Chinese Agricultural Science Bulletin ›› 2021, Vol. 37 ›› Issue (15): 106-111.doi: 10.11924/j.issn.1000-6850.casb2020-0372
Special Issue: 生物技术
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Yuan Guohui(), Tian Zhihui, Li Tao, Qian Zhenguan, Shen Guohui()
Received:
2020-08-18
Revised:
2020-11-16
Online:
2021-05-25
Published:
2021-05-18
Contact:
Shen Guohui
E-mail:yuanguohui@saas.sh.cn;ghshen65@163.com
CLC Number:
Yuan Guohui, Tian Zhihui, Li Tao, Qian Zhenguan, Shen Guohui. DNA Barcodes Study of Echinochloa[J]. Chinese Agricultural Science Bulletin, 2021, 37(15): 106-111.
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.casb2020-0372
条形码序列 | 引物名称 | 引物序列(5’-3’) | 退火温度/℃ |
---|---|---|---|
ITS | ITS4 | TCCTCCGCTTATTGATATGC | 56 |
ITS-Y5 | TAGAGGAAGGAGAAGTCGTAACAA | ||
ETS | RETS4_F | TGGCTACGCGAGCGCATGAG | 56 |
18S_R | AGACAAGCATATGACTACTGGCAGG | ||
psbA | #1-F | GTGCCTACTCGGCATTTCAC | 56 |
#1-R | GTTGATAGCCAAGGTCGCGT | ||
trnL-F | trn-F | ATTTGAACTGGTGACACGAG | 54 |
trn-C | CGAAATCGGTAGACGCTACG | ||
matK | W | TACCCTATCCTATCCAT | 55~50 touch down |
trnK-2R | AACTAGTCGGATGGAGTAG |
条形码序列 | 引物名称 | 引物序列(5’-3’) | 退火温度/℃ |
---|---|---|---|
ITS | ITS4 | TCCTCCGCTTATTGATATGC | 56 |
ITS-Y5 | TAGAGGAAGGAGAAGTCGTAACAA | ||
ETS | RETS4_F | TGGCTACGCGAGCGCATGAG | 56 |
18S_R | AGACAAGCATATGACTACTGGCAGG | ||
psbA | #1-F | GTGCCTACTCGGCATTTCAC | 56 |
#1-R | GTTGATAGCCAAGGTCGCGT | ||
trnL-F | trn-F | ATTTGAACTGGTGACACGAG | 54 |
trn-C | CGAAATCGGTAGACGCTACG | ||
matK | W | TACCCTATCCTATCCAT | 55~50 touch down |
trnK-2R | AACTAGTCGGATGGAGTAG |
条形码序列 | 序列长度/bp | G+C含量/% | 变异位点/% | 种内变异 | 种间变异 | 变异分辨率/% |
---|---|---|---|---|---|---|
ITS | 704~705 | 58.7~59.1 | 2.0 | 0.0012±0.0002 | 0.0036±0.0015 | 50.0 |
ETS | 472 | 58.9~59.3 | 1.7 | 0.0011±0.0008 | 0.0032±0.0025 | 48.8 |
psbA | 337 | 38.3~38.9 | 2.7 | 0.0015±0.0011 | 0.0060±0.0044 | 60.0 |
trnL-F | 951~956 | 35.0~35.4 | 3.0 | 0.0022±0.0009 | 0.0048±0.0026 | 37.1 |
matK | 1330~1331 | 33.7~34.1 | 3.6 | 0.0029±0.0007 | 0.0048±0.0016 | 24.7 |
条形码序列 | 序列长度/bp | G+C含量/% | 变异位点/% | 种内变异 | 种间变异 | 变异分辨率/% |
---|---|---|---|---|---|---|
ITS | 704~705 | 58.7~59.1 | 2.0 | 0.0012±0.0002 | 0.0036±0.0015 | 50.0 |
ETS | 472 | 58.9~59.3 | 1.7 | 0.0011±0.0008 | 0.0032±0.0025 | 48.8 |
psbA | 337 | 38.3~38.9 | 2.7 | 0.0015±0.0011 | 0.0060±0.0044 | 60.0 |
trnL-F | 951~956 | 35.0~35.4 | 3.0 | 0.0022±0.0009 | 0.0048±0.0026 | 37.1 |
matK | 1330~1331 | 33.7~34.1 | 3.6 | 0.0029±0.0007 | 0.0048±0.0016 | 24.7 |
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