Effect of Exogenous Strigolactones on Seed Germination of Cosmos bipinnatus

doi:10.11924/j.issn.1000-6850.casb2024-0647

Abstract

Abstract:

In order to explore the effects of exogenous strigolactones (rac-GR24) on Cosmos bipinnatus seed germination, the seeds were treated with different concentrations (0, 0.1, 0.5, 1, 2, 5 μmol/L) of rac-GR24, and the effects of treatments on seed germination, morphological indicators and physiological characteristics were determined and analyzed. The results showed that compared with the control (0 μmol/L), the germination rates increased first and decreased afterward with different concentrations of rac-GR24 every day in the first three days, and reached the highest points in 1 μmol/L rac-GR24 treatment, and had no significantly difference on the 4^th and 5^th day. With the increase of rac-GR24 concentrations, fresh weight of aboveground parts in each treatment showed a decreasing trend, while fresh weight of underground parts and dry weight of aboveground parts both showed a trend of decreasing first and increasing afterward, however, dry weight of underground parts had no significantly difference, the length of aboveground and underground parts both showed a decreasing trend. The activities of α-amylase and β-amylase, contents of soluble starch and total soluble sugar were significantly increased at 18 h under applying exogenous 1 μmol/L rac-GR24 treatment, meanwhile ABA content was decreased, cZR content and GA₂₉ content were increased, however IAA content had no significant change. The results indicated that exogenous SLs (rac-GR24) could stimulate the seeds germination of C. bipinnatus, and provided a theoretical basis for the mechanism and application of plant growth regulators in regulation of seed germination.

Key words: Cosmos bipinnatus, strigolactones, germination, physiological characteristics, enzymatic activity, phytohormones, plant growth regulator

GUAN Yuxi, WEN Chao. Effect of Exogenous Strigolactones on Seed Germination of Cosmos bipinnatus[J]. Chinese Agricultural Science Bulletin, 2025, 41(16): 40-44.

Figures/Tables 4

References 26

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处理	地上部鲜重/g	地下部鲜重/g	地上部干重/g	地下部干重/g	地上部长度/cm	地下部长度/cm
CK	0.905±0.003a	0.135±0.006a	0.062±0.001ab	0.011±0.001a	4.863±0.022a	3.773±0.163a
0.1 μmol/L rac-GR24	0.899±0.022a	0.141±0.011a	0.061±0.001ab	0.012±0.001a	4.749±0.072a	3.787±0.172a
0.5 μmol/L rac-GR24	0.794±0.012b	0.115±0.002b	0.056±0.001c	0.010±0.001a	4.608±0.064a	3.638±0.162ab
1 μmol/L rac-GR24	0.802±0.058b	0.119±0.006b	0.059±0.002bc	0.010±0.001a	4.687±0.335a	3.535±0.418ab
2 μmol/L rac-GR24	0.811±0.018b	0.118±0.003b	0.057±0.001bc	0.011±0.001a	4.448±0.176ab	3.757±0.029a
5 μmol/L rac-GR24	0.803±0.038b	0.126±0.079ab	0.065±0.003a	0.010±0.001a	4.162±0.339b	3.251±0.055b

处理	地上部鲜重/g	地下部鲜重/g	地上部干重/g	地下部干重/g	地上部长度/cm	地下部长度/cm
CK	0.905±0.003a	0.135±0.006a	0.062±0.001ab	0.011±0.001a	4.863±0.022a	3.773±0.163a
0.1 μmol/L rac-GR24	0.899±0.022a	0.141±0.011a	0.061±0.001ab	0.012±0.001a	4.749±0.072a	3.787±0.172a
0.5 μmol/L rac-GR24	0.794±0.012b	0.115±0.002b	0.056±0.001c	0.010±0.001a	4.608±0.064a	3.638±0.162ab
1 μmol/L rac-GR24	0.802±0.058b	0.119±0.006b	0.059±0.002bc	0.010±0.001a	4.687±0.335a	3.535±0.418ab
2 μmol/L rac-GR24	0.811±0.018b	0.118±0.003b	0.057±0.001bc	0.011±0.001a	4.448±0.176ab	3.757±0.029a
5 μmol/L rac-GR24	0.803±0.038b	0.126±0.079ab	0.065±0.003a	0.010±0.001a	4.162±0.339b	3.251±0.055b

处理	IAA	ABA	cZR	GA₂₉
CK	31.38±0.21a	9.47±1.71a	0.97±0.06b	22.14±2.22b
1 μmol/L rac-GR24	30.37±1.53a	7.66±0.50b	1.34±0.10a	35.12±1.71a

处理	IAA	ABA	cZR	GA₂₉
CK	31.38±0.21a	9.47±1.71a	0.97±0.06b	22.14±2.22b
1 μmol/L rac-GR24	30.37±1.53a	7.66±0.50b	1.34±0.10a	35.12±1.71a