Abstract: By designing a pair of specific primers complementary to napA sequence, the seed - specific expression promoter of napin gene was cloned from Brassica napus Zhongyou 821 genomic DNA via polymerase chain reaction. The sequence analysis indicated that the cloned fragment contained 1148 nucleotides and possessed highly conservative sequences present in other napin promoters. The expected expression vector carrying Δ6-fatty acid desaturase gene which was isolated from Rhizopus stolonifer under the control of Napin promoter was constructed, thus making possible the subsequent production of transgenic rapeseed with highγ-linolenic acid content.