Preparation and Characterization of Alginate Polysaccharide-Selenium Nanoparticles Complex

doi:10.11924/j.issn.1000-6850.casb2024-0693

Abstract

Abstract:

The study aimed to optimize the preparation process of alginate polysaccharide-selenium nanoparticles (APS-SeNPs) for developing efficient and stable nano-selenium fertilizers. The combination experiment of orthogonal and single-factor was designed to prepare APS-SeNPs. The ratio of absorbance at 410 nm to 490 nm (A₄₁₀/A₄₉₀) served as a critical indicator for evaluating particle size and stability of APS-SeNPs. Morphological structure and element distribution characterization of APS-SeNPs were performed using scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS). The research results indicated that the optimal preparation conditions of APS-SeNPs were determined as follows. APS concentration of 1200 mg/L, molar ratio of vitamin C (Vc) to sodium selenite (Na₂SeO₃) at 4:1, reaction temperature of 45℃, and reaction time of 1.5 h. The prepared APS-SeNPs exhibited uniformly dispersed spherical structure which particle size was (91.30±7.20) nm, with A₄₁₀/A₄₉₀ ratio of 1.96±0.01 and selenium (Se) loading capacity of 31.51%. Energy spectrum analysis revealed that the proportion of carbon and oxygen elements in APS-SeNPs increased by 38.97% and 336.94%, respectively, compared with selenium nanoparticles (SeNPs) unmodified by alginate polysaccharide, indicating that alginate polysaccharide modified the surface of selenium nanoparticles effectively. The APS-SeNPs prepared by this optimized process possessed fine stability and Se loading, providing a theoretical basis for the development of novel nano-selenium fertilizers.

Key words: selenium nanoparticles, alginate polysaccharide, preparation, scanning electron microscopy, energy spectrum analysis, dual-wavelength spectrophotometry (A₄₁₀/A₄₉₀), orthogonal experimental design, single-factor experiment

JIN Dandan, HUANG Zhenghua, CHANG Xiaoyu, ZHANG Kangkang, WEI Yihua, ZHANG Biaojin. Preparation and Characterization of Alginate Polysaccharide-Selenium Nanoparticles Complex[J]. Chinese Agricultural Science Bulletin, 2025, 41(22): 27-34.

Figures/Tables 7

References 25

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水平	因素
水平	A.海藻多糖浓度/(mg/L)	B.反应温度/℃	C.反应时间/h	D.n(维生素C):n(Na₂SeO₃)
1	600	30	0.5	2:1
2	800	35	1	4:1
3	1000	40	1.5	6:1
4	1200	45	2	10:1
5	1400	50	2.5	12:1

水平	因素
水平	A.海藻多糖浓度/(mg/L)	B.反应温度/℃	C.反应时间/h	D.n(维生素C):n(Na₂SeO₃)
1	600	30	0.5	2:1
2	800	35	1	4:1
3	1000	40	1.5	6:1
4	1200	45	2	10:1
5	1400	50	2.5	12:1

编号	因素				A₄₁₀/A₄₉₀
编号	A.海藻多糖浓度	B.反应温度	C.反应时间	D.n(维生素C):n(Na₂SeO₃)	A₄₁₀/A₄₉₀
1	1	1	1	1	1.734±0.024
2	1	2	2	2	1.771±0.057
3	1	3	3	3	1.033±0.083
4	1	4	4	4	0.950±0.004
5	1	5	5	5	0.965±0.005
6	2	1	2	3	1.460±0.327
7	2	2	3	4	1.005±0.056
8	2	3	4	5	1.013±0.043
9	2	4	5	1	1.021±0.047
10	2	5	1	2	1.905±0.044
11	3	1	3	5	1.066±0.093
12	3	2	4	1	1.867±0.018
13	3	3	5	2	1.840±0.020
14	3	4	1	3	1.821±0.102
15	3	5	2	4	1.196±0.213
16	4	1	4	2	1.932±0.016
17	4	2	5	3	1.803±0.107
18	4	3	1	4	1.629±0.107
19	4	4	2	5	1.033±0.025
20	4	5	3	1	1.912±0.018
21	5	1	5	4	1.449±0.061
22	5	2	1	5	1.342±0.188
23	5	3	2	1	1.817±0.011
24	5	4	3	2	1.957±0.003
25	5	5	4	3	1.899±0.024

编号	因素				A₄₁₀/A₄₉₀
编号	A.海藻多糖浓度	B.反应温度	C.反应时间	D.n(维生素C):n(Na₂SeO₃)	A₄₁₀/A₄₉₀
1	1	1	1	1	1.734±0.024
2	1	2	2	2	1.771±0.057
3	1	3	3	3	1.033±0.083
4	1	4	4	4	0.950±0.004
5	1	5	5	5	0.965±0.005
6	2	1	2	3	1.460±0.327
7	2	2	3	4	1.005±0.056
8	2	3	4	5	1.013±0.043
9	2	4	5	1	1.021±0.047
10	2	5	1	2	1.905±0.044
11	3	1	3	5	1.066±0.093
12	3	2	4	1	1.867±0.018
13	3	3	5	2	1.840±0.020
14	3	4	1	3	1.821±0.102
15	3	5	2	4	1.196±0.213
16	4	1	4	2	1.932±0.016
17	4	2	5	3	1.803±0.107
18	4	3	1	4	1.629±0.107
19	4	4	2	5	1.033±0.025
20	4	5	3	1	1.912±0.018
21	5	1	5	4	1.449±0.061
22	5	2	1	5	1.342±0.188
23	5	3	2	1	1.817±0.011
24	5	4	3	2	1.957±0.003
25	5	5	4	3	1.899±0.024

方差来源	III类平方和	自由度	均方	F值	显著性
修正模型	3.342	16	0.209	5.916	0.008
截距	56.007	1	56.007	1586.292	0.000
A	0.793	4	0.198	5.617	0.019
B	0.157	4	0.039	1.114	0.414
C	0.279	4	0.070	1.979	0.191
D	2.112	4	0.528	14.955	0.001
误差	0.282	8	0.035
总计	59.632	25
修正后总计	3.625	24