The Interaction Between SFB and S-RNase Protein Controlling Self-incompatibility in Xinjiang Wild Almond (Prunus tenella Batsch.)

doi:10.11924/j.issn.1000-6850.casb16120056

Abstract

Abstract: The yeast two-hybrid system was used to study the interaction between pollen SFB and column SRNase protein of self-incompatibility in Xinjiang wild almond. Taking anther from Xinjiang wild almond as material, according to the experimental requirements and structure characteristics of gene, the cloned genes SFB andS-RNase were truncated and then used to construct the yeast two hybrid recombinant expression vectors. The results showed that the yeast two hybrid recombinant expression vectors were constructed successfully. There was no interaction between PtSFB17 protein and PtS16-RNase protein or PtS17-RNase protein. Based on the above, it was presumed that PtS16-RNase protein and PtS17-RNase protein were not in the identification spectrum of PtSFB17 protein. Collaborative non-self recognition system was also in Xinjiang wild almond. There was no interaction between PtSFB17 protein and PtS16-RNase protein or PtS17-RNase protein of self incompatibility in Xinjiang wild almond.

CLC Number:

S662.9

References

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