Effect of BvFVE1 Overexpression on Bolting and Flowering of Sugar Beet

doi:10.11924/j.issn.1000-6850.casb2025-0082

Abstract

Abstract:

To explore the regulatory role of BvFVE1 in bolting and flowering of sugar beet and to understand the molecular mechanisms of bolting and flowering in sugar beet, the subcellular localization of BvFVE1 was firstly predicted by DeepLoc and analyzed by Agrobacterium-mediated transient expression in this study. Subsequently, sugar beet cultivar KWS9147 was used as the experimental material, and the effect of transient expression of BvFVE1 on the expression of bolting and flowering genes was detected by qRT-PCR. Arabidopsis was transformed via the floral-dip method to observe the effect of heterologous expression of BvFVE1 on bolting and flowering. The DeepLoc prediction showed that the scores for nuclear and cytoplasmic localization of BvFVE1 protein were 0.527 and 0.4535, respectively. After transient expression，BvFVE1 was distributed in a punctate pattern in the nucleus and cytoplasm of leaf epidermal cells. In sugar beet cotyledons, transient expression of BvFVE1 significantly downregulated the expression levels of BvBTC1, BvFT2, BvGI, and BvFY were significantly downregulated by 2.90, 3.27, 2.91, and 2.45 fold, respectively. Heterologous expression of BvFVE1 in Arabidopsis delayed bolting and flowering by approximately 2 and 3 days, respectively. These results indicate that BvFVE1 plays an inhibitory role in bolting and flowering in sugar beet. This study provides a theoretical basis for further investigation into the molecular mechanism by which BvFVE1 regulates bolting and flowering.

Key words: sugar beet, BvFVE1, bolting, flowering, vernalization, subcellular localization, transient expression, heterologous transformation

LIU Yifei, ZHANG Chunxue, BAO Jinmei, PI Zhi. Effect of BvFVE1 Overexpression on Bolting and Flowering of Sugar Beet[J]. Chinese Agricultural Science Bulletin, 2026, 42(2): 10-15.

Figures/Tables 5

References 21

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	细胞质	细胞核	胞外	细胞膜	线粒体	质体	内质网	溶酶体/液泡	高尔基体	过氧化物酶体
阈值	0.4761	0.5014	0.6173	0.5646	0.6220	0.6395	0.6090	0.5848	0.6494	0.7364
预测值	0.4535	0.5257	0.0205	0.1621	0.0708	0.0251	0.1890	0.2153	0.1966	0.0164

	细胞质	细胞核	胞外	细胞膜	线粒体	质体	内质网	溶酶体/液泡	高尔基体	过氧化物酶体
阈值	0.4761	0.5014	0.6173	0.5646	0.6220	0.6395	0.6090	0.5848	0.6494	0.7364
预测值	0.4535	0.5257	0.0205	0.1621	0.0708	0.0251	0.1890	0.2153	0.1966	0.0164