Effect of Ultra-dry Treatment on Seed Viability and Physiological and Biochemical Activities of Common Beans

doi:10.11924/j.issn.1000-6850.casb2023-0316

Abstract

Abstract:

The aims of this study was to apply the method of silica gel drying to the ultra-dry treatment of common beans, and analyze the main physiological activity related indexes, in order to provide scientific basis and practical reference for the long-term storage of beans seeds. After ultra-dry treatment, indicators such as protein content and dehydrogenase (ADH) content were measured using Coomassie Brilliant Blue G-250 staining, TTC method, thiobarbituric acid (TBA) method, etc. The results showed that when the water content of ‘Jiangjun’ was 6.95%, the seed vitality and physiological indicators were higher than that of the control, while the activity of antioxidant enzymes was significantly lower than that of the control. When the water content of ‘Ziguan’ was 6.89%, the physiological indexes were higher than that of the control, and the content of O₂^- decreased. When the water content of ‘Renaya’ was 6.15%, the germination rate was the highest, the protein content and ADH activity increased significantly, and the content of O₂^- and the MDA were not significant. These results indicated that moderate ultra-dry treatment had a greater effect on ‘Jiangjun’ seeds, and little or no effect on ‘Ziguan’ and ‘Renaya’ seeds. The results showed that the ultra-dry treatment was feasible for the preservation of common bean seeds, which laid the foundation for the long-term storage of common bean seeds.

Key words: ultra-drying treatment, common bean, seed viability, physiological activity, storage period

HAN Jimin, SUN Lu, FENG Guojun, LIU Dajun, LIU Chang, YANG Xiaoxu. Effect of Ultra-dry Treatment on Seed Viability and Physiological and Biochemical Activities of Common Beans[J]. Chinese Agricultural Science Bulletin, 2024, 40(15): 66-72.

Figures/Tables 7

References 28

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品种	含水量/%	发芽率/%	硬实率/%	发芽指数	活力指数
将军	12.44(ck)	98.67±1.89a	1.33±0.94	59.08±4.78ab	3.45±0.14b
	8.52	83.33±6.59b	3.33±0.94	48.41±5.59b	3.04±0.44b
	6.95	92.00±1.63a	6.00±1.63	64.60±7.28a	4.59±0.69a
	5.78	50.66±0.94c	34±3.26	28.63±0.60c	1.28±0.13b
紫冠	12.77(ck)	87.33±4.11a	0.67±0.01	44.83±3.68a	1.75±0.26b
	8.38	63.33±4.98c	5.33±0.94	34.66±2.17b	1.35±0.00b
	6.89	78.00±2.82b	7.33±0.94	38.54±2.06b	1.37±0.05b
	5.35	79.33±2.49ab	8.00±1.63	47.40±1.00a	2.69±0.27a
热那亚	11.87(ck)	89.33±3.39ab	0	52.43±0.42c	2.27±0.21b
	8.11	97.33±0.94a	0	64.60±0.21b	2.54±0.14b
	6.15	96.00±0.00a	0	78.12±3.56a	3.24±0.04a
	5.01	80.67±6.80b	0	52.24±1.21c	2.36±0.08b

品种	含水量/%	发芽率/%	硬实率/%	发芽指数	活力指数
将军	12.44(ck)	98.67±1.89a	1.33±0.94	59.08±4.78ab	3.45±0.14b
	8.52	83.33±6.59b	3.33±0.94	48.41±5.59b	3.04±0.44b
	6.95	92.00±1.63a	6.00±1.63	64.60±7.28a	4.59±0.69a
	5.78	50.66±0.94c	34±3.26	28.63±0.60c	1.28±0.13b
紫冠	12.77(ck)	87.33±4.11a	0.67±0.01	44.83±3.68a	1.75±0.26b
	8.38	63.33±4.98c	5.33±0.94	34.66±2.17b	1.35±0.00b
	6.89	78.00±2.82b	7.33±0.94	38.54±2.06b	1.37±0.05b
	5.35	79.33±2.49ab	8.00±1.63	47.40±1.00a	2.69±0.27a
热那亚	11.87(ck)	89.33±3.39ab	0	52.43±0.42c	2.27±0.21b
	8.11	97.33±0.94a	0	64.60±0.21b	2.54±0.14b
	6.15	96.00±0.00a	0	78.12±3.56a	3.24±0.04a
	5.01	80.67±6.80b	0	52.24±1.21c	2.36±0.08b