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Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (18): 100-103.doi: 10.11924/j.issn.1000-6850.casb16010155

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Endophytic Bacteria from Schisandra chinensis

Cao Cong, Liang Kangkang, Xu Lijian, Shao Hongtao, Wang Jian, Li Yafei, Bi Zexing, Wang Qinggui   

  1. (College of Agricultural Resource and Environment, Heilongjiang University, Harbin 150080)
  • Received:2016-01-31 Revised:2016-03-17 Accepted:2016-03-22 Online:2016-06-28 Published:2016-06-28

Abstract: The resource of Schisandra chinesis is rich in China. In order to investigate the composition and biodiversity of endophytic bacteria from Schisandra chinesis, the bacterial rDNA was amplified from total DNA, and then the composition and biodiversity of the endophytic bacteria from Schisandra chinesis were analyzed by pyrosequencing MiSeq method. It was found that the endophytic bacteria belonged to 6 phyla, 9 classes, 17 orders, 27 families, 31 genera and 39 species, respectively. Cyanobacteria were the dominant bacteria and its content was approximately 88.19%. Except for Cyanobacteria, Enterococcus, Lactococcus and Bacillus, three genera, represented 4.79%, 2.59%, 1.11% of the total bacteria. Other bacteria accounted for 3.32% of the total. With respect to the biodiversity of bacteria, Shannon index was 0.633, Chao index was 39 and Simpson index was 0.781. The composition and biodiversity of endophytic bacteria from Schisandra chinensis were investigated by using high-throughput sequencing method. It was found that the biodiversity of the endophytic bacteria from the fruit of Schisandra chinensis was lower than that of the endophytic bacteria from other plants.

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