Comparative Analysis of Antioxidant Capacity of Four Natural Plant Extracts in vitro

doi:10.11924/j.issn.1000-6850.casb2025-0113

Abstract

Abstract:

This study aims to compare the in vitro antioxidant capacities of four commercially available natural plant extracts, namely isochlorogenic acid, tannic acid, curcumin, and β-carotene, and to clarify their antioxidant activities and effectiveness. Using above four natural extracts as test materials, antioxidant-related indicators were evaluated through free radical scavenging assays, reduction capacity measurements and a red blood cell oxidative stress model. The test results showed that the DPPH radical scavenging rates were 60.91% for isochlorogenic acid, 82.87% for tannic acid, 65.11% for curcumin, and 6.23% for β-carotene when the concentration of natural plant extract was 100 μg / mL. The FRAP value of isochlorogenic acid was the highest, reaching 1096.89 μmol Fe²⁺/mL. In the red blood cell oxidative stress model, compared with the AAPH model group, the hemolysis rates in the isochlorogenic acid, tannic acid, and curcumin groups were significantly decreased by 42.66% (P<0.05), 68.48% (P<0.05), and 33.29% (P<0.05), respectively; and the MDA contents were significantly decreased by 52.88% (P<0.05), 60.90% (P<0.05), 47.28% (P<0.05), and 4.01% (P<0.05), respectively. The SOD activity in the tannic acid group was significantly increased by 45.09% (P<0.05), while the isochlorogenic acid, curcumin, and β-carotene groups were increased by 8.41% (P>0.05), 3.98% (P>0.05), and 6.65% (P>0.05), respectively, with no significant differences. In summary, all four natural plant extracts showed good in vitro antioxidant activities, and the order of their antioxidant capacities from strong to weak was: tannic acid > isochlorogenic acid > curcumin > β-carotene.

Key words: isochlorogenic acid, tannic acid, curcumin, β-carotene, free radical scavenging, antioxidant

AN Xiaomin, LIANG Ziyi, WU Lifei, YUAN Yuting, XIA Linzhi, WU Guojiang, LIU Jie, HAO Qinghong, GUO Yunxia. Comparative Analysis of Antioxidant Capacity of Four Natural Plant Extracts in vitro[J]. Chinese Agricultural Science Bulletin, 2025, 41(29): 113-121.

Figures/Tables 9

References 49

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名称	规格	来源
异绿原酸	纯度60%	晨光生物科技股份有限公司
单宁酸	纯度98%	山东科源生化有限公司
姜黄素	纯度99%	山东科源生化有限公司
β-胡萝卜素	纯度96%	山东科源生化有限公司

名称	规格	来源
异绿原酸	纯度60%	晨光生物科技股份有限公司
单宁酸	纯度98%	山东科源生化有限公司
姜黄素	纯度99%	山东科源生化有限公司
β-胡萝卜素	纯度96%	山东科源生化有限公司

浓度/(μg/mL)	异绿原酸	单宁酸	姜黄素	β-胡萝卜素	均值标准误	P值
5	22.45b	48.00a	24.03b	15.79b	13.563	<0.001
10	28.83b	49.03a	25.30b	15.15b	13.381	<0.001
20	39.49b	78.9a	43.97b	13.16b	24.814	<0.001
40	54.09b	77.84a	49.08b	11.36c	25.876	<0.001
80	55.81b	81.59a	52.20b	10.59b	27.455	<0.001
100	60.91b	82.87a	65.11a	6.23c	30.224	<0.001
SEM	16.815	12.628	15.304	3.539
P值	0.001	<0.001	<0.001	<0.001

浓度/(μg/mL)	异绿原酸	单宁酸	姜黄素	β-胡萝卜素	均值标准误	P值
5	22.45b	48.00a	24.03b	15.79b	13.563	<0.001
10	28.83b	49.03a	25.30b	15.15b	13.381	<0.001
20	39.49b	78.9a	43.97b	13.16b	24.814	<0.001
40	54.09b	77.84a	49.08b	11.36c	25.876	<0.001
80	55.81b	81.59a	52.20b	10.59b	27.455	<0.001
100	60.91b	82.87a	65.11a	6.23c	30.224	<0.001
SEM	16.815	12.628	15.304	3.539
P值	0.001	<0.001	<0.001	<0.001

浓度/(μg/mL)	异绿原酸	单宁酸	姜黄素	β-胡萝卜素	均值标准误	P值
5	147.78a	48.44b	21.11c	1.78d	59.056	<0.001
10	234.11a	110.33b	65.67c	7.11d	87.257	<0.001
20	533.22a	194.00b	104.00c	1.11d	208.691	<0.001
40	890.67a	350.00b	187.00c	6.00d	345.251	<0.001
80	1062.5a	605.78b	343.89c	5.70d	403.427	<0.001
100	1096.89a	744.89b	411.33c	10.10d	420.075	<0.001
SEM	391.067	263.677	148.075	5.727
P值	<0.001	<0.001	<0.001	0.426