Flavonoids of Toona sinensis Leaves: Separating and Purifying with D-101 Macroporous Adsorptive Resin and the Antioxidant Activity

doi:10.11924/j.issn.1000-6850.casb20190800516

Abstract

Abstract:

The flavonoids in leaves of Toona sinensis were separated and purified with D-101 macroporous resin to get the utmost out of the leaves. The adsorptive capacity and stability of D-101 macroporous resin for flavonoids from T. sinensis leaves were studied with the adsorption rate, elution rate and purity of total flavonoids as indexes. At the same time, the antioxidant activity of T. sinensis flavonoids was evaluated by scavenging 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radicals. The results showed that the optimum sampling condition of D-101 macroporous resin was: the sample size of T. sinensis extract was 5 BV, the flavonoids concentration was 0.69 mg/mL, and the sampling flow rate was 3 BV/h. The optimum elution condition was: 70% ethanol was used as eluent, the dosage of eluent was 5 BV, and the flow rate was 3 BV/h. When the resin was used once, the purity of flavonoids of T. sinensis could be increased from 6.0% to 45.14%, and the resin was stable and could be reused for 6 times before regeneration. The flavonoids isolated and purified by macroporous resin had strong antioxidant activity, which was 1.33 times as much as ascorbic acid.

Key words: flavonoids, separation and purification, D-101 macroporous resin, T. sinensis leaves, antioxidant activity

CLC Number:

TS202.3

Zou Hongfei, Shu Xiaoyan, Zhang Yueqin, Liu Yuan, Yang Wenyu, Zhao Guiying. Flavonoids of Toona sinensis Leaves: Separating and Purifying with D-101 Macroporous Adsorptive Resin and the Antioxidant Activity[J]. Chinese Agricultural Science Bulletin, 2020, 36(24): 159-164.

Figures/Tables 7

References 27

[1]	肖华山, 肖祥希, 何文广, 等. 香椿种质资源与快速繁殖研究进展(综述)[J]. 亚热带植物科学, 2014,43(4):343-346.
[2]	周琼. 嫩芽味美郁椿香[J]. 食品与健康, 2016(3):18.
[3]	孙小祥. 香椿叶的化学成分研究及黄酮类化合物的分析[D]. 镇江:江苏大学, 2016.
[4]	Chen C, Liu B, Han F, et al. Antioxidant and Cytotoxic Activity of the Ethanol Extract from Red Toon Leaves[A]. International Conference on Biomedical Engineering & Computer Science[C]. 2010.
[5]	李凤玉, 高鷃铭, 肖祥希, 等. 香椿叶醇提取物对果蝇寿命及抗氧化作用的影响[J]. 福建师范大学学报:自然科学版, 2017(4):59-64.
[6]	George V C, Dellaire G, Rupasinghe H P V. Plant flavonoids in cancer chemoprevention: role in genome stability[J]. Journal of Nutritional Biochemistry, 2017,45:1-14. doi: 10.1016/j.jnutbio.2016.11.007 URL pmid: 27951449
[7]	Nile S H, Keum Y S, Nile A S, et al. Antioxidant, anti-inflammatory, and enzyme inhibitory activity of natural plant flavonoids and their synthesized derivatives[J]. Journal of Biochemical & Molecular Toxicology, 2017,32(1). doi: 10.1002/jbt.22001 URL pmid: 28960612
[8]	项薇, 潘速跃, 武肖娜, 等. 银杏叶黄酮对急性脑梗死模型小鼠凋亡因子的影响[J]. 中国临床药理学杂志, 2017(8).
[9]	Li H, Luo K, Hou J. Inhibitory effect of Puerariae radix flavones on platelet-derived growth factor-BB-induced proliferation of vascular smooth muscle cells via PI3K and ERK pathways[J]. Experimental and therapeutic medicine, 2015,9(1):257-261. doi: 10.3892/etm.2014.2074 URL pmid: 25452812
[10]	Wu Y, Lei Z, Mao J, et al. Kinetic and thermodynamic studies of sulforaphane adsorption on macroporous resin[J]. Journal of Chromatography B Analytical Technologies in the Biomedical & Life Sciences, 2016,1028:231-236. doi: 10.1016/j.jchromb.2016.06.035 URL pmid: 27391585
[11]	耿天华. 响应面法优化大孔树脂纯化昆仑雪菊色素的工艺[J]. 中国中药杂志, 2016,41(2):220-225.
[12]	汤如莹. D-101型大孔吸附树脂分离纯化沙苑子总黄酮工艺研究[J]. 中草药, 2017,48(16):3342-3346.
[13]	Zhao Y N, Wang Z L, Dai J G, et al. Preparation and quality assessment of high-purity ginseng total saponins by ion exchange resin combined with macroporous adsorption resin separation[J]. Chinese Journal of Natural Medicines, 2014,12(5):382-392. doi: 10.1016/S1875-5364(14)60048-0 URL pmid: 24856763
[14]	王昌禄, 李贞景, 李风娟, 等. 香椿叶总黄酮提取工艺研究[J]. 四川中医, 2011(2):48-50.
[15]	刘涛, 李娟, 郭晓恒, 等. D-101大孔树脂用于中药精制工艺预处理方法优化研究[J]. 南京中医药大学学报, 2012,28(5):474-476.
[16]	陈丛瑾, 黄克瀛, 李德良, 等. NaNO₂-Al(NO₃)₃显色分光光度法测定香椿叶总黄酮的含量[J]. 食品研究与开发, 2007,28(5):104-107.
[17]	陈建中, 葛水莲, 昝立峰, 等. 响应面优化大孔吸附树脂分离纯化茼蒿总黄酮[J]. 食品与发酵工业, 2015,41(11):115-120.
[18]	于鹤云, 刘汉清, 刘嘉, 等. D-101型大孔吸附树脂纯化黄蜀葵花总黄酮的工艺研究[J]. 中成药, 2014,36(3):520-525.
[19]	陈丛瑾, 黄克瀛, 李德良, 等. 香椿叶提取物清除DPPH自由基能力的测定方法[J]. 林产化学与工业, 2006,26(3):69-72.
[20]	王晓敏, 杨慧, 张乐, 等. 香椿废弃组织中总黄酮提取工艺优化及抗氧化活性研究[J]. 食品工业科技, 2016,37(11):232-237.
[21]	李光辉, 孙思胜, 王德国, 等. 香椿老叶黄酮的响应面优化提取及其抗氧化研究[J]. 许昌学院学报, 2018,37(6):51-55.
[22]	张志清, 向建军, 周利茗, 等. 麦苗中阿魏酸、抗坏血酸(Vc)、总黄酮协同抗氧化能力分析[J]. 中国粮油学报, 2013,28(7):8.
[23]	王付荣, 杨雁芳, 杨秀伟. 通脉方中异黄酮类成分清除DPPH自由基的活性研究[J]. 中国现代中药, 2017,19(11):1545-1549.
[24]	韦献雅, 殷丽琴, 钟成, 等. DPPH法评价抗氧化活性研究进展[J]. 食品科学, 2014,35(9):317-322. doi: 10.7506/spkx1002-6630-201409062 URL
[25]	袁园, 张潇, 陈碧琼, 等. 草果总黄酮的提取及DPPH自由基清除活性研究[J]. 食品研究与开发, 2017(15):63-68.
[26]	熊利芝, 李佳焱, 王家坚, 等. D-101大孔吸附树脂对黄花蒿黄酮的吸附热力学和动力学研究[J]. 离子交换与吸附, 2014(4):334-342.
[27]	叶勇, 施敏荣. D-101大孔树脂对丹酚酸B分离工艺的优化[J]. 中国药学杂志, 2006,41(20):1557-1560.