Abstract: β-D-Glucosidase activity of Fusarium oxysporum isolated from different hosts and different part of cucumber and white-flowered gourd were tested. β-D-Glucosidase activity of Fusarium oxysporum isolated from different part of white-flowered gourd were 21.8233 U, 22.1663 U, 21.4573 U and 17.587 U which were almost the same. But that from different part of cucumber were different which were 23.907 U, 22.0983 U, 35.736 U and 34.5323 U. The range of β-D-Glucosidase activity of Fusarium oxysporum isolated from different hosts were 10.21830 U~39.82230 U and the sequence was watermelon＞cucumber＞white-flowered gourd＞melon＞cowpea＞capsicum.