Chinese Agricultural Science Bulletin ›› 2020, Vol. 36 ›› Issue (9): 82-93.doi: 10.11924/j.issn.1000-6850.casb18120040
Special Issue: 水稻
• Research article • Previous Articles Next Articles
Yu Wang(), Xue Xu, Ruiying Qin, Xuzhong Lu, Li Li, Jianbo Yang, Jinlong Ni, Hui Ma, Xiufeng Wang
Received:
2018-12-11
Revised:
2019-01-03
Online:
2020-03-25
Published:
2020-03-29
CLC Number:
Yu Wang, Xue Xu, Ruiying Qin, Xuzhong Lu, Li Li, Jianbo Yang, Jinlong Ni, Hui Ma, Xiufeng Wang. A Method for Detecting SNP Markers Based on PCR in Rice[J]. Chinese Agricultural Science Bulletin, 2020, 36(9): 82-93.
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.casb18120040
品种类型 | 品种名称 |
---|---|
常规种/亲本 | 绍糯9714、天香58、武运粳14、高科1号、天协13、凡7、泸香658、晚粳22、R18、德香4103、宁粳3号、C418、浙农34、早籼788、HP121、中嘉早17、川农2号、皖恢9号、嘉兴8号、糯稻N-2、7NP285、镇稻15、早籼18、XH022、淮稻12、838、M1148、皖稻68、恢52、粤B、黄糯2号、密阳46、R18选、皖垦糯1号、9311、国丰一号、RH003、2428、皖稻197、ZH128、贵9B、嘉育253、红98、SR54、新强8号、R6547、中9B、五山丝苗、R118、NLjR09、粳糯4921、安香2号、II-32B、竹舟5号、川香6号、沪恢6号、早籼65、明恢100、cp120、绿稻24、明恢77、恢527、金奉19、黥香恢788、898、皖稻113、测64、盐559、安选6号、创丰占、零57、早籼15、奉化占、明63、皖稻141、丰美占、IR50、丰优58、广香籼、杨6、皖稻173、黄华占、特青、宁粳2号、皖籼316、成恢448、皖稻84、早籼14、镇江084、绿旱1号、皖稻89、培矮64、天勤1号、中籼898、Li7954、泰香4号、中籼352、万恢88、皖稻191、盐稻8号、R602、皖稻90、泰国籼、Liu3149、丰优188、中香6号、明恢1273、中早33、3263、m125、安育早1号、镇籼96、R-725、早籼2430、C4115、南京16、中籼2503、NIJR05、中香1号、粤航1号、R1846、N456、R-11、海南常规稻、肖37878、KL90、Liu矮、创优2号、IR24、CO39、明86、丰华占、明恢86 、五色黑米、五色红米、红香米、红米、红米早籼、镇籼122、4M92、8019、四丰43、青元矮、春江06、8077s、HL514、IR26、南粳15、紫稻、ZH199、GP15、IR8、黑米、IR64、B5、五常香 |
两系杂交稻 | 两优6326、广两优1128、丰两优香一号、广两优香66、II优98、徽两优6号、Y两优1号、科两优889、两优688、Y两优9918、徽两优5号、扬两优6号、深两优865、隆两优6号、丰两优一号、新华两优9号、丰两优9号、广两优76、华两优1206、II优1256、徽两优996、C两优608、深两优862、新两优香4、两优363、两优6326、淮两优3号、丰两优晚三、C两优华占、两优036、两优100、两优华6、广两优100、丰两优三号、新两优98、安两优821、常两优2号、丰两优80 |
三系杂交稻 | 中浙优8号、宜优673、冈优1577、新隆优1、甬优10号、天优998、新优188、丰晚籼优1号、春优84、五优308、金优88、辐优138、浙优18、嘉禾优555、中优1671、天优81、钱优0508、欣荣优华占、协优63、开优8号、扬籼优418、天优华占、80优121、中2优1286、甬优538、K优52、Ⅲ优98、新隆粳3号、科优8377、丰优126、协优9019、Ⅱ优8号、川香优2号、丰源优272、协优58、辐优155、甬优1540、荃香优512、丰优293、丰优512、T优15、广籼优4号、Ⅱ优107、辐优136、丰优9号、科优16、庐优855、浙糯优1号、农华优808、怀优4号 |
品种类型 | 品种名称 |
---|---|
常规种/亲本 | 绍糯9714、天香58、武运粳14、高科1号、天协13、凡7、泸香658、晚粳22、R18、德香4103、宁粳3号、C418、浙农34、早籼788、HP121、中嘉早17、川农2号、皖恢9号、嘉兴8号、糯稻N-2、7NP285、镇稻15、早籼18、XH022、淮稻12、838、M1148、皖稻68、恢52、粤B、黄糯2号、密阳46、R18选、皖垦糯1号、9311、国丰一号、RH003、2428、皖稻197、ZH128、贵9B、嘉育253、红98、SR54、新强8号、R6547、中9B、五山丝苗、R118、NLjR09、粳糯4921、安香2号、II-32B、竹舟5号、川香6号、沪恢6号、早籼65、明恢100、cp120、绿稻24、明恢77、恢527、金奉19、黥香恢788、898、皖稻113、测64、盐559、安选6号、创丰占、零57、早籼15、奉化占、明63、皖稻141、丰美占、IR50、丰优58、广香籼、杨6、皖稻173、黄华占、特青、宁粳2号、皖籼316、成恢448、皖稻84、早籼14、镇江084、绿旱1号、皖稻89、培矮64、天勤1号、中籼898、Li7954、泰香4号、中籼352、万恢88、皖稻191、盐稻8号、R602、皖稻90、泰国籼、Liu3149、丰优188、中香6号、明恢1273、中早33、3263、m125、安育早1号、镇籼96、R-725、早籼2430、C4115、南京16、中籼2503、NIJR05、中香1号、粤航1号、R1846、N456、R-11、海南常规稻、肖37878、KL90、Liu矮、创优2号、IR24、CO39、明86、丰华占、明恢86 、五色黑米、五色红米、红香米、红米、红米早籼、镇籼122、4M92、8019、四丰43、青元矮、春江06、8077s、HL514、IR26、南粳15、紫稻、ZH199、GP15、IR8、黑米、IR64、B5、五常香 |
两系杂交稻 | 两优6326、广两优1128、丰两优香一号、广两优香66、II优98、徽两优6号、Y两优1号、科两优889、两优688、Y两优9918、徽两优5号、扬两优6号、深两优865、隆两优6号、丰两优一号、新华两优9号、丰两优9号、广两优76、华两优1206、II优1256、徽两优996、C两优608、深两优862、新两优香4、两优363、两优6326、淮两优3号、丰两优晚三、C两优华占、两优036、两优100、两优华6、广两优100、丰两优三号、新两优98、安两优821、常两优2号、丰两优80 |
三系杂交稻 | 中浙优8号、宜优673、冈优1577、新隆优1、甬优10号、天优998、新优188、丰晚籼优1号、春优84、五优308、金优88、辐优138、浙优18、嘉禾优555、中优1671、天优81、钱优0508、欣荣优华占、协优63、开优8号、扬籼优418、天优华占、80优121、中2优1286、甬优538、K优52、Ⅲ优98、新隆粳3号、科优8377、丰优126、协优9019、Ⅱ优8号、川香优2号、丰源优272、协优58、辐优155、甬优1540、荃香优512、丰优293、丰优512、T优15、广籼优4号、Ⅱ优107、辐优136、丰优9号、科优16、庐优855、浙糯优1号、农华优808、怀优4号 |
序号 | 基因名称 | 表型 | 序号 | 基因名称 | 表型 |
---|---|---|---|---|---|
1 | An-1 | 芒 | 43 | Hd17 | 开花期 |
2 | An-10 | 芒 | 44 | Hd3a | 生育期 |
3 | An-9 | 芒 | 45 | Hd6 | 促进水稻北扩抽穗 |
4 | BADH2 | 籽粒香味 | 46 | HGW | 粒重 |
5 | Bh4 | 壳红 | 47 | Htd1 | 株高、分蘖 |
6 | d3 | 株高 | 48 | HTD2 | 株高 |
7 | DEP1/qPE9 | 穗节间变短,直立穗 | 49 | LAX1和SPA | 穗分枝 |
8 | DEP2/ep2 | 直立穗 | 50 | LAX2 | 产量 |
9 | DEP3 | 抽穗期 | 51 | Laxy/LA1 | 株型松散度 |
10 | DLT | 株高 | 52 | LOG | 茎分生组织活性 |
11 | DPL2 | 杂交不亲和 | 53 | MOC1 | 分蘖 |
12 | DTH2和DTH12 | 促进水稻北扩抽穗 | 54 | NAL1 | 叶型、产量 |
13 | DTH3 | 开花期 | 55 | NiR | 组培氮利用,氯酸钾解毒等 |
14 | DTH7 | 开花期 | 56 | NR | 硝酸还原酶 |
15 | DTH8/Ghd8 | 粒重、株高、抽穗期 | 57 | OSC1 | 稃尖红 |
16 | Dwarf1 | 株高 | 58 | OSCD1 | 株高 |
17 | Dwarf10 | 枝梗 | 59 | OsEBS | 株高、叶片大小、小穗数 |
18 | Dwarf14 | 枝梗 | 60 | OsPH1 | 株高 |
19 | Dwarf27 | 枝梗 | 61 | OsPIN2 | 株型 |
20 | Dwarf3 | 株高、分蘖 | 62 | OsSOC1 | 开花时间 |
21 | Dwarf53 | 枝梗 | 63 | OsSPL14; IPA1;WFP | 株型 |
22 | DWT1 | 主茎分蘖发育一致性 | 64 | OSWOX3B | 叶壳绒毛 |
23 | EG1 | 穗型 | 65 | Phr-1 | 酚染色 |
24 | Ehd1 | 生育期 | 66 | PrOG1 | 匍匐程度 |
25 | Ehd2 | 开花期 | 67 | qCTS | 抗冷性 |
26 | Ehd3 | 开花期 | 68 | qGL3 | 粒型、产量 |
27 | Ehd4 | 开花期 | 69 | Qsd12 | 休眠性 |
28 | EP2 | 穗直立性 | 70 | qSD7 | 休眠性 |
29 | EUI1 | 穗型 | 71 | qSH1/sh4/SHAT | 落粒性 |
30 | GA20x6 | 株高 | 72 | qSW5/GW5 | 粒宽 |
31 | GE | 种子发育 | 73 | Rc | 种皮果皮红 |
32 | Ghd7 | 影响株高穗粒数生育期 | 74 | Rd | 红色素 |
33 | GIF1 | 粒型 | 75 | S5-n | 广亲和 |
34 | GIP1 | 籽粒充实度 | 76 | SD1 | 株高 |
35 | GL7/GL7NR | 粒型、垩白 | 77 | Sdr4 | 休眠性 |
36 | Gn1a | 产量 | 78 | SP1 | 穗分枝数及长度 |
37 | GS3 | 粒型 | 79 | Sui1 | 穗型 |
38 | GS5 | 粒重 | 80 | TAC/qTA9 | 分蘖角大小 |
39 | GW2 | 粒长 | 81 | TAD1 | 分蘖 |
40 | GW8 | 粒重 | 82 | TGW6 | 粒重 |
41 | Hd1 | 生育期 | 83 | wx | 蜡质 |
42 | Hd16 | 开花期 |
序号 | 基因名称 | 表型 | 序号 | 基因名称 | 表型 |
---|---|---|---|---|---|
1 | An-1 | 芒 | 43 | Hd17 | 开花期 |
2 | An-10 | 芒 | 44 | Hd3a | 生育期 |
3 | An-9 | 芒 | 45 | Hd6 | 促进水稻北扩抽穗 |
4 | BADH2 | 籽粒香味 | 46 | HGW | 粒重 |
5 | Bh4 | 壳红 | 47 | Htd1 | 株高、分蘖 |
6 | d3 | 株高 | 48 | HTD2 | 株高 |
7 | DEP1/qPE9 | 穗节间变短,直立穗 | 49 | LAX1和SPA | 穗分枝 |
8 | DEP2/ep2 | 直立穗 | 50 | LAX2 | 产量 |
9 | DEP3 | 抽穗期 | 51 | Laxy/LA1 | 株型松散度 |
10 | DLT | 株高 | 52 | LOG | 茎分生组织活性 |
11 | DPL2 | 杂交不亲和 | 53 | MOC1 | 分蘖 |
12 | DTH2和DTH12 | 促进水稻北扩抽穗 | 54 | NAL1 | 叶型、产量 |
13 | DTH3 | 开花期 | 55 | NiR | 组培氮利用,氯酸钾解毒等 |
14 | DTH7 | 开花期 | 56 | NR | 硝酸还原酶 |
15 | DTH8/Ghd8 | 粒重、株高、抽穗期 | 57 | OSC1 | 稃尖红 |
16 | Dwarf1 | 株高 | 58 | OSCD1 | 株高 |
17 | Dwarf10 | 枝梗 | 59 | OsEBS | 株高、叶片大小、小穗数 |
18 | Dwarf14 | 枝梗 | 60 | OsPH1 | 株高 |
19 | Dwarf27 | 枝梗 | 61 | OsPIN2 | 株型 |
20 | Dwarf3 | 株高、分蘖 | 62 | OsSOC1 | 开花时间 |
21 | Dwarf53 | 枝梗 | 63 | OsSPL14; IPA1;WFP | 株型 |
22 | DWT1 | 主茎分蘖发育一致性 | 64 | OSWOX3B | 叶壳绒毛 |
23 | EG1 | 穗型 | 65 | Phr-1 | 酚染色 |
24 | Ehd1 | 生育期 | 66 | PrOG1 | 匍匐程度 |
25 | Ehd2 | 开花期 | 67 | qCTS | 抗冷性 |
26 | Ehd3 | 开花期 | 68 | qGL3 | 粒型、产量 |
27 | Ehd4 | 开花期 | 69 | Qsd12 | 休眠性 |
28 | EP2 | 穗直立性 | 70 | qSD7 | 休眠性 |
29 | EUI1 | 穗型 | 71 | qSH1/sh4/SHAT | 落粒性 |
30 | GA20x6 | 株高 | 72 | qSW5/GW5 | 粒宽 |
31 | GE | 种子发育 | 73 | Rc | 种皮果皮红 |
32 | Ghd7 | 影响株高穗粒数生育期 | 74 | Rd | 红色素 |
33 | GIF1 | 粒型 | 75 | S5-n | 广亲和 |
34 | GIP1 | 籽粒充实度 | 76 | SD1 | 株高 |
35 | GL7/GL7NR | 粒型、垩白 | 77 | Sdr4 | 休眠性 |
36 | Gn1a | 产量 | 78 | SP1 | 穗分枝数及长度 |
37 | GS3 | 粒型 | 79 | Sui1 | 穗型 |
38 | GS5 | 粒重 | 80 | TAC/qTA9 | 分蘖角大小 |
39 | GW2 | 粒长 | 81 | TAD1 | 分蘖 |
40 | GW8 | 粒重 | 82 | TGW6 | 粒重 |
41 | Hd1 | 生育期 | 83 | wx | 蜡质 |
42 | Hd16 | 开花期 |
序号 | SNP位点 | 上游引物01 | 上游引物02 | 下游引物 | 下游引物TM |
---|---|---|---|---|---|
1 | sf0101046878 | 5’ctttagtaagagttggactgcGtt | 5’ctttagtaagagttggactgcatg | acaaattcttactggccttcaaga | 58.615 |
2 | sf0105530098 | 5’gtgagggggggttttattggAct | 5’gtgagggggggttttattgggcg | gccagccaagtcactcacaagc | 64.436 |
3 | sf0105567692 | 5’gccagaatttaccatcaCgt | 5’gccagaatttaccatcatgc | tgctgcttctgcctctctcaca | 63.899 |
4 | sf0121636668 | 5’catccgagagtgttgcGcc | 5’catccgagagtgttgctcg | tcccgcctttcagtgtaccagt | 63.598 |
5 | sf0121636700 | 5’ctgtggcatagaaggCtg | 5’ctgtggcatagaaggatt | cctggcactcctaccatgcct | 63.953 |
6 | sf0121636862 | 5’gaattcatgaaatctccGgt | 5’gaattcatgaaatctccagc | ctggcccatgtcctccagcc | 65.068 |
7 | sf0121639360 | 5’gcttccaacctttttctctttgtaccGgc | 5’gcttccaacctttttctctttgtaccagt | gaaccctccagtgagaagaaataca | 60.28 |
8 | sf0121639399 | 5’gaggtaaattacaatgcccGtt | 5’gaggtaaattacaatgcccata | tccgtgaaatttcagaaccctcca | 62.273 |
9 | sf0121640072 | 5’gaattcatcttttcaacaTac | 5’gaattcatcttttcaacagaa | gccttccaatggacctggcca | 65.056 |
10 | sf0122352734 | 5’gcatctctcacccctttGac | 5’gcatctctcacccctttaat | tctgtattggaacggcaatgagc | 61.482 |
11 | sf0122352787 | 5’gtgaaggggacttgtccaTga | 5’gtgaaggggacttgtccaggt | tgcaggtgacagttggtccca | 63.956 |
12 | sf0122378237 | 5’ggacgcccgccggccggggcgAcg | 5’ggacgcccgccggccggggcggca | tctacgacgagccgcctcct | 65.006 |
13 | sf0122379409 | 5’gcaattcttttctaggtAcg | 5’gcaattcttttctaggtgcc | cgggcgctctaatggcattgc | 64.883 |
14 | sf0122955834 | 5’catcatcctagtagaaaCat | 5’catcctagtagaaaaag | tgtgtgccgtagtttgcatccga | 65.056 |
15 | sf0127290109 | 5’ggtaacgtccacgtgcaAtt | 5’ggtaacgtccacgtgcactg | cgtcgatcgccaccgtgtct | 64.915 |
16 | sf0127291516 | 5’gtcgatgagagaaagatctatagctaTcc | 5’gtcgatgagagaaagatctatagctagct | ctctcgtccaatccggccgg | 65.056 |
17 | sf0133308542 | 5’gacccggcgctcgccgGcg | 5’gacccggcgctcgccgact | gccctagcgcactcgtcctc | 65.029 |
18 | sf0138261916 | 5’caactagtgcttccccaggCca | 5’caactagtgcttccccaggacg | acttgcagaggactgtggga | 60.764 |
19 | sf0140333910 | 5’atcaccatcccacagcctcGct | 5’atcaccatcccacagcctctcg | acgtgtccttgccggccatt | 65.179 |
20 | sf0140361225 | 5’gttttctttttcagcattgaCgg | 5’gttttctttttcagcattgaaga | ggtctctcctggctccagcg | 64.42 |
21 | sf0140653835 | 5’agcgttcggtgAtt | 5’agcgttcggtgctc | cggccagcactctccttggg | 65.275 |
22 | sf0141821553 | 5’agtttcaaaagacttgAcg | 5’agtttcaaaagacttgccc | gaatacgcccgtgatgatgg | 58.852 |
23 | sf0202877916 | 5’gatacctccgtaaccctagcGac | 5’gatacctccgtaaccctagctat | acaactcacagagtatgccgatggt | 64.049 |
24 | sf0202880306 | 5’ccttctcttgcacatgctccaTaa | 5’ccttctcttgcacatgctccacag | ggcaaatcatctggtggtggcg | 64.58 |
25 | sf0202981582 | 5’ctgggaaaataccaactaatGtc | 5’ctgggaaaataccaactaatttg | tccaagttcagggggaatgctcc | 64.391 |
26 | sf0202981776 | 5’caggcggttaaagctAat | 5’caggcggttaaagctgac | gcctcaacctgtcctacaacct | 61.942 |
27 | sf0203835230 | 5’cgaaatgagtgttggCga | 5’cgaaatgagtgttggtgg | actcatcgagcattaccagaccg | 62.344 |
28 | sf0205785789 | 5’cactgcatcatgtcaattagAtc | 5’cactgcatcatgtcaattagctg | agcctgcactggaggggaac | 64.316 |
29 | sf0205788240 | 5’ggacaactcgatgactaccatTat | 5’ggacaactcgatgactaccatcag | agagtcaagtcggagcatggct | 63.709 |
30 | sf0209042285 | 5’gcctcggaggccaccgcTgc | 5’gcctcggaggccaccgccga | caagattgggggggctcgct | 64.739 |
31 | sf0209997735 | 5’cctgtgactttcaggaaaGac | 5’cctgtgactttcaggaaaaat | ttgctgccacctccaccacc | 65.148 |
32 | sf0219678874 | 5’ggcctgaagggggctcgAct | 5’ggcctgaagggggctcgccg | gagcgccgaatggagtgggt | 64.984 |
33 | sf0219679543 | 5’ccaatcgaggagctggaTac | 5’ccaatcgaggagctggagaa | cccgggcttcaccaccttgt | 64.865 |
34 | sf0219679569 | 5’gaggggcaaatcggtcagcgGct | 5’gaggggcaaatcggtcagcgtca | cccgggcttcaccaccttgt | 64.865 |
35 | sf0222328254 | 5’gtctacatccgccccctgcGgt | 5’gtctacatccgccccctgcagc | gagctgaacctggtcccggc | 65.265 |
36 | sf0223984419 | 5’gacgcgccgcagccctGcc | 5’gacgcgccgcagcccttca | ggcctcgttgctccggtcat | 64.984 |
37 | sf0223984848 | 5’ggacgcgctgtcggcggTct | 5’ggacgcgctgtcggcgggcg | gcgcttctccctgtaccgca | 64.942 |
38 | sf0228860823 | 5’gtcctcccccatcgcgaTgc | 5’gtcctcccccatcgcgacga | cagtggtgcatgtgacaaca | 58.976 |
39 | sf0230090897 | 5’gttggatatagctagcacCgc | 5’gttggatatagctagcacagt | tggcagaagattcatcccatctcct | 63.428 |
40 | sf0230672636 | 5’ggagatcggggaaggcgTca | 5’ggagatcggggaaggcggct | cttccggtcgtcgtcgccat | 64.944 |
41 | sf0230864756 | 5’ggatttgctgctcagccccggatcAct | 5’ggatttgctgctcagccccggatcccc | gctgtgtggtgatgcagccg | 64.6 |
42 | sf0232835231 | 5’ggagcccttgctgttcttctcGtg | 5’ggagcccttgctgttcttctcatc | gtctggagaagccggcggg | 65.227 |
序号 | SNP位点 | 上游引物01 | 上游引物02 | 下游引物 | 下游引物TM |
---|---|---|---|---|---|
1 | sf0101046878 | 5’ctttagtaagagttggactgcGtt | 5’ctttagtaagagttggactgcatg | acaaattcttactggccttcaaga | 58.615 |
2 | sf0105530098 | 5’gtgagggggggttttattggAct | 5’gtgagggggggttttattgggcg | gccagccaagtcactcacaagc | 64.436 |
3 | sf0105567692 | 5’gccagaatttaccatcaCgt | 5’gccagaatttaccatcatgc | tgctgcttctgcctctctcaca | 63.899 |
4 | sf0121636668 | 5’catccgagagtgttgcGcc | 5’catccgagagtgttgctcg | tcccgcctttcagtgtaccagt | 63.598 |
5 | sf0121636700 | 5’ctgtggcatagaaggCtg | 5’ctgtggcatagaaggatt | cctggcactcctaccatgcct | 63.953 |
6 | sf0121636862 | 5’gaattcatgaaatctccGgt | 5’gaattcatgaaatctccagc | ctggcccatgtcctccagcc | 65.068 |
7 | sf0121639360 | 5’gcttccaacctttttctctttgtaccGgc | 5’gcttccaacctttttctctttgtaccagt | gaaccctccagtgagaagaaataca | 60.28 |
8 | sf0121639399 | 5’gaggtaaattacaatgcccGtt | 5’gaggtaaattacaatgcccata | tccgtgaaatttcagaaccctcca | 62.273 |
9 | sf0121640072 | 5’gaattcatcttttcaacaTac | 5’gaattcatcttttcaacagaa | gccttccaatggacctggcca | 65.056 |
10 | sf0122352734 | 5’gcatctctcacccctttGac | 5’gcatctctcacccctttaat | tctgtattggaacggcaatgagc | 61.482 |
11 | sf0122352787 | 5’gtgaaggggacttgtccaTga | 5’gtgaaggggacttgtccaggt | tgcaggtgacagttggtccca | 63.956 |
12 | sf0122378237 | 5’ggacgcccgccggccggggcgAcg | 5’ggacgcccgccggccggggcggca | tctacgacgagccgcctcct | 65.006 |
13 | sf0122379409 | 5’gcaattcttttctaggtAcg | 5’gcaattcttttctaggtgcc | cgggcgctctaatggcattgc | 64.883 |
14 | sf0122955834 | 5’catcatcctagtagaaaCat | 5’catcctagtagaaaaag | tgtgtgccgtagtttgcatccga | 65.056 |
15 | sf0127290109 | 5’ggtaacgtccacgtgcaAtt | 5’ggtaacgtccacgtgcactg | cgtcgatcgccaccgtgtct | 64.915 |
16 | sf0127291516 | 5’gtcgatgagagaaagatctatagctaTcc | 5’gtcgatgagagaaagatctatagctagct | ctctcgtccaatccggccgg | 65.056 |
17 | sf0133308542 | 5’gacccggcgctcgccgGcg | 5’gacccggcgctcgccgact | gccctagcgcactcgtcctc | 65.029 |
18 | sf0138261916 | 5’caactagtgcttccccaggCca | 5’caactagtgcttccccaggacg | acttgcagaggactgtggga | 60.764 |
19 | sf0140333910 | 5’atcaccatcccacagcctcGct | 5’atcaccatcccacagcctctcg | acgtgtccttgccggccatt | 65.179 |
20 | sf0140361225 | 5’gttttctttttcagcattgaCgg | 5’gttttctttttcagcattgaaga | ggtctctcctggctccagcg | 64.42 |
21 | sf0140653835 | 5’agcgttcggtgAtt | 5’agcgttcggtgctc | cggccagcactctccttggg | 65.275 |
22 | sf0141821553 | 5’agtttcaaaagacttgAcg | 5’agtttcaaaagacttgccc | gaatacgcccgtgatgatgg | 58.852 |
23 | sf0202877916 | 5’gatacctccgtaaccctagcGac | 5’gatacctccgtaaccctagctat | acaactcacagagtatgccgatggt | 64.049 |
24 | sf0202880306 | 5’ccttctcttgcacatgctccaTaa | 5’ccttctcttgcacatgctccacag | ggcaaatcatctggtggtggcg | 64.58 |
25 | sf0202981582 | 5’ctgggaaaataccaactaatGtc | 5’ctgggaaaataccaactaatttg | tccaagttcagggggaatgctcc | 64.391 |
26 | sf0202981776 | 5’caggcggttaaagctAat | 5’caggcggttaaagctgac | gcctcaacctgtcctacaacct | 61.942 |
27 | sf0203835230 | 5’cgaaatgagtgttggCga | 5’cgaaatgagtgttggtgg | actcatcgagcattaccagaccg | 62.344 |
28 | sf0205785789 | 5’cactgcatcatgtcaattagAtc | 5’cactgcatcatgtcaattagctg | agcctgcactggaggggaac | 64.316 |
29 | sf0205788240 | 5’ggacaactcgatgactaccatTat | 5’ggacaactcgatgactaccatcag | agagtcaagtcggagcatggct | 63.709 |
30 | sf0209042285 | 5’gcctcggaggccaccgcTgc | 5’gcctcggaggccaccgccga | caagattgggggggctcgct | 64.739 |
31 | sf0209997735 | 5’cctgtgactttcaggaaaGac | 5’cctgtgactttcaggaaaaat | ttgctgccacctccaccacc | 65.148 |
32 | sf0219678874 | 5’ggcctgaagggggctcgAct | 5’ggcctgaagggggctcgccg | gagcgccgaatggagtgggt | 64.984 |
33 | sf0219679543 | 5’ccaatcgaggagctggaTac | 5’ccaatcgaggagctggagaa | cccgggcttcaccaccttgt | 64.865 |
34 | sf0219679569 | 5’gaggggcaaatcggtcagcgGct | 5’gaggggcaaatcggtcagcgtca | cccgggcttcaccaccttgt | 64.865 |
35 | sf0222328254 | 5’gtctacatccgccccctgcGgt | 5’gtctacatccgccccctgcagc | gagctgaacctggtcccggc | 65.265 |
36 | sf0223984419 | 5’gacgcgccgcagccctGcc | 5’gacgcgccgcagcccttca | ggcctcgttgctccggtcat | 64.984 |
37 | sf0223984848 | 5’ggacgcgctgtcggcggTct | 5’ggacgcgctgtcggcgggcg | gcgcttctccctgtaccgca | 64.942 |
38 | sf0228860823 | 5’gtcctcccccatcgcgaTgc | 5’gtcctcccccatcgcgacga | cagtggtgcatgtgacaaca | 58.976 |
39 | sf0230090897 | 5’gttggatatagctagcacCgc | 5’gttggatatagctagcacagt | tggcagaagattcatcccatctcct | 63.428 |
40 | sf0230672636 | 5’ggagatcggggaaggcgTca | 5’ggagatcggggaaggcggct | cttccggtcgtcgtcgccat | 64.944 |
41 | sf0230864756 | 5’ggatttgctgctcagccccggatcAct | 5’ggatttgctgctcagccccggatcccc | gctgtgtggtgatgcagccg | 64.6 |
42 | sf0232835231 | 5’ggagcccttgctgttcttctcGtg | 5’ggagcccttgctgttcttctcatc | gtctggagaagccggcggg | 65.227 |
序号 | SNP位点 | 上游引物01 | 上游引物02 | 下游引物 | 下游引物TM |
---|---|---|---|---|---|
43 | sf0300717646 | 5’gattctccctgaatcttcttctgCta | 5’gattctccctgaatcttcttctgatt | tgcggagacacctctttactct | 60.818 |
44 | sf0300717760 | 5’gataaccaagatgttgCac | 5’gataaccaagatgttgaat | ccagggaggtccacagcaagc | 65.309 |
45 | sf0300718947 | 5’caccaagaactggaatccGta | 5’caccaagaactggaatccatc | tgagagtacctgtcacggtga | 60.203 |
46 | sf0301270666 | 5’ccataatctcgaagactGtg | 5’ccataatctcgaagactatc | tcagttttcaattcaccaaggatgt | 59.402 |
47 | sf0303834527 | 5’gcattctaaccaattcatggaTac | 5’gcattctaaccaattcatggacat | tgacccaaacaagcaccgtggt | 65.141 |
48 | sf0304409033 | 5’ggttcctcttgtttttgcagCcg | 5’ggttcctcttgtttttgcagact | acaggcagccagtatccatgct | 64.081 |
49 | sf0306558807 | 5’gattctcttcaatgcTgg | 5’gattctcttcaatgcgga | gggaatcgcctgcaccacct | 64.983 |
50 | sf0306560299 | 5’gcttacctctcccgcttgtagCgc | 5’gcttacctctcccgcttgtagaga | ctgttcctcgccttggcgga | 64.91 |
51 | sf0306560563 | 5’ggtcgtctcggcgtcgttggcTag | 5’ggtcgtctcggcgtcgttggcgaa | ctccgagtcgtcgtgcctcg | 64.929 |
52 | sf0308410257 | 5’gcctcccagggctacaaaTtg | 5’gcctcccagggctacaaacta | acatggtttatggtgataaggctca | 60.34 |
53 | sf0308410286 | 5’gcaagaagaagtgtggccacagcaGtc | 5’gcaagaagaagtgtggccacagcaatt | accatttgcaaacaatgaaacatgc | 60.506 |
54 | sf0312107009 | 5’actggctgcggcggcggcggcggctgcGcc | 5’actggctgcggcggcggcggcggctgcact | ccacttccccacacacgcca | 65.103 |
55 | sf0312126718 | 5’gtcgaatgctgaaatgctccaTta | 5’gtcgtcgaatgctgaaatgctccagtc | ccacaggcaggagctgggaa | 64.313 |
56 | sf0312233832 | 5’cactgactttttgttattCat | 5’cactgactttttgttattaac | tgctctgagtactcgtcagt | 57.813 |
57 | sf0328506570 | 5’ggaggctgctccgccggcTac | 5’ggaggctgctccgccggcgat | gtgcaccagccggatcccag | 65.613 |
58 | sf0332993533 | 5’gcagggctgttgatttgtAta | 5’gcagggctgttgatttgtgtg | aggtcctcctgggctttgcc | 64.343 |
59 | sf0332993564 | 5’gcaaggacctccctgaCgt | 5’gcaaggacctccctgaagg | aggtcctcctgggctttgcc | 64.343 |
60 | sf0333601241 | 5’ggacttacactgcagaCaa | 5’ggacttacactgcagaaag | actccatatttctcgaggcca | 58.601 |
61 | sf0419285136 | 5’ggccgcctcgatccacgTcc | 5’ggccgcctcgatccacggcg | agccccacttcgccgacttc | 64.913 |
62 | sf0419392901 | 5’aacaccgcgcgttctacgccgTgc | 5’aacaccgcgcgttctacgccgcgg | agcacgaaccagacgccgg | 65.393 |
63 | sf0420253905 | 5’gtgcagcgcgcggggcgccgacgcTag | 5’gtgcagcgcgcggggcgccgacgcgat | cggcggtcttctcctccagc | 64.998 |
64 | sf0427382796 | 5’gaggcgcggacgaagacgccGcg | 5’gaggcgcggacgaagacgccaca | ccacgttctaccaccccgcc | 65.239 |
65 | sf0427731565 | 5’ggagtatgaccatctgAtc | 5’ggagtatgaccatctggtg | cgacaccgtgttcgatggcc | 64.012 |
66 | sf0427731615 | 5’ggtgtcggcatcttctgtttcgtAtg | 5’ggtgtcggcatcttctgtttcgtcta | caggtttcggaggacccaaact | 62.462 |
67 | sf0427746280 | 5’gtacctacatacctgaatagCcg | 5’gtacctacatacctgaatagaca | gtggtccaactccgtccact | 61.755 |
68 | sf0427754144 | 5’gttcaggaagaagccctaTag | 5’gttcaggaagaagccctacaa | cgggagactggttgaagtggacc | 64.837 |
69 | sf0432888781 | 5’gctcggcgggctccaagagTgg | 5’gctcggcgggctccaagaggga | tcgaacgccatagggacgcg | 65.002 |
70 | sf0502569493 | 5’gaagtccattgacatgttgaTac | 5’gaagtccattgacatgttgacag | atgcttcctgtgcagcacgc | 64.291 |
71 | sf0503336347 | 5’gtccgtgcgctcgcgcaTgc | 5’gtccgtgcgctcgcgcacga | ccacggggctcaccatcgac | 65.291 |
72 | sf0503339325 | 5’gtcgtacgggcacggctcTgt | 5’gtcgtacgggcacggctccgg | ttcgcggcggtcatcttcgt | 64.9 |
73 | sf0503339516 | 5’gagcgacggcggcgacggcgacggcgaTgg | 5’gagcgacggcggcgacggcgacggcgacgt | cggtagtccacgccgtcctc | 64.672 |
74 | sf0503339551 | 5’gacgccgtcgccgtcgccgtcgccgccgtcgct ctccAtc | 5’gacgccgtcgccgtcgccgtcgccgccgtcg ctctccctg | tcggcatcctcttcgccgtg | 64.983 |
75 | sf0503439783 | 5’gatcggtaggccgagtAct | 5’gatcggtaggccgagtgcg | cggcagggtcccggttatca | 64.12 |
76 | sf0527560404 | 5’gccgagctctccggcgagtaTag | 5’gccgagctctccggcgagtacaa | ccgcgttgggggtttcgattg | 64.696 |
77 | sf0527560555 | 5’gacgccatagagatgctcTat | 5’gacgccatagagatgctcgag | aggatgcctgcgccatctcc | 64.828 |
78 | sf0601764762 | 5’caggaagaacatctgcaCgt | 5’caggaagaacatctgcaagg | acgagcaatgaaagatgcatgtga | 61.634 |
79 | sf0602235646 | 5’gtttcctctatgcctccttAcg | 5’gtttcctctatgcctccttgca | tccatgaaatttcagaaccctcca | 60.203 |
80 | sf0602941293 | 5’ccggcggcaggagggtctGcc | 5’ccggcggcaggagggtctaca | gcatgcagcagatcgatcggg | 64.094 |
81 | sf0602941294 | 5’ccggcggcaggagggtctaTcc | 5’ccggcggcaggagggtctacca | gcatgcagcagatcgatcggg | 64.094 |
82 | sf0603060123 | 5’gatattattaccacagaagtCgt | 5’gatattattaccacagaagtagg | caccaccacagccgccaaga | 65.425 |
83 | sf0603060168 | 5’cctcttggcggctgtggtggtggCgg | 5’cctcttggcggctgtggtggtggtga | tgaggctgagaaggagaacccca | 64.884 |
84 | sf0604200348 | 5’atcataagctttcaagaaaatCgc | 5’atcataagctttcaagaaaatagt | gggatgctgcttgaggtttgga | 63.117 |
85 | sf0604200504 | 5’gattgccaatgcagaaggcaCat | 5’gattgccaatgcagaaggcaaag | actgacccaagccaaccagttgt | 64.699 |
86 | sf0625092934 | 5’cataacctacccgaacggtgtcgccGtg | 5’cataacctacccgaacggtgtcgccatt | tggcccggtcaatgcaacga | 65.163 |
87 | sf0626592254 | 5’gacgcagagagacatAct | 5’gacgcagagagacatgcc | ttcacgaccacgccgacgac | 65.361 |
序号 | SNP位点 | 上游引物01 | 上游引物02 | 下游引物 | 下游引物TM |
---|---|---|---|---|---|
43 | sf0300717646 | 5’gattctccctgaatcttcttctgCta | 5’gattctccctgaatcttcttctgatt | tgcggagacacctctttactct | 60.818 |
44 | sf0300717760 | 5’gataaccaagatgttgCac | 5’gataaccaagatgttgaat | ccagggaggtccacagcaagc | 65.309 |
45 | sf0300718947 | 5’caccaagaactggaatccGta | 5’caccaagaactggaatccatc | tgagagtacctgtcacggtga | 60.203 |
46 | sf0301270666 | 5’ccataatctcgaagactGtg | 5’ccataatctcgaagactatc | tcagttttcaattcaccaaggatgt | 59.402 |
47 | sf0303834527 | 5’gcattctaaccaattcatggaTac | 5’gcattctaaccaattcatggacat | tgacccaaacaagcaccgtggt | 65.141 |
48 | sf0304409033 | 5’ggttcctcttgtttttgcagCcg | 5’ggttcctcttgtttttgcagact | acaggcagccagtatccatgct | 64.081 |
49 | sf0306558807 | 5’gattctcttcaatgcTgg | 5’gattctcttcaatgcgga | gggaatcgcctgcaccacct | 64.983 |
50 | sf0306560299 | 5’gcttacctctcccgcttgtagCgc | 5’gcttacctctcccgcttgtagaga | ctgttcctcgccttggcgga | 64.91 |
51 | sf0306560563 | 5’ggtcgtctcggcgtcgttggcTag | 5’ggtcgtctcggcgtcgttggcgaa | ctccgagtcgtcgtgcctcg | 64.929 |
52 | sf0308410257 | 5’gcctcccagggctacaaaTtg | 5’gcctcccagggctacaaacta | acatggtttatggtgataaggctca | 60.34 |
53 | sf0308410286 | 5’gcaagaagaagtgtggccacagcaGtc | 5’gcaagaagaagtgtggccacagcaatt | accatttgcaaacaatgaaacatgc | 60.506 |
54 | sf0312107009 | 5’actggctgcggcggcggcggcggctgcGcc | 5’actggctgcggcggcggcggcggctgcact | ccacttccccacacacgcca | 65.103 |
55 | sf0312126718 | 5’gtcgaatgctgaaatgctccaTta | 5’gtcgtcgaatgctgaaatgctccagtc | ccacaggcaggagctgggaa | 64.313 |
56 | sf0312233832 | 5’cactgactttttgttattCat | 5’cactgactttttgttattaac | tgctctgagtactcgtcagt | 57.813 |
57 | sf0328506570 | 5’ggaggctgctccgccggcTac | 5’ggaggctgctccgccggcgat | gtgcaccagccggatcccag | 65.613 |
58 | sf0332993533 | 5’gcagggctgttgatttgtAta | 5’gcagggctgttgatttgtgtg | aggtcctcctgggctttgcc | 64.343 |
59 | sf0332993564 | 5’gcaaggacctccctgaCgt | 5’gcaaggacctccctgaagg | aggtcctcctgggctttgcc | 64.343 |
60 | sf0333601241 | 5’ggacttacactgcagaCaa | 5’ggacttacactgcagaaag | actccatatttctcgaggcca | 58.601 |
61 | sf0419285136 | 5’ggccgcctcgatccacgTcc | 5’ggccgcctcgatccacggcg | agccccacttcgccgacttc | 64.913 |
62 | sf0419392901 | 5’aacaccgcgcgttctacgccgTgc | 5’aacaccgcgcgttctacgccgcgg | agcacgaaccagacgccgg | 65.393 |
63 | sf0420253905 | 5’gtgcagcgcgcggggcgccgacgcTag | 5’gtgcagcgcgcggggcgccgacgcgat | cggcggtcttctcctccagc | 64.998 |
64 | sf0427382796 | 5’gaggcgcggacgaagacgccGcg | 5’gaggcgcggacgaagacgccaca | ccacgttctaccaccccgcc | 65.239 |
65 | sf0427731565 | 5’ggagtatgaccatctgAtc | 5’ggagtatgaccatctggtg | cgacaccgtgttcgatggcc | 64.012 |
66 | sf0427731615 | 5’ggtgtcggcatcttctgtttcgtAtg | 5’ggtgtcggcatcttctgtttcgtcta | caggtttcggaggacccaaact | 62.462 |
67 | sf0427746280 | 5’gtacctacatacctgaatagCcg | 5’gtacctacatacctgaatagaca | gtggtccaactccgtccact | 61.755 |
68 | sf0427754144 | 5’gttcaggaagaagccctaTag | 5’gttcaggaagaagccctacaa | cgggagactggttgaagtggacc | 64.837 |
69 | sf0432888781 | 5’gctcggcgggctccaagagTgg | 5’gctcggcgggctccaagaggga | tcgaacgccatagggacgcg | 65.002 |
70 | sf0502569493 | 5’gaagtccattgacatgttgaTac | 5’gaagtccattgacatgttgacag | atgcttcctgtgcagcacgc | 64.291 |
71 | sf0503336347 | 5’gtccgtgcgctcgcgcaTgc | 5’gtccgtgcgctcgcgcacga | ccacggggctcaccatcgac | 65.291 |
72 | sf0503339325 | 5’gtcgtacgggcacggctcTgt | 5’gtcgtacgggcacggctccgg | ttcgcggcggtcatcttcgt | 64.9 |
73 | sf0503339516 | 5’gagcgacggcggcgacggcgacggcgaTgg | 5’gagcgacggcggcgacggcgacggcgacgt | cggtagtccacgccgtcctc | 64.672 |
74 | sf0503339551 | 5’gacgccgtcgccgtcgccgtcgccgccgtcgct ctccAtc | 5’gacgccgtcgccgtcgccgtcgccgccgtcg ctctccctg | tcggcatcctcttcgccgtg | 64.983 |
75 | sf0503439783 | 5’gatcggtaggccgagtAct | 5’gatcggtaggccgagtgcg | cggcagggtcccggttatca | 64.12 |
76 | sf0527560404 | 5’gccgagctctccggcgagtaTag | 5’gccgagctctccggcgagtacaa | ccgcgttgggggtttcgattg | 64.696 |
77 | sf0527560555 | 5’gacgccatagagatgctcTat | 5’gacgccatagagatgctcgag | aggatgcctgcgccatctcc | 64.828 |
78 | sf0601764762 | 5’caggaagaacatctgcaCgt | 5’caggaagaacatctgcaagg | acgagcaatgaaagatgcatgtga | 61.634 |
79 | sf0602235646 | 5’gtttcctctatgcctccttAcg | 5’gtttcctctatgcctccttgca | tccatgaaatttcagaaccctcca | 60.203 |
80 | sf0602941293 | 5’ccggcggcaggagggtctGcc | 5’ccggcggcaggagggtctaca | gcatgcagcagatcgatcggg | 64.094 |
81 | sf0602941294 | 5’ccggcggcaggagggtctaTcc | 5’ccggcggcaggagggtctacca | gcatgcagcagatcgatcggg | 64.094 |
82 | sf0603060123 | 5’gatattattaccacagaagtCgt | 5’gatattattaccacagaagtagg | caccaccacagccgccaaga | 65.425 |
83 | sf0603060168 | 5’cctcttggcggctgtggtggtggCgg | 5’cctcttggcggctgtggtggtggtga | tgaggctgagaaggagaacccca | 64.884 |
84 | sf0604200348 | 5’atcataagctttcaagaaaatCgc | 5’atcataagctttcaagaaaatagt | gggatgctgcttgaggtttgga | 63.117 |
85 | sf0604200504 | 5’gattgccaatgcagaaggcaCat | 5’gattgccaatgcagaaggcaaag | actgacccaagccaaccagttgt | 64.699 |
86 | sf0625092934 | 5’cataacctacccgaacggtgtcgccGtg | 5’cataacctacccgaacggtgtcgccatt | tggcccggtcaatgcaacga | 65.163 |
87 | sf0626592254 | 5’gacgcagagagacatAct | 5’gacgcagagagacatgcc | ttcacgaccacgccgacgac | 65.361 |
序号 | SNP位点 | 上游引物01 | 上游引物02 | 下游引物 | 下游引物TM |
---|---|---|---|---|---|
88 | sf0627479781 | 5’gaaggccatgccggcTcg | 5’gaaggccatgccggcgcc | gggctcccagctccctgc | 64.892 |
89 | sf0630817105 | 5’atgaagctgcttgagaaggcGat | 5’atgaagctgcttgagaaggctag | cgaagctgttctcctttttggcgt | 64.316 |
90 | sf0630819786 | 5’gctggcctcctgaatCag | 5’gctggcctcctgaattat | aggtatctgaacatggttgacttgc | 61.32 |
91 | sf0706061910 | 5’ccatggccggcggcgaggcgTat | 5’ccatggccggcggcgaggcgcaa | ccttggtgggggcagagctg | 65.277 |
92 | sf0709151661 | 5’gggttcaagctctcccTat | 5’gggttcaagctctccccaa | gcacgttcactgacgcagcg | 65.064 |
93 | sf0709151665 | 5’gggttcaagctctccccatcGtc | 5’gggttcaagctctccccatcatt | gcacgttcactgacgcagcg | 65.064 |
94 | sf0723795969 | 5’cgtcccggccccTga | 5’cgtcccggccccggg | tcctccacctccaccaccgt | 64.883 |
95 | sf0724665141 | 5’cgagacctcattgtcacggCgg | 5’cgagacctcattgtcacggtgc | acctgtctcaccgaagtcccca | 65.022 |
96 | sf0724665404 | 5’gctcgccaatgcaCgg | 5’gctcgccaatgcaaga | gcagccaggggtttcacttgc | 64.644 |
97 | sf0725387074 | 5’aagtgacgcacccactcttgAtg | 5’aagtgacgcacccactcttggtt | gggcgtgtgctccatgatgc | 64.424 |
98 | sf0725387089 | 5’ctcttggtggttccaagttcaCtg | 5’ctcttggtggttccaagttcaatt | gggcgtgtgctccatgatgc | 64.424 |
99 | sf0729616562 | 5’ggtggccgctgcacaccggCga | 5’ggtggccgctgcacaccggagg | ggctacagcgacccggatgg | 65.087 |
100 | sf0729616641 | 5’gactatcaaagtcttgctagttAat | 5’gactatcaaagtcttgctagttgag | aggaggaaagaagagacagacct | 60.181 |
101 | sf0729626029 | 5’gatcgactggggaCgg | 5’gatcgactggggatga | tgttttgcacctcgtaaatgccg | 62.683 |
102 | sf0729626248 | 5’gttcgctgcatgataaCag | 5’gttcgctgcatgataatat | tggtgcagcatctgagtttgact | 62.634 |
103 | sf0800273114 | 5’caatttttatgggaCgc | 5’caatttttatgggaaga | gcccatgactgcttgcatggt | 63.882 |
104 | sf0800273460 | 5’aatcacatggggccaactgcaTgg | 5’aatcacatggggccaactgcacgc | agtttgccgggtgctgtcca | 64.789 |
105 | sf0800275669 | 5’ctgcctcttgcgcggtgAtg | 5’ctgcctcttgcgcggtggta | tgtccatgctgtagccgccc | 65.012 |
106 | sf0800537306 | 5’ggaagcttaatcccGca | 5’ggaagcttaatcccact | agcacaatcctccacgggct | 64.027 |
107 | sf0805352464 | 5’gtacaacagattactGcg | 5’gtacaacagattactaca | tccgtcgcatccactttcctca | 63.941 |
108 | sf0805352722 | 5’gtggggaaaatggaCgc | 5’gtggggaaaatggatgt | acatccaccttccccactgc | 62.142 |
109 | sf0805353187 | 5’ggattcatcagaaaatgcAtc | 5’ggattcatcagaaaatgcgtt | tgcgccgcttgaagcatttgt | 64.604 |
110 | sf0805353865 | 5’gttgaagaagggactaaacatcaTag | 5’gttgaagaagggactaaacatcacaa | tcctgatccatgacaactgaca | 59.094 |
111 | sf0805354180 | 5’gatggtgtccataggaaCag | 5’gatggtgtccataggaaaaa | tgattggtgttgaaatgggagcc | 61.631 |
112 | sf0805354498 | 5’ctatcaattgttgcGat | 5’ctatcaattgttgctaa | tgtagtggatcatgtctccgaaca | 61.352 |
113 | sf0819082987 | 5’gacgaggggagcggtgTag | 5’gacgaggggagcggtggat | tccaccacgactcgtcccct | 64.886 |
114 | sf0819083557 | 5’gagcgggttgctgAcc | 5’gagcgggttgctggca | gccccctcttcgtccccaac | 64.974 |
115 | sf0819084374 | 5’gacatgagggtgttgttccGgt | 5’gacatgagggtgttgttcctga | tgcccccttcttgcaatgcct | 65.025 |
116 | sf0819084634 | 5’gtcttgcaattgaagCtc | 5’gtcttgcaattgaagata | tgggtggcaatttgagcatcctga | 64.962 |
117 | sf0819463729 | 5’aactagatgatacttgcataaCgt | 5’aactagatgatacttgcataatgc | gggcaacagttatggttgca | 59.035 |
118 | sf0819464173 | 5’gcacaggaagtggctcaTcc | 5’gcacaggaagtggctcagca | cctcaaggtgagccggaag | 60.079 |
119 | sf0826502673 | 5’ggcgagatcagcttcggTac | 5’ggcgagatcagcttcggcaa | ggctggcacactgaagcagc | 64.568 |
120 | sf0826502944 | 5’ggccagagatgagaggctgcAcc | 5’ggccagagatgagaggctgcgca | gtccagccgaccgagcacat | 64.954 |
121 | sf0916786016 | 5’ggctccatgccgctctgcgCgc | 5’ggctccatgccgctctgcgtgg | gcagcttgtggccgctgatg | 64.944 |
122 | sf1018762888 | 5’ggtttcaccccaactgaTac | 5’ggtttcaccccaactgagaa | tcgatggatgctgcttagctggt | 64.2 |
123 | sf1018763187 | 5’ggtatacacaataatgttagTgg | 5’ggtatacacaataatgttagggc | tgtactttgcaagtcaggcagt | 60.421 |
124 | sf1102731365 | 5’ggtagacatcatctgtCtg | 5’ggtagacatcatctgtttt | agctaagataggactggcgaaca | 61.192 |
序号 | SNP位点 | 上游引物01 | 上游引物02 | 下游引物 | 下游引物TM |
---|---|---|---|---|---|
88 | sf0627479781 | 5’gaaggccatgccggcTcg | 5’gaaggccatgccggcgcc | gggctcccagctccctgc | 64.892 |
89 | sf0630817105 | 5’atgaagctgcttgagaaggcGat | 5’atgaagctgcttgagaaggctag | cgaagctgttctcctttttggcgt | 64.316 |
90 | sf0630819786 | 5’gctggcctcctgaatCag | 5’gctggcctcctgaattat | aggtatctgaacatggttgacttgc | 61.32 |
91 | sf0706061910 | 5’ccatggccggcggcgaggcgTat | 5’ccatggccggcggcgaggcgcaa | ccttggtgggggcagagctg | 65.277 |
92 | sf0709151661 | 5’gggttcaagctctcccTat | 5’gggttcaagctctccccaa | gcacgttcactgacgcagcg | 65.064 |
93 | sf0709151665 | 5’gggttcaagctctccccatcGtc | 5’gggttcaagctctccccatcatt | gcacgttcactgacgcagcg | 65.064 |
94 | sf0723795969 | 5’cgtcccggccccTga | 5’cgtcccggccccggg | tcctccacctccaccaccgt | 64.883 |
95 | sf0724665141 | 5’cgagacctcattgtcacggCgg | 5’cgagacctcattgtcacggtgc | acctgtctcaccgaagtcccca | 65.022 |
96 | sf0724665404 | 5’gctcgccaatgcaCgg | 5’gctcgccaatgcaaga | gcagccaggggtttcacttgc | 64.644 |
97 | sf0725387074 | 5’aagtgacgcacccactcttgAtg | 5’aagtgacgcacccactcttggtt | gggcgtgtgctccatgatgc | 64.424 |
98 | sf0725387089 | 5’ctcttggtggttccaagttcaCtg | 5’ctcttggtggttccaagttcaatt | gggcgtgtgctccatgatgc | 64.424 |
99 | sf0729616562 | 5’ggtggccgctgcacaccggCga | 5’ggtggccgctgcacaccggagg | ggctacagcgacccggatgg | 65.087 |
100 | sf0729616641 | 5’gactatcaaagtcttgctagttAat | 5’gactatcaaagtcttgctagttgag | aggaggaaagaagagacagacct | 60.181 |
101 | sf0729626029 | 5’gatcgactggggaCgg | 5’gatcgactggggatga | tgttttgcacctcgtaaatgccg | 62.683 |
102 | sf0729626248 | 5’gttcgctgcatgataaCag | 5’gttcgctgcatgataatat | tggtgcagcatctgagtttgact | 62.634 |
103 | sf0800273114 | 5’caatttttatgggaCgc | 5’caatttttatgggaaga | gcccatgactgcttgcatggt | 63.882 |
104 | sf0800273460 | 5’aatcacatggggccaactgcaTgg | 5’aatcacatggggccaactgcacgc | agtttgccgggtgctgtcca | 64.789 |
105 | sf0800275669 | 5’ctgcctcttgcgcggtgAtg | 5’ctgcctcttgcgcggtggta | tgtccatgctgtagccgccc | 65.012 |
106 | sf0800537306 | 5’ggaagcttaatcccGca | 5’ggaagcttaatcccact | agcacaatcctccacgggct | 64.027 |
107 | sf0805352464 | 5’gtacaacagattactGcg | 5’gtacaacagattactaca | tccgtcgcatccactttcctca | 63.941 |
108 | sf0805352722 | 5’gtggggaaaatggaCgc | 5’gtggggaaaatggatgt | acatccaccttccccactgc | 62.142 |
109 | sf0805353187 | 5’ggattcatcagaaaatgcAtc | 5’ggattcatcagaaaatgcgtt | tgcgccgcttgaagcatttgt | 64.604 |
110 | sf0805353865 | 5’gttgaagaagggactaaacatcaTag | 5’gttgaagaagggactaaacatcacaa | tcctgatccatgacaactgaca | 59.094 |
111 | sf0805354180 | 5’gatggtgtccataggaaCag | 5’gatggtgtccataggaaaaa | tgattggtgttgaaatgggagcc | 61.631 |
112 | sf0805354498 | 5’ctatcaattgttgcGat | 5’ctatcaattgttgctaa | tgtagtggatcatgtctccgaaca | 61.352 |
113 | sf0819082987 | 5’gacgaggggagcggtgTag | 5’gacgaggggagcggtggat | tccaccacgactcgtcccct | 64.886 |
114 | sf0819083557 | 5’gagcgggttgctgAcc | 5’gagcgggttgctggca | gccccctcttcgtccccaac | 64.974 |
115 | sf0819084374 | 5’gacatgagggtgttgttccGgt | 5’gacatgagggtgttgttcctga | tgcccccttcttgcaatgcct | 65.025 |
116 | sf0819084634 | 5’gtcttgcaattgaagCtc | 5’gtcttgcaattgaagata | tgggtggcaatttgagcatcctga | 64.962 |
117 | sf0819463729 | 5’aactagatgatacttgcataaCgt | 5’aactagatgatacttgcataatgc | gggcaacagttatggttgca | 59.035 |
118 | sf0819464173 | 5’gcacaggaagtggctcaTcc | 5’gcacaggaagtggctcagca | cctcaaggtgagccggaag | 60.079 |
119 | sf0826502673 | 5’ggcgagatcagcttcggTac | 5’ggcgagatcagcttcggcaa | ggctggcacactgaagcagc | 64.568 |
120 | sf0826502944 | 5’ggccagagatgagaggctgcAcc | 5’ggccagagatgagaggctgcgca | gtccagccgaccgagcacat | 64.954 |
121 | sf0916786016 | 5’ggctccatgccgctctgcgCgc | 5’ggctccatgccgctctgcgtgg | gcagcttgtggccgctgatg | 64.944 |
122 | sf1018762888 | 5’ggtttcaccccaactgaTac | 5’ggtttcaccccaactgagaa | tcgatggatgctgcttagctggt | 64.2 |
123 | sf1018763187 | 5’ggtatacacaataatgttagTgg | 5’ggtatacacaataatgttagggc | tgtactttgcaagtcaggcagt | 60.421 |
124 | sf1102731365 | 5’ggtagacatcatctgtCtg | 5’ggtagacatcatctgtttt | agctaagataggactggcgaaca | 61.192 |
[1] | 吴凯 . 农作物SNP芯片技术及其在分子育种中的应用[J]. 山西农业科学, 2018,46(4):670-672. |
[2] | 李欧静, 张桂华, 兰青阔 , 等. 基于SNP标记的种子纯度高效检测分析模型的建立[J]. 湖南农业科学, 2012(19):9-11,14. |
[3] | 顾渝娟, 郭建英, 程红梅 , 等. 单核苷酸多态性的检测及应用[J]. 中国农学通报, 2007,23(4):38-41. |
[4] | 赵仁欣, 李森业, 郭瑞星 , 等. 利用SNP芯片构建中国冬油菜参试品种DNA指纹图谱[J]. 作物学报, 2018,44(7):956-965. |
[5] | 黄静, 程明星, 唐敏强 , 等. 甘蓝型油菜RIL群体株高性状的全基因组关联分析[J]. 中国油料作物学报, 2016,38(5):543-548. |
[6] | 侯莉娟, 齐晓, 齐冬梅 , 等. 用于羊草基因分型的SNP分子标记技术研究[J]. 草业学报, 2016(2):105-113. |
[7] | 赵久然, 王凤格, 易红梅 , 等. 中国玉米品种标准DNA指纹库构建研究及应用进展[J]. 作物杂志, 2015(2):1-6. |
[8] | 杨润婷, 吴波, 李翀 , 等. 两种SNP分型方法的比较及其在柚品种鉴定中的应用[J]. 园艺学报, 2013(6):1061-1070. |
[9] | 徐群 . 中国籼稻品种遗传结构与多样性的SNP分析[D]. 北京:中国农业科学院, 2016: 1-88. |
[10] | BrookesA J . The essence of SNPs[J]. Gene, 1999,2(2):177-186. |
[11] | Yu H H, Xie W B, Li J , et al. A whole-genome SNP array (RICE6K) for genomic breeding in rice[J]. Plant Biotechnology Journal, 2014,1(1):28-37. |
[12] | Tung C W, Zhao K Y, Wreght M H , et al. Development of a research platform for dissecting phenotype-genotype associations in rice (Oryza spp.)[J]. Rice, 2010,4(4):205-217. |
[13] | Mccouch S R, Zhao K Y, Wright M , et al. Development of genome-wide SNP assays for rice[J]. Breeding science, 2010,5(5):524-535. |
[14] | Lee Y G, Jeong N, Kim J H , et al. Development, validation and genetic analysis of a large soybean SNP genotyping array[J]. The Plant Journal, 2015,4(4):625-636. |
[15] | 张利莎, 董国清, 扎桑 , 等. 基于EST-SSR和SNP标记的大麦麦芽纯度检测[J]. 作物学报, 2015,41(8):1147-1154. |
[16] | 陈吉宝, 景蕊莲, 员海燕 , 等. 等位基因特异PCR技术的研究与应用[J]. 植物遗传资源学报, 2005,6(4):469-473. |
[17] | 张丹丹, 周延清, 杨珂 . 基因特异性分子标记在植物育种中的研究进展[J]. 湖北农业科学, 2018,57(11):5-9. |
[18] | 熊发前, 蒋菁, 钟瑞春 , 等. 分子标记技术的两种新分类思路及目标分子标记技术的提出[J]. 中国农学通报, 2010(10):60-64. |
[19] | 李益, 马先锋, 唐浩 , 等. 柑橘品种鉴定的SSR标记开发和指纹图谱库构建[J]. 中国农业科学, 2018,51(15):149-159. |
[20] | 刘丽华, 庞斌双, 刘阳娜 , 等. 基于SNP标记的小麦高通量身份鉴定模式[J]. 麦类作物学报, 2018,38(05):529-534. |
[21] | 徐华山, 周雷, 刘凯 , 等. 水稻抗稻瘟病基因Pi35基因内特异SNP共显性分子标记开发及应用研究[J]. 现代农业科技, 2016(21):20-21. |
[22] | 马建, 马小定, 赵志超 , 等. 水稻抗稻瘟病基因Pi35功能性分子标记的开发及其应用[J]. 作物学报, 2015(12):1779-1790. |
[1] | ZHOU Dongdong, ZHANG Jun, GE Mengjie, LIU Zhonghong, ZHU Xiaohuan, LI Chunyan. Effects of Different Nitrogen Treatments on Grain Yield, Nitrogen Utilization Efficiency and Quality of Late-sowing Wheat ‘Huaimai 36’ Following Rice [J]. Chinese Agricultural Science Bulletin, 2023, 39(1): 1-7. |
[2] | Pema Rigzin, Dhonyo Dorji, Delek Kunkyi, Dekyi Yangzom, Yeshe Dorji, Penpa Tsring. Constructing the Monitoring Model of High Temperature Damage on Rice by Combining Data from Satellites and Ground Automatic Weather Stations [J]. Chinese Agricultural Science Bulletin, 2023, 39(1): 133-141. |
[3] | LUO Xianfu, LIU Wenqiang, PAN Xiaowu, DONG Zheng, LIU Sanxiong, LIU Licheng, YANG Biaoren, SHENG Xinnian, LI Xiaoxiang. Mapping of Plant Height QTL Using NILs Derived from Residual Heterozygous Lines in Rice [J]. Chinese Agricultural Science Bulletin, 2022, 38(9): 1-5. |
[4] | ZHANG Shuangyan, REN Hao, DING Wenqing, WU Yutao. Research Progress on Material Utilization of Agricultural Waste Rice Husk [J]. Chinese Agricultural Science Bulletin, 2022, 38(9): 101-108. |
[5] | HUANG Yu, CHEN Bin, XIAO Guanli. The Physiological Response of the Local Rice Variety of ‘Acuce’ of Hani Nationality in Yunnan Against the Feeding of Nilaparvata lugens Stål [J]. Chinese Agricultural Science Bulletin, 2022, 38(9): 123-129. |
[6] | SHI Yonghai, CAO Xiangde, XU Jiabo. Effect of COVID-19 Epidemic on Alosa sapidissima Production in China and the Countermeasures [J]. Chinese Agricultural Science Bulletin, 2022, 38(9): 151-156. |
[7] | LI Xinghua, WANG Huan, ZHANG Sheng, CAI Xingxing, ZHOU Qiang, ZHOU Nan. Nitrogen Application Rate and Mode: Effects on Yield and Dry Matter Accumulation and Transport After Flowering of Late Indica Rice [J]. Chinese Agricultural Science Bulletin, 2022, 38(9): 6-13. |
[8] | YE Pei, LIU Kequn, SHEN Shuanghe, LIU Kaiwen, LIU Zhixiong, DENG Yanjun. Risk Analysis and Regionalization of Heat Damage During Heading and Flowering Stage of Mid-season Rice in Hubei Province [J]. Chinese Agricultural Science Bulletin, 2022, 38(8): 110-117. |
[9] | WANG Yifan, LAO Xiaocan, YU Liping, YE Hailong. Rice Variety ‘Yongyou 15’: The Suitability of Meteorological Conditions for Sowing by Stages [J]. Chinese Agricultural Science Bulletin, 2022, 38(7): 106-109. |
[10] | LIU Xiaohang, MA Shuqing, ZHAO Jing, QUAN Hujie, DENG Kuicai, CHAI Qingrong. Yield Response of Japonica Rice of Northeast China to Low Temperature in Different Time Periods of Booting Stage [J]. Chinese Agricultural Science Bulletin, 2022, 38(7): 91-98. |
[11] | LI Xuefeng, WANG Jian, YE Xiaoyuan, ZHANG Xiuting, WANG Lixue. Plant Aqueous Extract of Momordica charantia: Effects on Rice Seed Germination and Seedling Growth [J]. Chinese Agricultural Science Bulletin, 2022, 38(6): 1-7. |
[12] | YAN Yuntao, HE Xi, ZHANG Haiqing, HE Jiwai. Advances in Research on the Storability of Rice Seeds [J]. Chinese Agricultural Science Bulletin, 2022, 38(5): 1-8. |
[13] | ZHAI Caijiao, ZHANG Jiao, CUI Shiyou, CHEN Pengjun. Effects of Salt Stress on the Panicle Traits and Yield Components of Rice Cultivars [J]. Chinese Agricultural Science Bulletin, 2022, 38(4): 1-9. |
[14] | LIU Yuting, HUANG Shiyu, LI Liujia, ZHAO Tianzhang, LI Huiying, SU Zifeng, LONG Xiaowen. Comparative Study on Biological Indexes and Meat Nutritional Value of Cyprinus carpio Under Earth Pond Reared Mode and Rice Field Reared Mode [J]. Chinese Agricultural Science Bulletin, 2022, 38(4): 159-164. |
[15] | HU Ping, FENG Minyu, WU Fengyu, CAO Heyu. Suitable Sowing Temperature for Direct Seeding Early Rice [J]. Chinese Agricultural Science Bulletin, 2022, 38(35): 70-75. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||