Distribution of Six Rice Blast Resistance Genes in Main Rice Varieties in Menghai County of Yunnan Province

doi:10.11924/j.issn.1000-6850.casb2023-0524

Abstract

Abstract:

The purpose of this study is to clarify the composition of the cloned blast resistance genes Pi2, Pi9, Piz-t, Pi50/Pigm, Pita-2, and Pib among the major cultivated rice cultivars in Menghai County. Eighteen major cultivated rice cultivars grown in Menghai County were selected as experimental materials, and molecular detection was carried out using gene-specific molecular markers for the aforementioned 6 resistance genes. The results showed that the distribution frequency of the resistance gene Pib was the highest, with a detection rate of 77.78% among these tested cultivars. The detection rates of Pi2, Pita-2, and Piz-t were 22.22%, 22.22%, and 11.11%, respectively. Resistance genes Pi9 and Pi50/Pigm were not detected in the tested cultivars. Among the tested cultivars, there were 8 varieties (each accounting for 44.44 %) with 2 resistance genes and 1 resistance gene detected. There was no any one of 6 known resistance genes was detected in 2 cultivars, which was accounting for 11.11% of all tested cultivars. The results indicate that the genetic background of resistance in major rice cultivars cultivated in Menghai County is relatively narrow. It is necessary to introduce new and effective resources of resistance to broaden the resistance spectrum of rice cultivars to rice blast.

Key words: rice blast, resistance genes, molecular markers, Menghai County, molecular detection, genetic background

TIAN Weikui, DONG Liying, ZI Hongqiang, LIU Shufang, LIU Pei, YANG Qinzhong. Distribution of Six Rice Blast Resistance Genes in Main Rice Varieties in Menghai County of Yunnan Province[J]. Chinese Agricultural Science Bulletin, 2024, 40(20): 130-134.

Figures/Tables 3

References 25

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抗性基因	引物名称	引物序列(5'-3')	片段大小/bp	限制性内切酶	退火温度/℃
Pi2	Pi2SP1-FW	GCTGCAGGAATCTCAGATGG	394^a/-^b		63
	Pi2SP1-RV	CAGATTCCAACCTGCACTCG
Pi9	Pi9SPFW	TCTTCGTTGTAGTAGAAAGTTTGT	788/-		62
	Pi9SPRV	ATTCCAACCTGCAGCAAGAG
Piz-t	PiztSP-FW	GCTGTGTTATTCTCAGGTATTATT	150/-		61.4
	PiztSP-RV	GTAGATCTTCTTCAGGTGCA
Pi50/Pigm	Pi50/gmSP-FW	CTGAAGAAGTTCTACTTAC	335/81+254	AvaⅡ	50
	Pi50/gmSP-RV	AGCCCAGCCACTTTACTTCC
Pita-2	Pita2InDel-FW	TGACCTTCGCAGACCTCATC	117/129		58
	Pita2InDel-RV2	ATTTCGCAGGCATAAACTCG
Pib	Pibdom-FW	GAACAATGCCCAAACTTGAGA	365/-		58
	Pibdom-RV	GGGTCCACATGTCAGTGAGC

抗性基因	引物名称	引物序列(5'-3')	片段大小/bp	限制性内切酶	退火温度/℃
Pi2	Pi2SP1-FW	GCTGCAGGAATCTCAGATGG	394^a/-^b		63
	Pi2SP1-RV	CAGATTCCAACCTGCACTCG
Pi9	Pi9SPFW	TCTTCGTTGTAGTAGAAAGTTTGT	788/-		62
	Pi9SPRV	ATTCCAACCTGCAGCAAGAG
Piz-t	PiztSP-FW	GCTGTGTTATTCTCAGGTATTATT	150/-		61.4
	PiztSP-RV	GTAGATCTTCTTCAGGTGCA
Pi50/Pigm	Pi50/gmSP-FW	CTGAAGAAGTTCTACTTAC	335/81+254	AvaⅡ	50
	Pi50/gmSP-RV	AGCCCAGCCACTTTACTTCC
Pita-2	Pita2InDel-FW	TGACCTTCGCAGACCTCATC	117/129		58
	Pita2InDel-RV2	ATTTCGCAGGCATAAACTCG
Pib	Pibdom-FW	GAACAATGCCCAAACTTGAGA	365/-		58
	Pibdom-RV	GGGTCCACATGTCAGTGAGC

序号	品种名称	亚种	稻谷类型	抗性基因						总计
序号	品种名称	亚种	稻谷类型	Pi2	Pi9	Piz-t	Pi50/Pigm	Pib	Pita-2	总计
1	德糯2号	籼型	糯稻	-	-	+	-	+	-	2
2	文富7号	籼型	杂交稻	-	-	-	-	+	+	2
3	宜优673	籼型	杂交稻	-	-	-	-	+	+	2
4	宜香1108	籼型	杂交稻	-	-	-	-	+	-	1
5	野香优明月丝苗	籼型	杂交稻	-	-	-	-	+	+	2
6	宜香优2115	籼型	杂交稻	+	-	-	-	+	-	2
7	滇屯502	籼型	杂交稻	+	-	-	-	+	-	2
8	沪优164	籼型	杂交稻	+	-	-	-	-	-	1
9	宜香3003	籼型	杂交稻	-	-	-	-	+	-	1
10	野香油丝	籼型	杂交稻	-	-	-	-	+	-	1
11	文稻8号	籼型	常规稻	-	-	-	-	+	-	1
12	宜香725	籼型	杂交稻	-	-	-	-	-	-	0
13	糯稻	籼型	地方品种	-	-	-	-	-	-	0
14	德香4103	籼型	杂交稻	-	-	-	-	+	-	1
15	隆两优1377	籼型	杂交稻	-	-	-	-	+	-	1
17	糯稻18号	籼型	常规稻	-	-	-	-	+	-	1
16	金龙优548	籼型	杂交稻	+	-	-	-	-	+	2
18	宜香305	籼型	杂交稻	-	-	+	-	+	-	2
19	阳性对照品种	籼稻/粳稻	常规稻	+	+	+	+	+	+	-

序号	品种名称	亚种	稻谷类型	抗性基因						总计
序号	品种名称	亚种	稻谷类型	Pi2	Pi9	Piz-t	Pi50/Pigm	Pib	Pita-2	总计
1	德糯2号	籼型	糯稻	-	-	+	-	+	-	2
2	文富7号	籼型	杂交稻	-	-	-	-	+	+	2
3	宜优673	籼型	杂交稻	-	-	-	-	+	+	2
4	宜香1108	籼型	杂交稻	-	-	-	-	+	-	1
5	野香优明月丝苗	籼型	杂交稻	-	-	-	-	+	+	2
6	宜香优2115	籼型	杂交稻	+	-	-	-	+	-	2
7	滇屯502	籼型	杂交稻	+	-	-	-	+	-	2
8	沪优164	籼型	杂交稻	+	-	-	-	-	-	1
9	宜香3003	籼型	杂交稻	-	-	-	-	+	-	1
10	野香油丝	籼型	杂交稻	-	-	-	-	+	-	1
11	文稻8号	籼型	常规稻	-	-	-	-	+	-	1
12	宜香725	籼型	杂交稻	-	-	-	-	-	-	0
13	糯稻	籼型	地方品种	-	-	-	-	-	-	0
14	德香4103	籼型	杂交稻	-	-	-	-	+	-	1
15	隆两优1377	籼型	杂交稻	-	-	-	-	+	-	1
17	糯稻18号	籼型	常规稻	-	-	-	-	+	-	1
16	金龙优548	籼型	杂交稻	+	-	-	-	-	+	2
18	宜香305	籼型	杂交稻	-	-	+	-	+	-	2
19	阳性对照品种	籼稻/粳稻	常规稻	+	+	+	+	+	+	-