Abstract:

To investigate the soil bacterial community structure of the Toona sinensis cultivation area in Taihe, soil samples from the rhizosphere of Toona sinensis from Taihe were collected. The 16S rDNA V3-V4 region of soil bacteria was sequenced using high-throughput sequencing technology on the Illumina HiSeq platform, and bioinformatics analysis was performed based on the data. The results showed that a total of 1628 operational taxonomic units (OTUs) were obtained, belonging to 26 phyla, 63 classes, 149 orders, 261 families, 512 genera and 574 species. At the phylum level, the dominant bacteria in the rhizosphere soil of Toona sinensis were Proteobacteria (24.26%), Actinobacteria (22.97%) and Firmicutes (21.50%). COG and KEGG annotation analysis of microbiota gene function showed that rhizosphere soil colonies of Toona sinensis could synthesize natural products, such as terpenoids, type II polyketones, aminoglycosides, β-lactams, steroids, non-ribosomal peptides, etc. These results indicated that the bacteria in the rhizosphere soil of Toona sinensis in Taihe not only had rich diversity but also could produce structure-rich and active natural products. The bacteria in the rhizosphere soil of Toona sinensis had great potential for discovering new active natural compounds. The results of this study are of great significance for the utilization and development of microbial resources in the rhizosphere soil of Toona sinensis.

Key words: Toona sinensis cultivated in Taihe, Illumina HiSeq, rhizosphere soil, bacterial diversity, natural products