Structure of Carbon Sequestration Bacterial Community and Screening of Carbon Sequestration Microorganisms in Rhizosphere Soil of Wine Grapes

doi:10.11924/j.issn.1000-6850.casb2025-0702

Abstract

Abstract:

This research aims to investigate carbon sequestration microorganisms in vineyard soils and their characteristics of carbon source utilization, in order to provide a basis for enhancing soil organic matter in vineyard systems. Using the rhizosphere soil of 'Cabernet Sauvignon' grapevines as experimental material, this study employed high-throughput sequencing, screening with inorganic carbon-free media, and the Biology-ECO microplate method to analyze the community structure of carbon sequestration microorganisms and their efficiency in carbon source utilization. The dominant bacterial genera in the soils of the four 'Cabernet Sauvignon' vineyards were Bradyrhizobium, Nocardia, Mesorhizobium, Azospirillum, and Rubrivivax. Among them, the organically managed Bonabaifu Vineyard exhibited the highest richness of carbon sequestration microorganisms, with its Chao1 index being 29.70% higher than that of Hedon Vineyard and 46.10% higher than that of Gantu Vineyard. In addition, the genus Rubrivivax was identified as the key functional genus for carbon sequestration, accounting for 17.24%. The three isolated strains of carbon sequestration microorganisms all showed a preference for carbohydrate and amino acid carbon sources. This study confirmed that the carbon sequestration microorganisms in vineyards possessed relatively high species richness, and their community structures differed significantly among different vineyards. Organic and precision management practices can effectively enhance the abundance and functionality of these communities.

Key words: vineyard, rhizosphere soil, soil carbon sequestration microorganisms, carbon source utilization, Biology-ECO

YANG Siqi, XUE Tingting, ZHAO Xinru, GAO Lei, CAO Lingxiao, ZHAO Zhipeng, ZHANG Liang. Structure of Carbon Sequestration Bacterial Community and Screening of Carbon Sequestration Microorganisms in Rhizosphere Soil of Wine Grapes[J]. Chinese Agricultural Science Bulletin, 2026, 42(5): 117-127.

Figures/Tables 13

References 31

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样地	物种指数	Chao1指数	Simpson指数	Shannon指数	覆盖指数
HD	2849.80±406.09b	3174.71±376.75b	0.98±0.01a	8.46±0.88a	0.99±0.00a
HSB	3057.20±223.99b	3593.49±208.08ab	0.99±0.00a	8.93±0.31a	0.98±0.00a
GT	2321.37±685.75b	2818.38±775.24b	0.98±0.01a	7.80±0.83a	0.99±0.00a
BN	3318.87±284.19a	4117.63±329.36a	0.97±0.02a	7.76±0.51a	0.98±0.00b

样地	物种指数	Chao1指数	Simpson指数	Shannon指数	覆盖指数
HD	2849.80±406.09b	3174.71±376.75b	0.98±0.01a	8.46±0.88a	0.99±0.00a
HSB	3057.20±223.99b	3593.49±208.08ab	0.99±0.00a	8.93±0.31a	0.98±0.00a
GT	2321.37±685.75b	2818.38±775.24b	0.98±0.01a	7.80±0.83a	0.99±0.00a
BN	3318.87±284.19a	4117.63±329.36a	0.97±0.02a	7.76±0.51a	0.98±0.00b

碳源	HD	GT	HSB	CK
β-甲基-D-葡萄糖苷	0.0201±0.0054abc	0.0090±0.0010a	0.0226±0.0002bc	0.0556±0.0254a
D-半乳糖酸-γ-内酯	0.0015±0.0015e	0.0093±0.0017a	0.0000±0.0000g	0.0056±0.0050b
D-戊醛糖	0.0123±0.0085cde	0.0121±0.0016a	0.0235±0.0013b	0.0154±0.0092b
D-半乳糖醛酸	0.0156±0.0045bcd	0.0106±0.0007a	0.0265±0.0009a	0.0175±0.0100b
I-赤藓糖醇	0.0109±0.0025cde	0.0043±0.0014b	0.0000±0.0000g	0.0073±0.0049b
D-甘露醇	0.0249±0.0037ab	0.0109±0.0004a	0.0213±0.0018cd	0.0191±0.0098b
N-乙酰-D葡萄糖氨	0.0241±0.0051ab	0.0105±0.0037a	0.0190±0.0007e	0.0194±0.0097b
D-葡糖胺酸	0.0056±0.005de	0.0109±0.0026a	0.0001±0.0001g	0.0074±0.0048b
α-D-乳糖	0.0276±0.0072a	0.0124±0.0015a	0.0211±0.0005cd	0.0222±0.0124b
D,L-α-磷酸甘油	0.0125±0.0122cde	0.0094±0.0029a	0.0197±0.0020de	0.0144±0.0104b
D-纤维二糖	0.0102±0.0070cde	0.0094±0.0037a	0.0000±0.0000g	0.0086±0.0053b
葡萄糖-1-磷酸	0.0102±0.0041cde	0.0036±0.0009b	0.0085±0.0009f	0.0091±0.0051b

碳源	HD	GT	HSB	CK
β-甲基-D-葡萄糖苷	0.0201±0.0054abc	0.0090±0.0010a	0.0226±0.0002bc	0.0556±0.0254a
D-半乳糖酸-γ-内酯	0.0015±0.0015e	0.0093±0.0017a	0.0000±0.0000g	0.0056±0.0050b
D-戊醛糖	0.0123±0.0085cde	0.0121±0.0016a	0.0235±0.0013b	0.0154±0.0092b
D-半乳糖醛酸	0.0156±0.0045bcd	0.0106±0.0007a	0.0265±0.0009a	0.0175±0.0100b
I-赤藓糖醇	0.0109±0.0025cde	0.0043±0.0014b	0.0000±0.0000g	0.0073±0.0049b
D-甘露醇	0.0249±0.0037ab	0.0109±0.0004a	0.0213±0.0018cd	0.0191±0.0098b
N-乙酰-D葡萄糖氨	0.0241±0.0051ab	0.0105±0.0037a	0.0190±0.0007e	0.0194±0.0097b
D-葡糖胺酸	0.0056±0.005de	0.0109±0.0026a	0.0001±0.0001g	0.0074±0.0048b
α-D-乳糖	0.0276±0.0072a	0.0124±0.0015a	0.0211±0.0005cd	0.0222±0.0124b
D,L-α-磷酸甘油	0.0125±0.0122cde	0.0094±0.0029a	0.0197±0.0020de	0.0144±0.0104b
D-纤维二糖	0.0102±0.0070cde	0.0094±0.0037a	0.0000±0.0000g	0.0086±0.0053b
葡萄糖-1-磷酸	0.0102±0.0041cde	0.0036±0.0009b	0.0085±0.0009f	0.0091±0.0051b

碳源	HD	GT	HSB	CK
L-精氨酸	0.0017±0.0006a	0.0123±0.0027a	0.0038±0.0015b	0.0078±0.0065a
L-天门冬酰胺	0.0158±0.0070a	0.0143±0.0023a	0.0220±0.0009a	0.0159±0.0075a
L-苯丙氨酸	0.0093±0.0147a	0.0110±0.0042a	0.0000±0.0000c	0.0121±0.0126a
L-丝氨酸	0.0095±0.0048a	0.0107±0.0003a	0.0226±0.002a	0.0145±0.0097a
L-苏氨酸	0.0067±0.0048a	0.0043±0.0034b	0.0000±0.0000c	0.0058±0.0045a
甘氨酰-L-谷氨酸	0.0083±0.0042a	0.0128±0.0019a	0.0203±0.0023a	0.0140±0.0089a