Abstract:

In order to accelerate propagation of elite variety of Arundo donax ‘Hangyu No.1’, and establish an efficient regeneration system, the stem segments of 3-year-old plants were used as explants to systematically explore the effects of sampling time and plant growth regulator combinations on the regeneration system. The results revealed that the best sampling time was in July and August, and the field plants were in a rapid growth period. The explant sterilization used 75% ethanol for 30 seconds and then 0.1% HgCl₂ for 15-20 min, resulting in a survival rate of 90% approximately. The medium for axillary bud induction was MS+ 6-BA 4 mg/L+ IBA 1 mg/L, 92.66% buds developed obviously. Bud proliferation medium was MS+ 6-BA 7 mg/L+IBA 1 mg/L, resulting in a proliferation coefficient of 3.51. The rooting medium was 1/2MS+ NAA 0.2 mg/L, and the rooting rate reached 97.36%. The best time for plant transfer to ex vitro conditions was 7-10 days after rooting. The survival rate was 92.22% in all the transferred plants. The plants were further moved to the field at the end of April and then under the standard field management. In November of the same year, the plant grew up to 3-3.5 m high with 30-50 tillers. This research provides solid technical support for large-scale production of ‘Hangyu No.1’.

Key words: Arundo donax, ‘Hangyu No.1’, tissue culture, efficient propagation system, stem, plant growth regulator, large-scale production