Effects of Different Long-term Fertilization Modes on Photosynthetic Carbon Distribution in Sweetpotato Cropping System

doi:10.11924/j.issn.1000-6850.casb2022-0452

Abstract

Abstract:

The ¹³CO₂ pulse labeling technique was used to trace ¹³C in sweetpotato cropping system and evaluate the effects of 4 fertilization modes (CK: no fertilization, NPK: combined application of N, P and K fertilizer, M: organic fertilizer, MNPK: combination of N, P, K and organic fertilizer) on the distribution of photosynthetic carbon based on 40-year long-term fertilization experiment in Xuzhou of Jiangsu Province. The results showed that fertilizer application significantly increased the biomass and dry matter weight of sweetpotato, and the increasing range of MNPK treatment was higher than that of other fertilization treatments. The carbon was quickly translocated and unevenly distributed in the sweetpotato cropping system on D1 (the first day after labeling), following the order of leaf, petiole>stem, root>bulk soil, and the δ¹³C value in the aboveground parts under no fertilization was significantly higher than that under fertilization. The δ¹³C value in various sweetpotato plant organs decreased from D1 to D30 (the 30th day after labeling), with the distribution ratio ranged from 19.38% to 31.44% in the aboveground parts, and from 60.19% to 71.86% in roots; while the δ¹³C value in bulk soil increased slightly after labeling, with the distribution ratio ranged from 8.05% to 11.11%. Fertilizer application significantly increased the content of ¹³C in the roots compared with no fertilization. And the content of ¹³C in roots under MNPK treatment was significantly higher than that under NPK and M treatments, indicating that fertilizer application during the expansion stage of sweetpotato was beneficial to the accumulation of photosynthetic carbon in roots, and the combined application of organic and inorganic fertilizers had significant cumulative effect.

Key words: long-term fertilization, sweetpotato, ¹³C labeling, photosynthetic carbon, distribution

WEI Meng, ZHAO Peng, JIA Zhihang, JIANG Wei, ZHANG Jia, TANG Zhonghou, ZHANG Aijun. Effects of Different Long-term Fertilization Modes on Photosynthetic Carbon Distribution in Sweetpotato Cropping System[J]. Chinese Agricultural Science Bulletin, 2023, 39(15): 85-91.

Figures/Tables 6

References 25

[1]	TONG X G, XU M G, WANG X J, et al. Long-term fertilization effects on organic carbon fractions in a red soil of China[J]. Catena, 2014, 113:251-259. doi: 10.1016/j.catena.2013.08.005 URL
[2]	GUILLAUME T, DAMRIS M, KUZYAKOV Y. Losses of soil carbon by converting tropical forest to plantations: erosion and decomposition estimated by δ¹³C[J]. Global change biology, 2015, 21(9):3548-3560. doi: 10.1111/gcb.2015.21.issue-9 URL
[3]	谭立敏, 彭佩钦, 李科林, 等. 水稻光合同化碳在土壤中的矿化和转化动态[J]. 环境科学, 2014, 35(1):233-239.
[4]	吴小红, 简燕, 陈晓娟, 等. 农田土壤自养微生物的CO₂同化功能及同化碳转化的分子生态学机制[J]. 生态学报, 2014, 34(3):1-8.
[5]	AN T T, SCHAEFFER S, LI S Y, et al. Carbon fluxes from plants to soil and dynamics of microbial immobilization under plastic film mulching and fertilizer application using ¹³C pulse-labeling[J]. Soil biology and biochemistry, 2015, 80:53-61. doi: 10.1016/j.soilbio.2014.09.024 URL
[6]	田慎重, 王瑜, 宁堂原, 等. 转变耕作方式对长期旋免耕农田土壤有机碳库的影响[J]. 农业工程学报, 2016, 32(17):98-105.
[7]	TANG Z H, ZHANG A J, WEI M, et al. Physiological response to potassium deficiency in three sweet potato (Ipomoea batatas [L.] Lam.) genotypes differing in potassium utilization efficiency[J]. Acta physiologiae plantarum, 2015, 37:1-10. doi: 10.1007/s11738-014-1746-y URL
[8]	安婷婷, 汪景宽, 李双异, 等. 用¹³C脉冲标记方法研究施肥与地膜覆盖对玉米光合碳分配的影响[J]. 土壤学报, 2013, 50(5):948-955.
[9]	JIN J, WANG G H, LIU J D, et al. Seasonal allocation of photosynthetically fixed carbon to the soybean-grown mollisols in Northeast China[J]. Crop and pasture science, 2011, 62(7):563-570. doi: 10.1071/CP11006 URL
[10]	GE T D, LIU C, YUAN H Z, et al. Tracking the photosynthesized carbon input into soil organic carbon pools in a rice soil fertilized with nitrogen[J]. Plant and soil, 2015, 392(1/2):17-25. doi: 10.1007/s11104-014-2265-8 URL
[11]	邓扬悟, 唐纯, 袁红朝, 等. ¹³C脉冲标记法:不同生育期水稻光合碳在植物-土壤系统中的分配[J]. 生态学报, 2017, 37(19):6466-6471.
[12]	聂三安, 周萍, 葛体达, 等. 水稻光合同化碳向土壤有机碳库输入的定量研究:¹⁴C连续标记法[J]. 环境科学, 2012, 33(4):1346-1351.
[13]	祝贞科, 沈冰洁, 葛体达, 等. 农田作物同化碳输入与周转的生物地球化学过程[J]. 生态学报, 2016, 36(19):5987-5997.
[14]	MOORE-KUCERA J, DICK R P. Application of ¹³C-labeled litter and root materials for in situ decomposition studies using phospholipid fatty acids[J]. Soil biology and biochemistry, 2008,40,2485-2493.
[15]	BUTLER J L, BOTTOMLEY P J, GRIFFITH S M, et al. Distribution and turnover of recently fixed photosynthate in ryegrass rhizospheres[J]. Soil biology and biochemistry, 2004, 36(2):371-382. doi: 10.1016/j.soilbio.2003.10.011 URL
[16]	乔云发, 韩晓增, 赵兰坡. 长期定量施肥对玉米光合碳分配的影响[J]. 水土保持学报, 2010, 24(4):208-212.
[17]	尹云峰, 杨玉盛, 高人, 等. 植物富集¹³C标记技术的初步研究[J]. 土壤学报, 2010, 47(4):790-793.
[18]	LU Y, WATANABE A, KIMURA M. Input and distribution of photosynthesized carbon in a flooded rice soil[J]. Global biogeochemical cycles, 2002, 16(4):32-1-32-8.
[19]	LEAKE J R, OSTLE N J, RANGEL-CASTRO J I, et al. Carbon fluxes from plants through soil organisms determined by field ¹³CO₂ pulse-labelling in an upland grassland[J]. Applied soil ecology, 2006, 33(2):152-175. doi: 10.1016/j.apsoil.2006.03.001 URL
[20]	刘萍, 汪春玉, 李忠佩. ¹³C脉冲标记定量研究施氮量对光合碳在水稻-土壤系统中分布的影响[J]. 土壤学报, 2015, 52(3):567-575.
[21]	KUZYAKOV Y, DOMANSKI G. Carbon input by plants into the soil[J]. Journal of plant nutrition and soil science, 2000, 163:421-431. doi: 10.1002/(ISSN)1522-2624 URL
[22]	于鹏, 张玉玲, 王春新, 等. 不同生育期光合碳在水稻-土壤系统中的分配[J]. 土壤学报, 2017, 54(5):1218-1229.
[23]	YUAN H Z, ZHU Z K, GE T D, et al. Microbial utilization of rice root exudates: ¹³C labeling and PLFA composition[J]. Biology and fertility of soils, 2016, 52:615-627. doi: 10.1007/s00374-016-1101-0 URL
[24]	GE T D, LI B Z, ZHU Z K, et al. Rice rhizodeposition and its utilization by microbial groups depends on N fertilization[J]. Biology and fertility of soils, 2016, 53(1):37-48. doi: 10.1007/s00374-016-1155-z URL
[25]	CANARINI A, DIJKSTRA F A. Dry-rewetting cycles regulate wheat carbon rhizodeposition, stabilization and nitrogen cycling[J]. Soil biology and biochemistry, 2015, 81:195-203. doi: 10.1016/j.soilbio.2014.11.014 URL

处理	N	P₂O₅	K₂O	有机肥
CK	0	0	0	0
NPK	300	150	225	0
M	0	0	0	37500
MNPK	300	150	225	37500

处理	N	P₂O₅	K₂O	有机肥
CK	0	0	0	0
NPK	300	150	225	0
M	0	0	0	37500
MNPK	300	150	225	37500

处理	pH (2.5:1)	电导率EC/(μS/cm)	有机碳SOC/(g/kg)	全氮TN/(g/kg)	有效磷AP/(mg/kg)	速效钾AK/(mg/kg)
CK	7.95	356.73	8.42	0.73	5.84	38.08
NPK	7.91	130.78	13.06	1.22	12.87	92.11
M	7.94	77.32	17.63	1.48	173.84	65.08
MNPK	7.82	133.95	18.18	1.67	131.45	124.56

处理	pH (2.5:1)	电导率EC/(μS/cm)	有机碳SOC/(g/kg)	全氮TN/(g/kg)	有效磷AP/(mg/kg)	速效钾AK/(mg/kg)
CK	7.95	356.73	8.42	0.73	5.84	38.08
NPK	7.91	130.78	13.06	1.22	12.87	92.11
M	7.94	77.32	17.63	1.48	173.84	65.08
MNPK	7.82	133.95	18.18	1.67	131.45	124.56

处理		生物量					干物质量
		地上部			块根		地上部			块根
				叶片	块根	叶柄	藤蔓			块根	叶片	叶柄	藤蔓
1 d	CK	25.23±5.67c	22.48±4.00c	19.31±1.85b	132.89±14.81c		4.51±1.20c	2.03±0.37c	2.99±0.18b	34.79±4.33c
	NPK	44.24±1.83b	61.75±10.26b	85.42±12.71a	268.36±20.38b		7.59±0.32b	4.95±0.83b	11.99±1.72a	66.42±7.92b
	M	47.41±6.65b	70.51±4.54ab	78.48±20.47a	332.71±75.36ab		8.21±1.28b	5.47±0.27b	11.13±3.24a	83.89±10.44b
	MNPK	66.86±8.69a	79.77±5.27a	95.06±32.92a	393.62±42.75a		10.97±1.45a	6.72±0.89a	13.31± 3.44a	103.17±11.98a
30 d	CK	57.84±13.94b	30.05±7.07c	27.82±6.67c	231.09±96.36c		12.37±1.22b	3.09±0.49c	5.50±0.65b	81.32±23.55c
	NPK	148.94±88.80a	106.72±66.33b	105.60±47.46b	622.25±243.66b		32.72±8.96a	8.68±2.88b	21.28±3.67a	201.24±32.72b
	M	121.15±40.14a	121.11±41.97ab	121.18±13.64ab	774.39±201.87b		22.80±1.93a	10.66±2.00ab	18.92±0.49a	232.10±23.36b
	MNPK	157.80±68.15a	168.89±70.79a	146.68±51.96a	994.47±309.72a		28.49±8.26a	13.13±2.84a	21.09±5.14a	313.33±39.25a