Super Low Temperature Technology: Research Progress and Its Application Prospect in Sugarcane Detoxication

doi:10.11924/j.issn.1000-6850.casb18100101

Abstract

Abstract:

Super-low temperature treatment is a new type of detoxification technology based on plant tissue culture and cryopreservation. To study its application prospect in detoxification of sugarcane, the authors analyzed the methods and detoxification efficiency of the treatment in common crops and the existing detoxification technology of sugarcane, summarized the principles, methods and detoxification effect of the treatment. This study could provide new ideas for sugarcane detoxification.

Key words: super-low temperature treatment, detoxication, sugarcane

CLC Number:

Wu Yuanli, Zhang Shuzhen, Tan Xianjiao, Yang Benpeng. Super Low Temperature Technology: Research Progress and Its Application Prospect in Sugarcane Detoxication[J]. Chinese Agricultural Science Bulletin, 2020, 36(6): 106-111.

Figures/Tables 5

References 41

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作物	预培养	脱水	成活率/%	参考文献
淮山药	5%蔗糖+3%甘露糖4℃,2 d	未脱水	0	[12]
淮山药	5%蔗糖+3%甘露糖4℃,2 d	60% PVS1,60 min+PVS1,100 min	77.1	[12]
甘薯	包埋,26℃,90 r/min	未脱水	0	[13]
甘薯	包埋,26℃,90 r/min	PVS,90 min	88.2	[13]
马铃薯	未经预培养	未脱水	62.5	[14]
		未脱水	90.0
		2M甘油+0.4-1M蔗糖	100.0
柑橘	MT+40 g/L蔗糖+ 0.7 mol/L甘油3 d	未脱水	0	[15]
柑橘	MT+40 g/L蔗糖+ 0.7 mol/L甘油3 d	60% PVS2,30 min+PVS2,60 min	78.6	[15]

作物	预培养	脱水	成活率/%	参考文献
淮山药	5%蔗糖+3%甘露糖4℃,2 d	未脱水	0	[12]
淮山药	5%蔗糖+3%甘露糖4℃,2 d	60% PVS1,60 min+PVS1,100 min	77.1	[12]
甘薯	包埋,26℃,90 r/min	未脱水	0	[13]
甘薯	包埋,26℃,90 r/min	PVS,90 min	88.2	[13]
马铃薯	未经预培养	未脱水	62.5	[14]
		未脱水	90.0
		2M甘油+0.4-1M蔗糖	100.0
柑橘	MT+40 g/L蔗糖+ 0.7 mol/L甘油3 d	未脱水	0	[15]
柑橘	MT+40 g/L蔗糖+ 0.7 mol/L甘油3 d	60% PVS2,30 min+PVS2,60 min	78.6	[15]

方法	前处理过程							超低温处理	解冻及培养
玻璃化法^[23]	预培养		装载	玻璃化脱水				快速冷冻	水浴解冻	卸载	恢复培养
小滴玻璃化法^[18]	预培养		装载	玻璃化脱水	形成小滴			快速冷冻	水浴解冻	卸载	恢复培养
包埋法^[3]	预培养	包埋				渗透培养	干燥	快速冷冻	水浴解冻		恢复培养
包埋玻璃化法^[4,5]	预培养	包埋	装载	玻璃化脱水				快速冷冻	水浴解冻	卸载	恢复培养

方法	前处理过程							超低温处理	解冻及培养
玻璃化法^[23]	预培养		装载	玻璃化脱水				快速冷冻	水浴解冻	卸载	恢复培养
小滴玻璃化法^[18]	预培养		装载	玻璃化脱水	形成小滴			快速冷冻	水浴解冻	卸载	恢复培养
包埋法^[3]	预培养	包埋				渗透培养	干燥	快速冷冻	水浴解冻		恢复培养
包埋玻璃化法^[4,5]	预培养	包埋	装载	玻璃化脱水				快速冷冻	水浴解冻	卸载	恢复培养

病毒名称	超低温脱毒率/%	茎尖培养脱毒率/%	其他	参考文献
马铃薯卷叶病毒(Potato leaf roll virus,PLVR)	83.0~86.0	56.0		[3]
马铃薯Y病毒(Potato virus Y,PVY)	91.0~95.0	62.0		[3]
甘薯小叶植原体(Sweet potato little leaf phytoplasma,SPLL)	100.0	0		[24]
甘薯褪绿矮化病毒(Sweet potato chlorotic stunt virus,SPCSV)	100.0	7.0		[5]
甘薯羽状斑驳病毒(Sweet potato feathery mottle virus,SPFMV)复合感染	100.0	7.0		[5]
柑桔黄龙病(Candidatus liberobacter asiaticus,HLB)	90.9~94.3	25.3		[25]
草莓轻型黄边病毒(Strawberry mild yellow edge virus,SMYEV)	95.0	0		[26]
树莓丛状矮化病毒(Raspberry bushy dwarf virus,RBDV)	33.0~35.0	0		[7]
香蕉束顶病毒(Banana bunchy top virus,BBTV)	60.6	26.7		[27]
马铃薯X病毒(Potato virus X,PVX)	59.1	36.0		[28]
洋葱黄叶矮化病毒(Onion Yellow Dwarf Virus,OYDV)	36.0			[29]
韭菜黄叶病毒(Leek Yellow Strip Virus,LYSV)	40.0
Garlic Common Latent Virus (GCLV)	32.0
Apple stem pitting virus (ASPV)	80~85 (M9) 42.5~75.2 (M26)	100.0 (M9) 100.0 (M26)		[30]
Apple stem grooving virus (ASGV)	0	0		[30]
Potato virus M	0		100.0	[31]
Potato virus S	0		100.0	[31]