Ubiquitin Modification and Its Research Progress in Plant Proteomics

doi:10.11924/j.issn.1000-6850.casb2020-002

Abstract

Abstract:

Ubiquitination modification is an important post-translational modification of proteins, which participates in the regulation of many important life activities in cells. In recent years, with the development of ubiquitinated proteins enrichment technology and mass spectrometry technology, great progress has been made in ubiquitination proteomics. At present, there are many ubiquitination proteomics studies in animals and yeasts, but few in plants. In order to summarize the latest research results of ubiquitination proteomics in plants, the ubiquitination modifications, the identification of ubiquitination sites by mass spectrometry, the enrichment methods of ubiquitination proteins and the application of ubiquitination proteomics in plant light regulation, hormone treatment and stress conditions were reviewed in this paper, which could provide the rationale for the latest research of ubiquitination modifications in plants.

Key words: ubiquitination modifications, ubiquitinated proteins enrichment, mass spectrometry, plant proteomics

CLC Number:

S184

Zhang Jialin, Li Haiying. Ubiquitin Modification and Its Research Progress in Plant Proteomics[J]. Chinese Agricultural Science Bulletin, 2020, 36(30): 75-81.

Figures/Tables 3

References 48

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蛋白质/ 肽段	UBD	特异性
NEMO	UBA	M1>K63 泛素链
TAB2	NZF	K63泛素链
Ubiquilin-1	UBA	所有泛素链
MultiDsk	UBA	所有泛素链
RAP80	双重UIM	K63泛素链
TRABID	NZF	K29和K33泛素链

蛋白质/ 肽段	UBD	特异性
NEMO	UBA	M1>K63 泛素链
TAB2	NZF	K63泛素链
Ubiquilin-1	UBA	所有泛素链
MultiDsk	UBA	所有泛素链
RAP80	双重UIM	K63泛素链
TRABID	NZF	K29和K33泛素链

植物材料		组织	处理条件	富集方法		结果		参考文献
拟南芥 (Arabidopsis thaliana L.)		7日龄的黄化拟南芥幼苗	红光照射	GST-UBA亲和介质法泛素偶联His标签法		照射前130种泛素化蛋白照射后302种泛素化蛋白		[42]
玉米(Zea mays L.)		7日龄玉米黄化幼苗	光照射	K-ε-GG抗体法		1053种泛素化蛋白 1926个泛素化位点 78个差异表达泛素化位点		[43]
矮牵牛 (Petunia hybrida Mitchell)	花冠		乙烯处理		K-ε-GG抗体法		284个下调蛋白 233个上调蛋白	[44]
非洲水稻 (Oryza glaberrima)	12日龄的非洲水稻幼苗		45℃ 高温处理		K-ε-GG抗体法		264个上调肽段 147个下调肽段	[45]
马铃薯四倍体栽培品种 (Solanum tuberosum L.)	块茎培育的组培苗		聚乙二醇处理模拟干旱胁迫		K-ε-GG抗体法		3个下调位点 22个上调位点	[46]
茶 (Camellia sinensis L.)	两年龄的茶		干旱处理		K-ε-GG抗体法		14个上调位点 123个下调位点	[47]
水稻(Oryza sativa L.)	7日龄水稻幼苗		几丁质处理		K-ε-GG抗体法		144个位点上调 167个位点下调	[48]
水稻(Oryza sativa L.)	7日龄水稻幼苗		flg22处理		K-ε-GG抗体法		151个为位点上调 179个位点下调	[48]

植物材料		组织	处理条件	富集方法		结果		参考文献
拟南芥 (Arabidopsis thaliana L.)		7日龄的黄化拟南芥幼苗	红光照射	GST-UBA亲和介质法泛素偶联His标签法		照射前130种泛素化蛋白照射后302种泛素化蛋白		[42]
玉米(Zea mays L.)		7日龄玉米黄化幼苗	光照射	K-ε-GG抗体法		1053种泛素化蛋白 1926个泛素化位点 78个差异表达泛素化位点		[43]
矮牵牛 (Petunia hybrida Mitchell)	花冠		乙烯处理		K-ε-GG抗体法		284个下调蛋白 233个上调蛋白	[44]
非洲水稻 (Oryza glaberrima)	12日龄的非洲水稻幼苗		45℃ 高温处理		K-ε-GG抗体法		264个上调肽段 147个下调肽段	[45]
马铃薯四倍体栽培品种 (Solanum tuberosum L.)	块茎培育的组培苗		聚乙二醇处理模拟干旱胁迫		K-ε-GG抗体法		3个下调位点 22个上调位点	[46]
茶 (Camellia sinensis L.)	两年龄的茶		干旱处理		K-ε-GG抗体法		14个上调位点 123个下调位点	[47]
水稻(Oryza sativa L.)	7日龄水稻幼苗		几丁质处理		K-ε-GG抗体法		144个位点上调 167个位点下调	[48]
水稻(Oryza sativa L.)	7日龄水稻幼苗		flg22处理		K-ε-GG抗体法		151个为位点上调 179个位点下调	[48]