Study on Pollen Viability and Stigma Acceptability of Guava

doi:10.11924/j.issn.1000-6850.casb2023-0415

Abstract

Abstract:

In order to explore the optimal period for pollen collection, pollination and emasculation in guava, fresh guava pollen was used as the experimental material and an orthogonal design experiment was conducted to screen the optimal formula of the culture medium. Subsequently, the pollen viability and stigma receptivity were determined at different preservation time and states of openness. The results showed that the influence of culture medium formulas on the pollen viability of guava was in the order of sugar> boric acid> calcium chloride. The optimal treatment combination was 150 g/L sucrose+ 0 g/L boric acid+ 0.3 g/L calcium chloride with a pollen viability of 91.9%. The pollen viability decreased with prolonged storage time and the pollen viability of freshly harvested guava pollen was 89.69%, which decreased to 0% after 96 hours of storage. Guava flowers had the highest pollen viability in stage Ⅲ (complete bud split and sepal shedding), and the stigma receptivity was the strongest in stage Ⅳ (petal spreading). Moreover, guava flowers in stage Ⅰ and Ⅱ were in the states of unopened anthers, no pollen adhesion on the stigma and a low rate of pollen viability. Therefore, the optimal emasculation period is stages Ⅰ and Ⅱ, while the optimal periods of pollination and pollen collection are stage Ⅳ and stage Ⅲ, respectively.

Key words: guava, culture in vitro, pollen viability, stigma receptivity, optimal pollination period, optimal pollen collection period, optimal emasculation period

QIN Jian, JIANG Haoran, ZHANG Shoumei, LAI Duo, XIAO Weiqiang, ZHUANG Qingli, SHAO Xuehua. Study on Pollen Viability and Stigma Acceptability of Guava[J]. Chinese Agricultural Science Bulletin, 2024, 40(13): 70-75.

Figures/Tables 10

References 33

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时期	外观特征
时期Ⅰ	花苞膨大、顶端较尖
时期Ⅱ	花苞裂开、微露白色花瓣
时期Ⅲ	花苞完全裂开、萼片脱落，花瓣未展开
时期Ⅳ	花瓣展开、露出雄蕊和雌蕊

时期	外观特征
时期Ⅰ	花苞膨大、顶端较尖
时期Ⅱ	花苞裂开、微露白色花瓣
时期Ⅲ	花苞完全裂开、萼片脱落，花瓣未展开
时期Ⅳ	花瓣展开、露出雄蕊和雌蕊

水平	因素
水平	A.蔗糖浓度/(g/L)	B.硼酸浓度/(g/L)	C.氯化钙浓度/(g/L)
1	0	0	0
2	50	0.2	0.1
3	100	0.4	0.2
4	150	0.6	0.3

水平	因素
水平	A.蔗糖浓度/(g/L)	B.硼酸浓度/(g/L)	C.氯化钙浓度/(g/L)
1	0	0	0
2	50	0.2	0.1
3	100	0.4	0.2
4	150	0.6	0.3

处理	因素			花粉生活力/%
处理	A	B	C	重复1	重复2	重复3	均值
1	0	0	0	29.8	26.5	30.4	28.9
2	0	0.2	0.1	19.2	20.0	20.0	19.7
3	0	0.4	0.2	10.0	11.3	10.3	10.5
4	0	0.6	0.3	5.3	6.7	7.5	6.5
5	50	0	0.1	48.0	48.2	50.0	48.7
6	50	0.2	0	25.0	18.0	18.2	20.4
7	50	0.4	0.3	33.3	38.1	36.8	36.1
8	50	0.6	0.2	26.1	28.6	26.7	27.1
9	100	0	0.2	60.0	59.4	60.7	60.0
10	100	0.2	0.3	65.0	60.0	60.7	61.9
11	100	0.4	0	50.0	47.6	48.0	48.5
12	100	0.6	0.1	42.1	42.1	45.0	43.1
13	150	0	0.3	91.4	93.3	90.9	91.9
14	150	0.2	0.2	55.6	62.5	60.0	59.4
15	150	0.4	0.1	30.8	30.0	30.0	30.3
16	150	0.6	0	27.5	23.5	26.3	25.8
均值K₁	16.400	57.375	30.900
均值K₂	33.075	44.275	35.450
均值K₃	53.375	31.325	39.250
均值K₄	51.850	25.625	49.100
极值R	36.975	31.750	18.200