Mutagenesis of Kidney Bean Cultivar A18-1 by EMS and Mutant Library Construction

doi:10.11924/j.issn.1000-6850.casb20191201000

Abstract

Abstract:

Kidney bean (Phaseolus vulgaris L.) as an important economic crop is widely planted. It is characterized by many varieties, obvious phenotypic differences and small genome, and has become an important material for studying the genome function of legume plants. In order to create more mutant materials for improving the varieties and enriching the mutant library, and to accumulate the materials for study genome function of legume plants in the future, dried kidney bean seeds of A18-1 were treated with 0.04 mol/L ethyl methanesulfonate (EMS), 2128 M₂ plants were conducted with field phenotype selection during the whole growth period, and 107 mutants were screened, which accounted for 5.03% of the total population. The mutants were constructed with trait variations on leaf, stem, flower, pod, plant type, fertility and maturity. Among them, the number and the type of mutants related to leaf shape and leaf color were the most, accounted for 27.0%. But the number of mutants related to stems, flowers and fertility was equal and the least, taking up 10.3% of total mutants. This experiment could provide new materials for constructing and innovating mutant libraries, and it is of great significance for studying specific relationship between genome function and phenotype as well as genetic breeding.

Key words: kidney bean, ethyl methanesulfonate (EMS), mutagenesis, mutant library, phenotypic screening

CLC Number:

S643.1

Qin Xiangkai, Feng Guojun, Liu Dajun, Liu Chang, Yang Xiaoxu, Yan Zhishan. Mutagenesis of Kidney Bean Cultivar A18-1 by EMS and Mutant Library Construction[J]. Chinese Agricultural Science Bulletin, 2020, 36(35): 14-21.

Figures/Tables 7

References 25

[1]	Cominelli E, Confalonieri M, Carlessi M, et al. Phytic acid transport in, Phaseolus vulgaris: A new, low phytic acid, mutant in the, PvMRP1, gene and study of the, PvMRPs, promoters in two different plant systems[J]. Plant Science, 2018,270:1-12. doi: 10.1016/j.plantsci.2018.02.003 URL pmid: 29576062
[2]	Mago R, Till B, Periyannan S, et al. Generation of Loss-of-Function Mutants for Wheat Rust Disease Resistance Gene Cloning[M] // Wheat Rust Diseases. Methods Mol Biol, 2017.
[3]	陈其福, 刘畅, 斯琴图雅, 等. 菜豆A18-1~(60)Co-γ射线诱变和突变体库的构建[J]. 中国蔬菜, 2019,363(05):43-49.
[4]	郭宁, 郑佳佳, 胡博, 等. 菜豆品种黄金勾伽玛射线突变体库的建立及突变表型观察[J]. 土壤与作物, 2018,7(2).
[5]	Sakowska K, Alberti G, Genesio L, et al. Leaf and canopy photosynjournal of a chlorophyll deficient soybean mutant[J]. Plant Cell and Environment, 2018,41(6).
[6]	Wang Y P, Tang S Q, Wu Z F, et al. Phenoty picanaly sisofadwar fanddeformed ower3 (ddf3) mutantinric (Oryza sativa L.) and characterization of candidate genes[J]. Journal of Integrative Agriculture, 2018,17(5):1057-1065. doi: 10.1016/S2095-3119(17)61770-2 URL
[7]	Lee J Y, Kang C S, Beom H R, et al. Characterization of a wheat mutant missing low-molecular-weight glutenin subunits encoded by the B-genome[J]. Journal of Cereal Science, 2017,73:158-164. doi: 10.1016/j.jcs.2016.12.004 URL
[8]	Vimala V, Pushpamma P. Elongation and Gravireactivity of Roots from an Agravitropic Maize Mutant: Implications of Growth Inhibitors[J]. Plant & Cell Physiology, 2017,24(3):333-336.
[9]	Bechere E, Zeng L, Auld D. Registration of Five Upland Cotton Mutant Germplasm Lines with Superior Fiber Length, Strength, and Uniformity[J]. Journal of Plant Registrations, 2018,12(1).
[10]	Galpaz N, Wang Q, Menda N, et al. Abscisic acid deficiency in the tomato mutant high-pigment 3 leading to increased plastid number and higher fruit lycopene content[J]. The Plant Journal, 2008,53(5):717-730. doi: 10.1111/j.1365-313X.2007.03362.x URL pmid: 17988221
[11]	Huan Z, Qian Z, Yan-Nan W, et al. Characterization of salt tolerance and Fusarium wilt resistance of a sweetpotato mutant[J]. Journal of Integrative Agriculture, 2017,16(9):1946-1955. doi: 10.1016/S2095-3119(16)61519-8 URL
[12]	Ma C, Wang Y, Gu D, et al. Overexpression of S-Adenosyl-l-Methionine Synthetase 2 from Sugar Beet M14 IncreasedArabidopsisTolerance to Salt and Oxidative Stress:[J]. International Journal of Molecular Sciences, 2017,18(4):847. doi: 10.3390/ijms18040847 URL
[13]	Talukdar D. Mutagenesis as functional biology tool in the improvement of legumes, mutagenesis: exploring novel genes and pathways[M]. Wageningen: Wageningen Academic Publishers: 2014(2):37-72.
[14]	冯国军, 刘大军. 菜豆的营养价值评价与分析[J]. 北方园艺, 2016(24):200-208.
[15]	李正丽, 文林宏, 李桂莲, 等. 菜豆栽培技术[J]. 农技服务, 2018(1):38-42.
[16]	Wang X, Chen E, Ge X, et al. Overexpressed BRH1, a RING finger gene, alters rosette leaf shape in Arabidopsis thaliana[J]. Science China Life Sciences, 2018,61(1):1-9. doi: 10.1007/s11427-017-9265-8 URL pmid: 29349729
[17]	Wu L, Tian Z, Zhang J. Functional dissection of auxin response factors in regulating tomato leaf shape development[J]. Frontiers in Plant Science, 2018,9:957. doi: 10.3389/fpls.2018.00957 URL pmid: 30022995
[18]	张力科, 高用明. 水稻叶色突变体及其基因定位和克隆的研究进展[J]. 作物杂志, 2009(2):12-16.
[19]	张力科, 李志彬, 刘海燕, 等. 两个新的水稻叶色突变体形态结构与遗传定位研究[J]. 中国农业科学, 2010,43(2):223-229.
[20]	谭炎宁, 孙学武, 袁定阳, 等. 水稻单叶独立转绿型黄化突变体grc2的鉴定与基因精细定位[J]. 作物学报, 2015,41(6):831-837. doi: 10.3724/SP.J.1006.2015.00831 URL
[21]	Cao H, Li H, Miao Z, et al. The Preliminary Study of Leaf-color Mutant in Dendrobium officinale[J]. Journal of Nuclear Agricultural Sciences, 2017.
[22]	Li W, Tian Z X, Yu D Q. WRKY13 acts in stem development in Arabidopsis thaliana[J]. Plant Science, 2015,236:205-213. doi: 10.1016/j.plantsci.2015.04.004 URL pmid: 26025534
[23]	王传堂, 唐月异, 徐建志, 等. 化学诱变剂EMS诱发花生荚果性状变异的研究[J]. 花生学报, 2008,37(2):5-9.
[24]	杨建胜, 孙万仓, 刘自刚, 等. 化学诱变剂EMS对白菜型冬油菜陇油7号形态及生理生化的影响[J]. 西北农业学报, 2015,24(3).
[25]	Toussaint A, Baudoin J P, Mergeai G, et al. Description of Phaseolus vulgaris L. aborting embryos from ethyl methanesulfonate (EMS) mutagenized plants[J]. Biotechnologie Agronomie Societe Et Environment, 2013,17(4):563.

突变类型	突变数量	突变率/%	突变性状描述
叶	29	1.36	细长、圆形、黄化、黄化后转绿、白化、深绿
茎	11	0.52	茎粗、茎细、茎酒红色
花及育性	11	0.52	花结构变异、花序异常
荚	19	0.89	荚多、荚变大、绿且弯、小且弯
株型	19	0.89	株高、株矮小、抗性好
熟期	18	0.85	早熟、晚熟、早衰
总计	107	5.03

突变类型	突变数量	突变率/%	突变性状描述
叶	29	1.36	细长、圆形、黄化、黄化后转绿、白化、深绿
茎	11	0.52	茎粗、茎细、茎酒红色
花及育性	11	0.52	花结构变异、花序异常
荚	19	0.89	荚多、荚变大、绿且弯、小且弯
株型	19	0.89	株高、株矮小、抗性好
熟期	18	0.85	早熟、晚熟、早衰
总计	107	5.03