Effects of Straw Returning Combined with Nitrogen Fertilizer on Paddy Soil Bacterial Community Diversity in Central South Region of China

doi:10.11924/j.issn.1000-6850.casb16090007

Abstract

Abstract: The aim is to study the effects of straw returning combined with nitrogen fertilizer on paddy soil bacterial community diversity. Different treatments in field were set as following: gradient amount of nitrogen fertilizer (U), straw returning combined with gradient amount of nitrogen fertilizer (SU), no straw return and no nitrogen fertilizer as the control. The soil bacterial community structure, diversity index and richness index of different treatments in different rice growing stages were analyzed and compared by using PCR-DGGE cloning and sequencing technology. The results showed that the diversity index and richness index of SU treatment were higher than that of U treatment at seedling stage, tillering stage and elongation stage. On the contrast, these indexes of SU treatment were lower than that of U treatment at filling stage and maturation stage. With the nitrogen fertilizer increasing, the diversity index and richness index of SU and U treatments showed different trends, however the evenness index showed no significant variation. Meanwhile, through DGGE fingerprint analysis, the specific bands appeared at the tillering stage, filling stage and maturity stage were mainly identified as Sphingomonas belonging to Proteobacteria, Streptomyces sp. belonging to Actinobacteria, Comamonadaceae belonging to Betaproteobacteria, Sphingomonadaceae belonging to Alphaproteobacteria, Acidobacteria, Methylomonadaceae, Epsilonproteobacteria, Nitrospira and Firmicutes, etc.

CLC Number:

Q938.1

References

[1] 高豹华,郭春莲.农作物秸秆资源的综合利用[J].科学导报,2013,(14):327-327,328.
[2] 刘义国,林琪,王宁.秸秆还田与氮供应对旱地麦田土壤养分状况及土壤呼吸的影响[C]中国作物学会2013年学术年会论文摘要集. 2013.
[3] 毛红玲,李军,贾志宽,等.旱作麦田保护性耕作蓄水保墒和增产增收效应[J].农业工程学报, 2010,26(8):44-51.
[4] 丁文侠.大豆田间覆盖麦秸增产效果及技术要点[J].安徽农学通报, 2012,18(19):60-61.
[5] 张水清,钟旭华,黄绍敏等.中国稻草还田技术研究进展[J].中国农学通报,2010,26(15):332-335.
[6] 霍竹,付晋锋,王璞.秸秆还田和氮肥施用对夏玉米氮肥利用率的影响[J].土壤,2005,37(2):202-204.
[7] Caballeromellado J. Agriculture microbiology and microbe interaction with plants.[J]. Revista Latinoamericana De Microbiología, 2006, 48(2):154-61.
[8] 谭益民,何苑皞,郭文平.基于分子技术的土壤微生物多样性研究进展[J].中南林业科技大学学报(自然科学版),2014,(10):1-9.
[9] Jennifer L Kirk , Lee A .Beaudette, Miranda Hart ,et al .Methods of studying soil microbial diversity[J].Journal of Microbiological Methods,2004,58:169-188 .
[10] 邢华铭,杜海涛,张黎黎,等. PCR-DGGE与Biolog技术在土壤微生物多样性研究中的比较[J].农业开发与装备, 2013,(10):48-48,68.
[11] Can S, Liping L, Yanqing D, et al. Culture-independent methods for studying environmental microorganisms: methods, application, and perspective.[J]. Applied Microbiology Biotechnology, 2011, 93(3):993-1003.
[12] 李安英,张潞生,高微微,等.适于变性梯度凝胶电泳(DGGE)分析的草莓根际土壤微生物的 DNA 模板制备[J].农业生物技术学报,2009,17(4):701-706 .
[13] Ulrich A , Becker R . Soil parent material is a key determinant of the bacterial community structure in arable soils. FEMS Microbiology Ecology,2006,56(3) :43-44
[14] 郭梨锦,曹凑贵,张枝盛,等.耕作方式和秸秆还田对稻田表层土壤微生物群落的短期影响[J]. 农业环境科学学报,2013,32(8):1577-1584.
[15] 萨如拉,高聚林,青格尔,等. 玉米秸秆深翻还田土壤细菌群落16SrDNA-PCR-DGGE分析[J]. 玉米科学,2014,22(3):43-47 53.
[16] 王霞,陈哲,袁红朝,等. 应用16S rDNA克隆文库技术研究长期稻草还田对水稻土细菌多样性的影响[J]. 生态学报,2010,30(14):3865-3874.
[17] Janssen, P. H. (2006).Identifying the dominant soil bacterial taxa in libraries of 16S rRNA and 16S rRNA genes.Appl Environ Microbiol72,1719-1728.
[18] Jones, R. T., Robeson, M. S., Lauber, C. L., Hamady, M., Knight, R. Fierer, N. (2009).A comprehensive survey of soil acidobacterial diversity using pyrosequencing and clone library analyses.ISME J3,442–453.
[19] Takai, Ken; et al. (2005). Enzymatic and genetic characterization of carbon and energy metabolisms by deep-sea hydrothermal chemolithoautotrophic isolates of Epsilonproteobacteria. Applied and Environmental Microbiology 71(11): 7310-7320. doi:10.1128/aem.71.11.7310-7320.2005.
[20] Campbell, Barbara J.; Annette Summers Engel; Megan L. Porter; Ken Takai (2006-05-02). The versatile ε-proteobacteria: key players in sulphidic habitats. Nature Reviews Microbiology 4(6): 458–468.doi:10.1038/nrmicro1414. ISSN 1740-1526. Retrieved 2012-11-09.
[21] Burrell P,KellerJ,BlackallL.Characterisation of the bacterial consortium involved in nitrite oxidation in avtivatedsludge.Water Science and Technology,1999,39(6):45-52.
[22] Lee H W,Lee S Y,Lee J B,Park J B,Choi E S,Park Y K,Molecularcharaterization of microbial community in nitrate-removing activated sludge.FEMS Microbiology Ecology,2002,41(2):85-94
[23] Liebig MA, Varvel GE, DoranJW. Crop sequence and nitrogen fertilization effects on soil properties In the Western Corn Belt[J].Soil SciSoc Am J, 2002, (66): 596-601.
[24] 王小芬,杨洪岩,马静静等.连年秸杆还田地土壤理化性状及微生物群特性[N].第十次全国环境微生物学术研讨会论文摘要集,2007 (26).
[25] 袁红朝,秦红灵,刘守龙,等. 长期施肥对红壤性水稻土细菌群落结构和数量的影响[J]. 中国农业科学,2011,42(22):4610-4617.
[26] 李自刚,李兴道,蒋媛媛,等.水稻秸秆还田对河南沿黄稻区土壤细菌群落分子多态性影响[J].河南农业大学学报,2008, 42(1): 90-94.
[27] Dey R, Pal K K, Tilak K V B R. Influence of soil and plant types ondiversity of rhizobacteria[J]. Proceedings of the National Academy of Sciences, 2012, 82(3): 341-352.