Evaluation of Drought Tolerance of Maize Seedlings Based-on Gold Nanoparticles Synthesis Method

doi:10.11924/j.issn.1000-6850.casb20200100023

Abstract

Abstract:

To detect the antioxidant activity of maize seedlings before and after drought stress by using gold nanoparticles (GNPs) synthesis method and then evaluate the drought tolerance of maize seedlings, four maize varieties (‘Zhengdan 958’, ‘Xundan 20’, ‘Denghai 662’ and ‘Yuyu 22’) were selected and their antioxidant activity was detected by GNPs synthesis method. GNPs synthesis method was used to test the antioxidant activity, and the conditions were optimized and methodologically confirmed. GNPs were characterized by transmission electron microscope (TEM) and X-ray photoelectron spectroscopy (XPS). At the same time, the results of DPPH, FRAP and FC methods were compared. The methods including phenotypic identification, SOD activity and MDA content determination were used to detect the drought tolerance of maize seedlings. Correlation analysis and principal component analysis were performed on the detection results of the above methods. The results showed that the antioxidant activity of seedlings of the four maize varieties was from strong to weak detected by GNPs synthesis method as: ‘Zhengdan 958’> ‘Xundan 20’> ‘Denghai 662’> ‘Yuyu 22’, which was consistent with the results of traditional methods and drought resistance detection. The results of correlation analysis and principal component analysis showed that the GNPs synthesis method was well correlated with other methods and had a high expression in explaining the total variation, indicating that it is feasible to evaluate the drought resistance of maize seedlings.

Key words: gold nanoparticles, maize seedling, detection, antioxidant activity, evaluation, drought tolerance

CLC Number:

S513

Wei Dongwei, Xie Juanjuan, Zhou Yaping, Sun Wuyong, Ren Yuanyu. Evaluation of Drought Tolerance of Maize Seedlings Based-on Gold Nanoparticles Synthesis Method[J]. Chinese Agricultural Science Bulletin, 2020, 36(34): 5-14.

Figures/Tables 10

References 26

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品种	处理	自由基清除率/ %	硫酸亚铁当量/ (mmol/L)	没食子酸当量/ (mg GAE/g)	品种	处理	自由基清除率/ %	硫酸亚铁当量/ (mmol/L)	没食子酸当量/ (mg GAE/g)
郑单958	对照	38.80	0.645	3.60	登海662	对照	37.44	0.615	3.43
	胁迫	44.29	0.845	3.92		胁迫	42.05	0.787	3.44
	胁迫-对照	5.49↑	0.200↑	0.32↑		胁迫-对照	4.16↑	0.172↑	0.01↑
浚单20	对照	42.75	0.737	3.21	豫玉22	对照	36.04	0.669	3.16
	胁迫	43.50	0.802	3.40		胁迫	39.67	0.728	2.77
	胁迫-对照	0.75↑	0.065↑	0.19↑		胁迫-对照	3.64↑	0.059↑	0.39↓

品种	处理	自由基清除率/ %	硫酸亚铁当量/ (mmol/L)	没食子酸当量/ (mg GAE/g)	品种	处理	自由基清除率/ %	硫酸亚铁当量/ (mmol/L)	没食子酸当量/ (mg GAE/g)
郑单958	对照	38.80	0.645	3.60	登海662	对照	37.44	0.615	3.43
	胁迫	44.29	0.845	3.92		胁迫	42.05	0.787	3.44
	胁迫-对照	5.49↑	0.200↑	0.32↑		胁迫-对照	4.16↑	0.172↑	0.01↑
浚单20	对照	42.75	0.737	3.21	豫玉22	对照	36.04	0.669	3.16
	胁迫	43.50	0.802	3.40		胁迫	39.67	0.728	2.77
	胁迫-对照	0.75↑	0.065↑	0.19↑		胁迫-对照	3.64↑	0.059↑	0.39↓

检测方法	主成分1	主成分2	表达量
GNPs	0.902	0.385	0.647
MDA	0.926	0.010	0.605
DPPH	0.526	0.826	0.519
FRAP	-0.020	0.980	0.360
SOD	0.870	-0.333	0.358
FC	0.792	-0.609	0.210

检测方法	主成分1	主成分2	表达量
GNPs	0.902	0.385	0.647
MDA	0.926	0.010	0.605
DPPH	0.526	0.826	0.519
FRAP	-0.020	0.980	0.360
SOD	0.870	-0.333	0.358
FC	0.792	-0.609	0.210