Effects of Arbuscular Mycorrhiza Fungi Inoculated in Vitro on Growth and Development of Tree Peony Vitroplantlets

doi:10.11924/j.issn.1000-6850.casb2024-0539

Abstract

Abstract:

To explore arbuscular mycorrhiza (AM) fungi of mycorrhization process and the impact on its growth and development, with tree peony cultivar ‘Wulong Pengsheng’ for bud-micropropagated vitroplantlets of rooting seedlings and hybrid system ‘Z1-WM’ and ‘Z1-10-3’ embryo culture rooting plantlets as the research objects, different kinds of AMF spores in vitro were inoculated. The results showed that active effects of AMF on the growth of mycorrhizal vitroplantlet were investigated. Inoculation of AMF in vitro could increase the transplanting survival ratio of tree peony effectively. The treatments of inoculation of AMF in vitro heightened sprouting rate epicotyl dormancy of plantlets more than CK, and the highest was 99.50%. The time of breaking epicotyl dormancy was 30 d ahead of inoculation of AMF in vitro than that of the control. There was significant difference among inoculation of AMF in vitro and GA₃ treatment, 2-4℃ low temperature and the control. The most significant difference was in ‘Z1-WM’ and ‘Z1-10-3’ inoculated with Gg+Gm. As for ‘Wulong Pengsheng’, after transplantation of 6 months inoculated with Gg, the index was 64.93%, 70.90%, 35.00%, 54.55%, 41.15% respectively. Therefore, AM fungi had a significant effect on the growth and development of mycorrhized vitroplantlets of tree peony and shorten the breeding time.

Key words: tree peony, inoculated in vitro, AM fungi, arbuscular mycorrhiza, mycorrhized vitroplantlets, breaking epicotyl dormancy, growth and development

ZENG Duanxiang, YU Ning, DU Juan, ZENG Yao, WANG Lianying. Effects of Arbuscular Mycorrhiza Fungi Inoculated in Vitro on Growth and Development of Tree Peony Vitroplantlets[J]. Chinese Agricultural Science Bulletin, 2025, 41(7): 55-60.

Figures/Tables 6

References 33

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处理	打破上胚轴休眠所需时间/d	萌发率/%	生长状况描述
CK	≥40	19.35±0.20Dd	上胚轴基本不萌发，子叶发达，顶端圆阔
2~4℃处理5周	15~20	72.12±1.53Cc	生长较矮，健壮，叶少，株高2~3 cm
GA₃处理	7~10	87.76±1.89Bb	生长细弱，易黄化，叶数1~4叶，株高4~5 cm
接种AM真菌	5~7	99.50±2.12Aa	生长健壮，叶数4~7叶，株高8~10 cm

处理	打破上胚轴休眠所需时间/d	萌发率/%	生长状况描述
CK	≥40	19.35±0.20Dd	上胚轴基本不萌发，子叶发达，顶端圆阔
2~4℃处理5周	15~20	72.12±1.53Cc	生长较矮，健壮，叶少，株高2~3 cm
GA₃处理	7~10	87.76±1.89Bb	生长细弱，易黄化，叶数1~4叶，株高4~5 cm
接种AM真菌	5~7	99.50±2.12Aa	生长健壮，叶数4~7叶，株高8~10 cm

AM真菌种类	Z1-10-3				Z1-WM
AM真菌种类	根颈上部生长长度/cm	根颈下部生长长度/cm	真叶数/片	根数/条	根颈上部生长长度/cm	根颈下部生长长度/cm	真叶数/片	根数/条
Gg	2.2Bb	3.5Cc	2.3Cc	2.0Bb	2.8Bb	3.3Bb	2.5Aa	2.3Bb
Gm	3.5Aa	4.0Bb	3.0Bb	2.5Aa	1.6Cc	2.6Cc	1.5Bb	1.8Cc
Gg+Gm	3.3Aa	4.3Aa	5.5Aa	2.6Aa	3.5Aa	5.6Aa	2.4Aa	2.5Aa
CK	1.5Cc	2.5Dd	0Dd	1.0Cc	0.8Dd	2.3Cd	0Cc	1.2Dd

AM真菌种类	Z1-10-3				Z1-WM
AM真菌种类	根颈上部生长长度/cm	根颈下部生长长度/cm	真叶数/片	根数/条	根颈上部生长长度/cm	根颈下部生长长度/cm	真叶数/片	根数/条
Gg	2.2Bb	3.5Cc	2.3Cc	2.0Bb	2.8Bb	3.3Bb	2.5Aa	2.3Bb
Gm	3.5Aa	4.0Bb	3.0Bb	2.5Aa	1.6Cc	2.6Cc	1.5Bb	1.8Cc
Gg+Gm	3.3Aa	4.3Aa	5.5Aa	2.6Aa	3.5Aa	5.6Aa	2.4Aa	2.5Aa
CK	1.5Cc	2.5Dd	0Dd	1.0Cc	0.8Dd	2.3Cd	0Cc	1.2Dd

AM真菌种类	株高/cm	根长/cm	地上部干重/g	地下部干重/g	叶面积/cm²	菌根依赖性/%
CK	6.13±0.07Cc	7.56±0.08Dd	0.20±0.01Cd	0.22±0.03Dd	15.94±0.36Cc
Gm	8.78±0.21Bb	9.71±0.15Cc	0.25±0.01ABc	0.28±0.01Cc	18.07±0.62Bb	126.19±2.40Cc
Gg	10.11±0.19Aa	12.92±0.13Aa	0.27±0.02Ab	0.34±0.01Aa	22.50±0.48Aa	145.24±3.30Aa
Gg+Gm	9.64±0.32Aa	11.12±0.17 Bb	0.28±0.01Aa	0.31±0.02Bb	23.38±0.76Aa	140.48±2.17Bb