Screening and Identification of a Cellulose Degrading Strain

doi:10.11924/j.issn.1000-6850.casb2021-0131

Abstract

Abstract:

To solve the problem of low degradation rate of corn straw, stable cellulose degrading bacteria were screened from natural fermentation compost with amino acid tail liquor as the nitrogen source, aiming to lay a foundation for the preparation of efficient straw decomposition agent. Congo red staining method was adopted for preliminary screening, the determination of cellulase activity and the loss rate of corn straw weight were used for re-screening, and morphological and molecular biological methods were used to identify the efficient degrading strain. The results showed that an efficient cellulose degrading strain, SC2, was obtained. The filter paper enzyme activity, endoglucanase activity, exoglucanase activity and β-glucosidase activity of SC2 was 17.70 U/mL, 58.97 U/mL, 16.85 U/mL and 79.26 U/mL, respectively. The degradation rate of corn straw reached 33.07%. According to the colony characteristics, sporulation structure, spore morphology and ITS sequence, SC2 was identified as Aspergillus niger. Strain SC2 has good cellulose degradation ability, can effectively promote the degradation of straw and be used to prepare corn straw decomposing agent.

Key words: corn straw, corn straw cellulose, cellulose degradation, degradation, enzyme activity, screening, identification

CLC Number:

S182

Yang Na, He Xin, Du Chunmei. Screening and Identification of a Cellulose Degrading Strain[J]. Chinese Agricultural Science Bulletin, 2021, 37(17): 26-31.

Figures/Tables 5

References 33

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菌株编号	透明圈直径(D)/mm	菌落直径(d)/mm	透明圈直径/菌落直径(D/d)
ND9	5.32±0.12	2.81±0.10	1.89 b
ND19	10.26±0.30	3.62±0.20	2.83 a
NC2	8.02±0.37	6.79±0.22	1.18 e
NC4	10.79±0.38	7.68±0.19	1.4 d
SC2	31.80±0.36	21.77±0.27	1.46 c
SC4	55.71±0.51	47.01±0.26	1.19 e

菌株编号	透明圈直径(D)/mm	菌落直径(d)/mm	透明圈直径/菌落直径(D/d)
ND9	5.32±0.12	2.81±0.10	1.89 b
ND19	10.26±0.30	3.62±0.20	2.83 a
NC2	8.02±0.37	6.79±0.22	1.18 e
NC4	10.79±0.38	7.68±0.19	1.4 d
SC2	31.80±0.36	21.77±0.27	1.46 c
SC4	55.71±0.51	47.01±0.26	1.19 e