.
[14]	Andrew Lareen, Frances Burton, Patrick Schafer . Plant root-microbe communication in shaping root microbiomes[J]. Plant Mol Biol, 2016,90:575-587.
[15]	战斌慧, 周雪平 . 高通量测序技术在植物及昆虫病毒检测中的应用[J]. 植物保护, 2018,44(5):120-126,167.
[16]	Yu M, Jiang J L, Ren X M , et al. Research on relationship between occurrence of root rot and changes of fungal communities in rhizosphere of Panax quinquefolius[J]. China journal of Chinese materia medica, 2018,43(10):2038-2047.
[17]	王淑玉, 李小龙, 李红丽 , 等. 不同质地土壤烟株根际微生物菌群变化分析[J]. 湖南农业科学, 2015(3):5-9.
[18]	康捷, 章淑艳, 韩韬 , 等. 两种麻山药典型病害根际土壤微生物多样性研究[J]. 生物技术通报, 2017,33(7):107-113.
[19]	王殿东, 田雪亮, 潘丽梅 . 榨菜根肿病不同发病程度田根际土壤真菌群落多样性研究[J]. 北方园艺, 2016(13):115-118.
[20]	涂祖新, 张莉莉, 贺伟华 , 等. 赣南脐橙黄龙病株与健康株的根际土壤真菌群落多样性比较[J]. 安徽农业大学学报, 2017,44(2):333-341.
[21]	肖蓉, 曹秋芬, 聂园军 , 等. 基于高通量测序患炭疽病草莓根际与健康草莓根际细菌群落的比较研究[J]. 中国农学通报, 2017,33(11):14-20.
[22]	Tedersoo, Bahram M, Põlme S , et al. Global diversity and geography of soil fungi[J]. Science, 2014,346:1-11.
[23]	李小龙, 李红丽, 曾强 , 等. 钙镁磷肥对青枯病发病烟株根际土壤微生物区系的影响[J]. 中国烟草学报, 2016,22(1):71-75.
[24]	Li X G, Ding C F, Zhang T L , et al. Fungal pathogen accumulation at the expense of plant-beneficial fungi as a consequence of consecutive peanut monoculturing[J]. Soil Biology & Biochemistry, 2014,72:11-18.
[25]	谭雪莲, 郭天文, 刘高远 . 马铃薯连作土壤微生物特性与土传病原菌的相互关系[J]. 灌溉排水学报, 2016,35(8):30-35.
[26]	蔡柏岩, 王丽阳, 胡维 , 等. 连作大豆苗期根际土壤根腐病病原真菌菌群结构分析[J]. 中国农学通报, 2015,31(32):92-98.
[27]	Papanikolaou S, Aggelis G . Sources of microbial oils with emphasis to Mortierella (Umbelopsis) isabellina fungus[J]. World J Microbiol Biotechnol, 2019,35:63.
[28]	李芳 . 长期不同是非条件下黄淮海平原干旱土壤微生物群落结构特征的演变[D]. 郑州:河南农业大学, 2018.
[29]	Ellegaard-Jensen L, Aamand J, Kragelund B B , et al. Strains of the soil fungi Mortierella show different degradation potentials for the phenylurea herbicide diuron[J]. Biodegradation, 2013,24:765.

样品编号	物种数	物种多样性	物种均匀度	物种丰度	测序深度	系统发育多样性
J1	559	5.968	0.965	593.628	0.999	205.432
J2	463	6.510	0.977	489.562	0.999	191.735
J3	525	4.976	0.928	571.500	0.998	199.376
J4	361	5.750	0.957	396.467	0.999	172.652
J5	418	3.788	0.81	452.628	0.999	196.448
J6	245	1.456	0.284	265.523	0.999	120.483
B2	422	6.165	0.972	452.611	0.999	169.594
B3	419	4.012	0.823	474.338	0.998	163.221
B4	349	5.639	0.956	359.014	0.999	154.986
B5	286	3.042	0.65	324.936	0.999	137.946
B6	248	3.051	0.623	274.000	0.999	132.064
J（健康组）	429	4.741	0.820	461.551	0.999	181.021
B（病害组）	345	4.382	0.805	376.980	0.999	151.562

样品编号	物种数	物种多样性	物种均匀度	物种丰度	测序深度	系统发育多样性
J1	559	5.968	0.965	593.628	0.999	205.432
J2	463	6.510	0.977	489.562	0.999	191.735
J3	525	4.976	0.928	571.500	0.998	199.376
J4	361	5.750	0.957	396.467	0.999	172.652
J5	418	3.788	0.81	452.628	0.999	196.448
J6	245	1.456	0.284	265.523	0.999	120.483
B2	422	6.165	0.972	452.611	0.999	169.594
B3	419	4.012	0.823	474.338	0.998	163.221
B4	349	5.639	0.956	359.014	0.999	154.986
B5	286	3.042	0.65	324.936	0.999	137.946
B6	248	3.051	0.623	274.000	0.999	132.064
J（健康组）	429	4.741	0.820	461.551	0.999	181.021
B（病害组）	345	4.382	0.805	376.980	0.999	151.562

Taxonomy	分组			样品
Taxonomy	J	B	B/J	J1	J2	J3	J4	J5	J6	B2	B3	B4	B5	B6
Inocybe	15	0	0.00	0	0	0	89	0	0	0	0	0	0	0
Udeniomyces	11	0	0.00	64	0	0	0	0	0	0	0	0	0	0
Pholiotina	10	0	0.00	9	0	0	0	50	0	0	0	0	0	0
Basidiobolus	105	0	0.00	0	0	628	0	0	0	1	0	0	0	0
Lachnella	20	0	0.01	8	0	96	0	14	0	0	1	0	0	0
Phaeosphaeria	43	1	0.01	258	0	0	0	0	0	0	2	1	0	0
Coprinopsis	27	0	0.01	0	163	0	0	1	0	0	1	0	1	0
Botrytis	5181	190	0.04	295	134	497	130	29791	239	185	545	114	68	39
Kernia	42	2	0.04	17	206	3	4	20	2	2	4	2	1	0
Nematoctonus	79	4	0.06	40	431	3	0	1	0	0	21	0	1	0
Preussia	620	47	0.08	67	3107	79	424	18	23	92	63	11	23	46
Peziza	166	16	0.10	82	0	765	0	148	0	1	80	1	0	0
Tricladium	1	23	34.50	1	1	1	0	1	0	115	0	0	0	0
Wickerhamomyces	508	24680	48.61	586	578	1385	244	252	1	591	30778	2566	43009	46454
Leucosporidium	0	11	63.60	0	1	0	0	0	0	0	0	0	0	53
Scutellinia	14	928	64.74	9	8	60	3	6	0	6	1217	829	2	2586
Actinomucor	6	609	96.09	8	12	8	6	4	0	10	64	2738	226	5
Talaromyces	0	34	202.80	0	0	1	0	0	0	0	0	169	0	0
Torula	0	20	-	0	0	0	0	0	0	0	0	100	0	0

Taxonomy	分组			样品
Taxonomy	J	B	B/J	J1	J2	J3	J4	J5	J6	B2	B3	B4	B5	B6
Inocybe	15	0	0.00	0	0	0	89	0	0	0	0	0	0	0
Udeniomyces	11	0	0.00	64	0	0	0	0	0	0	0	0	0	0
Pholiotina	10	0	0.00	9	0	0	0	50	0	0	0	0	0	0
Basidiobolus	105	0	0.00	0	0	628	0	0	0	1	0	0	0	0
Lachnella	20	0	0.01	8	0	96	0	14	0	0	1	0	0	0
Phaeosphaeria	43	1	0.01	258	0	0	0	0	0	0	2	1	0	0
Coprinopsis	27	0	0.01	0	163	0	0	1	0	0	1	0	1	0
Botrytis	5181	190	0.04	295	134	497	130	29791	239	185	545	114	68	39
Kernia	42	2	0.04	17	206	3	4	20	2	2	4	2	1	0
Nematoctonus	79	4	0.06	40	431	3	0	1	0	0	21	0	1	0
Preussia	620	47	0.08	67	3107	79	424	18	23	92	63	11	23	46
Peziza	166	16	0.10	82	0	765	0	148	0	1	80	1	0	0
Tricladium	1	23	34.50	1	1	1	0	1	0	115	0	0	0	0
Wickerhamomyces	508	24680	48.61	586	578	1385	244	252	1	591	30778	2566	43009	46454
Leucosporidium	0	11	63.60	0	1	0	0	0	0	0	0	0	0	53
Scutellinia	14	928	64.74	9	8	60	3	6	0	6	1217	829	2	2586
Actinomucor	6	609	96.09	8	12	8	6	4	0	10	64	2738	226	5
Talaromyces	0	34	202.80	0	0	1	0	0	0	0	0	169	0	0
Torula	0	20	-	0	0	0	0	0	0	0	0	100	0	0