New Brown Rust Resistance Gene of Sugarcane: Construction of Resistant and Susceptible Gene Pools and SSR Polymorphic Primers Screening

doi:10.11924/j.issn.1000-6850.casb2020-0125

Abstract

Abstract:

Brown rust caused by Puccinia melanocephala H. Sydow & P. Sydow is one of the main diseases that endanger sugarcane production in China. In order to identify and explore the new brown rust resistance gene, prevent the outbreaks of the disease and ensure the safe production of sugarcane, the authentic F₁ hybrid population of cross combination ‘Yuetang 03-393’× ‘ROC 24’ was used to construct resistant/susceptible gene pools, and then 449 pairs of primers were synthesized to screen SSR (simple-sequence repeat) markers that linked to resistance and susceptibility in the parental lines and the resistant/susceptible genetic pools in this study. The results showed that 25 pairs of primers were polymorphic between the parents, and among them four pairs of primers (SMC236CG, SCESSR0928, SCESSR0636, and SCESSR2551) were polymorphic between the parental lines and the resistant/susceptible genetic pools. It preliminarily indicated that the loci of these four SSR markers on chromosome might have linkage relationship with a new brown rust resistance gene. The results of this study establish a good foundation for the mapping of new brown rust resistance genes in the future, and for controlling sugarcane brown rust and breeding disease resistance varieties in China.

Key words: sugarcane, brown rust, resistant gene, resistant and susceptible gene pools, SSR marker

CLC Number:

S432.44

Wang Xiaoyan, Shan Hongli, Zhang Rongyue, Cang Xiaoyan, Wang Changmi, Li Wenfeng, Yin Jiong, Luo Zhiming, Huang Yingkun. New Brown Rust Resistance Gene of Sugarcane: Construction of Resistant and Susceptible Gene Pools and SSR Polymorphic Primers Screening[J]. Chinese Agricultural Science Bulletin, 2021, 37(6): 97-103.

Figures/Tables 3

References 35

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编号	引物		序列
72	SMC25DUQ		F: GCTTCCTAATCCATTGTTATTCTT R: GCCACTCCATCTGCTAGTGTTC
112	SC110M4-11a		F: CCACTGAGTTCCAAATCATGTCC; R: CGAGGATGTCTCCGAGGATG
155	SMC119CG		F: TTCATCTCTAGCCTACCCCAA R: AGCAGCCATTTACCCAGGA
196	SMC1760LA		F: CGCCAGGGAGATCAGCCA; R: CGTCCATTTTCAGATTGGGCTC
214	SMC2042FL		F: GGGTTCGATTCCCAGTCTG R: TTCTCGGAACGCATCTACC
215	SMC204CG		F: GCATTGGCGTCATCACTG R: TCCGTAACTGACCATTAAGAGC
230	SMC236CG		F: ACCGACCTCAAAGAAGACGGAT R: CAGTATGAGTGAAGCACGGGAAC
235	SMC24DUQ		F: CGCAACGACATATACACTTCGG R: CGACATCACGGAGCAATCAGT
237	SMC255CG		F: GATCGAGTAGGCAACAAAGCT R: CCAAAGTCACAACAGCCTCA
244	SMC286CS		F: TCAAATGGGACCTTATTGGAG R: TCCCTCGATCTCCGTTGTT
310	SMCI751CL		F: GCCATGCCCATGCTAAAGAT R: ACGTTGGTCCCGGAACCG
363	SCESSR0004		F: GTTTGGTTCCCGGAGGAGAG R: ACAATGGACACCTGAACGGC
364	SCESSR0390		F: ACAATGGACACCTGAACGGC R: GAGGAGAGAACCACAGCCGA
365	SCESSR0811		F: ACAATGGACACCTGAACGGC R: TCCCGGAGGAGATAACCACA
366	SCESSR1311		F: ACAATGGACACCTGAACGGC R: GTTTGGTTCCCGGAGGAGAG
367	SCESSR0939		F: CATTGCCCTCTCAAGCAACC R: TTTGCATCAGTCAGCCTGGA
376	MOLSSR2883		F: TGAGGTGTTGCCGTTTAATAGAGG R: ACGCGACATTCTTGAAGCTCAG
390	MOLSSR1707		F: GTGATCCCTTCCCAACGCTAC R: CACATCTCTCCTCGTCCAAAGACT
411		SCESSR0387		F: GACCAACACGGTGAACACGA R: CAGGCACGTCACCTCCTTCT
428		SCESSR0153		F: CCCGTTCGTCTCCAGCAG R: GTCGACGTGTGGGACTTCCT
435		SCESSR0928		F: CCACCGCAGCCGATATGA R: TTGAGAGGCTGACCCTGGAA
438		SCESSR2032		F: GACCTCGAGTGGTGGTTCCAT R: GTGCACCCAGTAGCACCCTC
439		MOLSSR2178		F: CGCTTTCGATTCGACTTCAACTT R: GTAGGGAGGGAAGGACCTGATG
443		SCESSR0636		F: TCCAACTTCATCCAAGTTTCCAA R: GCACCCACGAAGAGGATGAC
448		SCESSR2551		F: TGGACTAGGCCACCAGGAAA R: GTTTCGATGTGCTGCCTCCT

编号	引物		序列
72	SMC25DUQ		F: GCTTCCTAATCCATTGTTATTCTT R: GCCACTCCATCTGCTAGTGTTC
112	SC110M4-11a		F: CCACTGAGTTCCAAATCATGTCC; R: CGAGGATGTCTCCGAGGATG
155	SMC119CG		F: TTCATCTCTAGCCTACCCCAA R: AGCAGCCATTTACCCAGGA
196	SMC1760LA		F: CGCCAGGGAGATCAGCCA; R: CGTCCATTTTCAGATTGGGCTC
214	SMC2042FL		F: GGGTTCGATTCCCAGTCTG R: TTCTCGGAACGCATCTACC
215	SMC204CG		F: GCATTGGCGTCATCACTG R: TCCGTAACTGACCATTAAGAGC
230	SMC236CG		F: ACCGACCTCAAAGAAGACGGAT R: CAGTATGAGTGAAGCACGGGAAC
235	SMC24DUQ		F: CGCAACGACATATACACTTCGG R: CGACATCACGGAGCAATCAGT
237	SMC255CG		F: GATCGAGTAGGCAACAAAGCT R: CCAAAGTCACAACAGCCTCA
244	SMC286CS		F: TCAAATGGGACCTTATTGGAG R: TCCCTCGATCTCCGTTGTT
310	SMCI751CL		F: GCCATGCCCATGCTAAAGAT R: ACGTTGGTCCCGGAACCG
363	SCESSR0004		F: GTTTGGTTCCCGGAGGAGAG R: ACAATGGACACCTGAACGGC
364	SCESSR0390		F: ACAATGGACACCTGAACGGC R: GAGGAGAGAACCACAGCCGA
365	SCESSR0811		F: ACAATGGACACCTGAACGGC R: TCCCGGAGGAGATAACCACA
366	SCESSR1311		F: ACAATGGACACCTGAACGGC R: GTTTGGTTCCCGGAGGAGAG
367	SCESSR0939		F: CATTGCCCTCTCAAGCAACC R: TTTGCATCAGTCAGCCTGGA
376	MOLSSR2883		F: TGAGGTGTTGCCGTTTAATAGAGG R: ACGCGACATTCTTGAAGCTCAG
390	MOLSSR1707		F: GTGATCCCTTCCCAACGCTAC R: CACATCTCTCCTCGTCCAAAGACT
411		SCESSR0387		F: GACCAACACGGTGAACACGA R: CAGGCACGTCACCTCCTTCT
428		SCESSR0153		F: CCCGTTCGTCTCCAGCAG R: GTCGACGTGTGGGACTTCCT
435		SCESSR0928		F: CCACCGCAGCCGATATGA R: TTGAGAGGCTGACCCTGGAA
438		SCESSR2032		F: GACCTCGAGTGGTGGTTCCAT R: GTGCACCCAGTAGCACCCTC
439		MOLSSR2178		F: CGCTTTCGATTCGACTTCAACTT R: GTAGGGAGGGAAGGACCTGATG
443		SCESSR0636		F: TCCAACTTCATCCAAGTTTCCAA R: GCACCCACGAAGAGGATGAC
448		SCESSR2551		F: TGGACTAGGCCACCAGGAAA R: GTTTCGATGTGCTGCCTCCT