Study on Difference of Seedling Growth and Physiological Characteristics Between Three-way Cross Rapeseed and Its Parents Under Different Water Stress

doi:10.11924/j.issn.1000-6850.casb2023-0679

Abstract

Abstract:

Drought and flood disasters have a significant impact on rapeseed production, seriously affecting its yield and quality. This study used a pot experiment to study the effects of normal water content (control), mild waterlogging, moderate waterlogging, moderate drought, and severe drought conditions on growth and physiological characteristics of the three-way cross rapeseed ‘159-6’×‘Fengyou 520’, its parent ‘Fengyou 520’ (20A×C3R) and‘159-6’. The results showed that under normal water conditions, the fresh weight of three-way cross rapeseed was higher than that of hybrid parents and inbred line parents, and CAT activity was higher than that of inbred line parents. The dry weight, chlorophyll content, SOD and POD activity were significantly lower than those of both parents (p<0.01). Under different degrees of waterlogging and drought stress, the chlorophyll content of three-way cross rapeseed was higher than that of the control, and significantly higher than that of its parents (p<0.05). The dry weight of three-way cross rapeseed under mild waterlogging and severe drought stress was 80% and 20% higher than that of its hybrid parents, and 200% and 100% higher than that of its inbred parents, reaching a highly significant level (p<0.001). Under mild waterlogging and moderate drought stress, the SOD activity was 44.8% and 62.1% higher than the hybrid parents, and 179.9% and 48.7% higher than the inbred parents, reaching a highly significant level (p<0.001). The POD activity under moderate and severe drought stress was 64.1% and 22.6% higher than that of hybrid parents, 154.2% and 91.1% higher than that of inbred parents, reaching a highly significant level (p<0.001); Under severe drought stress, the soluble sugar content was 46.9% higher than that of the hybrid parent, reaching a significant level (p<0.05). Three-way cross rapeseed has better waterlogging resistance and drought resistance compared to its parents. Three-way cross rapeseed has significant advantages over its parents under different water stress. This study can provide reference for the breeding and large-scale promotion of new varieties of waterlogging and drought tolerant rapeseed.

Key words: three-way cross rapeseed, waterlogging, drought, superoxide dismutase

PENG Duozi, DAI Yue, ZHANG Qi, CHEN Hao, YANG Liu, ZHANG Zhenqian. Study on Difference of Seedling Growth and Physiological Characteristics Between Three-way Cross Rapeseed and Its Parents Under Different Water Stress[J]. Chinese Agricultural Science Bulletin, 2024, 40(20): 20-26.

Figures/Tables 9

References 22

[1]	李震, 吴北京, 陆光远, 等. 不同基因型油菜对苗期水分胁迫的生理响应[J]. 中国油料作物学报, 2012, 34(1):33-39.
[2]	邢君, 费俊杰, 杨建群, 等. 安徽省油菜主要气象灾害与防御技术对策[J]. 安徽农学通报, 2004(4):28-46.
[3]	陈娟妮, 梁颖. 长江流域主要甘蓝型油菜品种苗期耐湿性鉴定[J]. 中国生态农业学报, 2011, 19(3):626-630.
[4]	杨海云, 艾雪莹, BATOOL M, 等. 油菜响应水分胁迫的生理机制及栽培调控措施研究进展[J]. 华中农业大学学报, 2021, 40(2):6-16.
[5]	李玲, 张春雷, 张树杰, 等. 渍水对冬油菜苗期生长及生理的影响[J]. 中国油料作物学报, 2011, 33(3):247-252.
[6]	白鹏, 冉春艳, 谢小玉. 干旱胁迫对油菜蕾薹期生理特性及农艺性状的影响[J]. 中国农业科学, 2014, 47(18):3566-3576. doi: 10.3864/j.issn.0578-1752.2014.18.005
[7]	朱小慧, 马君红, 刘锋博, 等. 干旱胁迫下甘蓝型油菜幼苗萌发特性及生理指标分析[J]. 西北农业学报, 2021, 30(9):1331-1337.
[8]	洪双, 李浩, 许鲲, 等. 甘蓝型油菜微核心种质耐旱鉴定与评价指标筛选[J]. 中国油料作物学报, 2018, 40(2):209-217. doi: 10.7505/j.issn.1007-9084.2018.02.006
[9]	庞红喜, 赵兰芝. 干旱胁迫下不同油菜新品种萌发期耐旱性的比较[J]. 安徽农业科学, 2016, 44(19):38-41.
[10]	王琼, 张春雷, 李光明, 等. 渍水胁迫对油菜根系形态与生理活性的影响[J]. 中国油料作物学报, 2012, 34(2):157-162.
[11]	张树杰, 廖星, 胡小加, 等. 渍水对油菜苗期生长及养分吸收的影响[J]. 中国油料作物学报, 2013, 35(6):650-657. doi: 10.7505/j.issn.1007-9084.2013.06.006
[12]	张学昆, 范其新, 陈洁, 等. 不同耐湿基因型甘蓝型油菜苗期对缺氧胁迫的生理差异响应[J]. 中国农业科学, 2007(3):485-491.
[13]	张璞, 李殿荣, 田建华. 甘蓝型油菜隐性核不育系三交种的杂种优势分析[J]. 西北农业学报, 2007, 16(5):89-91.
[14]	耿博, 傅廷栋. 油菜三交种与单交种优势比较[J]. 河南农业科学, 2008(6):51-53. doi: 10.3969/j.issn.1004-3268.2008.06.013
[15]	萧浪涛, 王三根. 植物生理学试验技术[M]. 北京: 中国农业出版社, 2005:211-215.
[16]	ZHANG S J, LI L, ZHANG C L, et al. Influences of cadmium on the growth and micro-elements contents of oilseed rape seedlings[J]. Journal of agro-environment science, 2011, 30(5):836-842.
[17]	张树杰, 廖星, 胡小加, 等. 渍水对油菜苗期生长及生理特性的影响[J]. 生态学报, 2013, 33(23):7382-7389.
[18]	李素. 三种类型油菜典型品种对干旱胁迫的生理响应[D]. 北京: 中国农业科学院, 2020.
[19]	时振振, 李胜, 马绍英, 等. 不同品种小麦抗氧化系统对水分胁迫的响应[J]. 草业学报, 2015, 24(7):68-78. doi: 10.11686/cyxb2014342
[20]	马海清, 刘清云, 高立兵, 等. 油菜初花期淹水胁迫对产量及构成因子的影响[J]. 中国农业文摘-农业工程, 2020, 32(6):77-80.
[21]	ZHANG S J, HU F, LI H X, et al. Influence of earthworm mucus and amino acids on tomato seedling growth and cadmium accumulation[J]. Environmental pollution, 2009, 157:2737-2742. doi: 10.1016/j.envpol.2009.04.027 pmid: 19535186
[22]	赵咏梅. 植物SOD在抵抗干旱胁迫中的作用[J]. 生物学教学, 2011, 36(3):4-5.