Construction of Over-expression Vector NP24 Gene in Tomato

doi:10.11924/j.issn.1000-6850.casb15110094

Abstract

Abstract: According to the sequence of NP24 gene (GenBank registration number 543979) in NCBI, we designed primers and amplified cDNA encoding area. Then the cDNA was cloned to intermediate carrier pMD18-T. We built the over-expression vector PBI121-NP24 by inserting the gene NP24 into plant expression vector PBI121. The over-expression vector PBI121-NP24 was transferred into agrobacterium EHA105 by the CaCl₂ freeze-thaw method. Enzyme identification showed that the gene had been correctly inserted into the PBI121 carrier and the over-expression vector was built successfully. Microbial PCR identification proved that the over-expression vector had been transferred into agrobacterium.

CLC Number:

S641.2

Zhao Jingyi and Zhang Xichun. Construction of Over-expression Vector NP24 Gene in Tomato[J]. Chinese Agricultural Science Bulletin, 2016, 32(16): 138-142.

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