Abstract: The study aims to analyze the climatic suitability of spring sowing zucchini in Guizhou, and provide climatic references for planting promotion of zucchini. We selected the meteorological factors of zucchini during key growth period as indexes; used the meteorological data from 1981 to 2010 and spring drought days data from 1961 to 2015 of 83 stations in Guizhou, based on ANUSPIN and ArcGIS, to apply the thin plate spline function and inverse distance weighting methods to the interpolation of each factors, and then, classified and superposed each factors according to their suitable classes, and got the layer of comprehensive climatic suitability regionalization for zucchini. We got the distribution layers of temperature, precipitation, sunshine duration and other climatic factors of zucchini during key growth period, and the most suitable, sub-suitable, and suitable region for spring sowing zucchini in Guizhou. The most suitable region distributed in the east and southeast Guizhou, which had the most suitable meteorological condition and abundant precipitation during the fruiting period, and low risk of spring drought, all could be beneficial to high yield. The sub-suitable region distributed in the north and southwest Guizhou, which had different kinds of suitable meteorological conditions and some disadvantages as well, it was necessary to strengthen field management for high yield. The suitable region distributed in the northwest Guizhou, which had relatively inferior meteorological condition and high risk of spring drought, and high yield could be obtained only through facility cultivation and enhancing the control of temperature and humidity to make full use of sunshine resources.