Chinese Agricultural Science Bulletin ›› 2023, Vol. 39 ›› Issue (27): 103-109.doi: 10.11924/j.issn.1000-6850.casb2022-0814
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LUO Qinchuan(), SUN Houjun, TANG Wei, XIE Yiping, YANG Dongjing, MA Jukui, CHEN Jingwei, GAO Fangyuan, ZHANG Chengling()
Received:
2022-09-26
Revised:
2022-12-15
Online:
2023-09-25
Published:
2023-09-22
LUO Qinchuan, SUN Houjun, TANG Wei, XIE Yiping, YANG Dongjing, MA Jukui, CHEN Jingwei, GAO Fangyuan, ZHANG Chengling. Cel61A Gene in Ceratocystis fimbriata: Cloning and Expression Analysis[J]. Chinese Agricultural Science Bulletin, 2023, 39(27): 103-109.
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.casb2022-0814
引物名称 | 引物序列(5’→3’) | 退火温度/℃ | 片段长/备注 |
---|---|---|---|
Cfcel61A-F | GCCAAGGCACGCATGAGAAGAG | 58 | 1155 bp/基因全长 |
Cfcel61A-R | CACCTCCTCACAATCTTCTC | ||
CfCe61q-F | ACTGGAGTGACTGGCCCGATTC | 60 | 219 bp/荧光定量 |
CfCe61q-R | GCCAGCGAGGTAGGGATCTGT | ||
CfActin-F | AGAAGGACTCGTACGTTGGTG | 60 | 209 bp/内参基因荧光定量 |
CfActin-R | TCTTCTCACGGTTGGACTTG |
引物名称 | 引物序列(5’→3’) | 退火温度/℃ | 片段长/备注 |
---|---|---|---|
Cfcel61A-F | GCCAAGGCACGCATGAGAAGAG | 58 | 1155 bp/基因全长 |
Cfcel61A-R | CACCTCCTCACAATCTTCTC | ||
CfCe61q-F | ACTGGAGTGACTGGCCCGATTC | 60 | 219 bp/荧光定量 |
CfCe61q-R | GCCAGCGAGGTAGGGATCTGT | ||
CfActin-F | AGAAGGACTCGTACGTTGGTG | 60 | 209 bp/内参基因荧光定量 |
CfActin-R | TCTTCTCACGGTTGGACTTG |
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