Comparative Analysis of Chemical Constituents of Polygonatum Cyrtonema Hua of Red and Green Stem Type Based on LC-MS Metabolomics

doi:10.11924/j.issn.1000-6850.casb2020-0407

Abstract

Abstract:

To explore the differences of agronomic characters and medicinal effective components of Polygonatum cyrtonema from different areas of Zhejiang Province, and to provide basic data for the breeding of P. cyrtonema, the agronomic characters of red and green stem P. cyrtonema were firstly compared. Subsequently, the chemical substances of P. cyrtonema rhizome were analyzed by LC-MS and compared with NIST 11 database combined with principal component analysis. The results showed that the difference of plant height, stem diameter, leaf width and leaf area of P. cyrtonema from different provenances were extremely significant; 22 compounds were identified in 12 P. cyrtonema samples, and there were significant differences in VIP values of chemical components and metabolites between the two germplasms. The place of origin and environment of P. cyrtonema not only affect the apparent properties of plants, but also influence the formation and accumulation of its chemical substances. Thus, the agronomic properties and medicinal active components of P. cyrtonema with red stem are better than those with green stem, and the results can provide a theoretical basis for P. cyrtonema breeding and industry promotion in Zhejiang.

Key words: Polygonatum cyrtonema, germplasms, LC-MS, metabolomics, chemical composition

CLC Number:

S184

Jiang Wu, Weng Guohang, Chen Jiadong, Ye Chuansheng, Jiang Xinkai, Tao Zhengming. Comparative Analysis of Chemical Constituents of Polygonatum Cyrtonema Hua of Red and Green Stem Type Based on LC-MS Metabolomics[J]. Chinese Agricultural Science Bulletin, 2021, 37(17): 32-38.

Figures/Tables 8

References 30

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时间/min	流速/(μL/min)	A/%	B/%
0.0	300	100	0
0	300	100	0
1.5	300	80	20
9.5	300	0	100
14.5	300	0	100
14.6	300	100	0
18	300	100	0

时间/min	流速/(μL/min)	A/%	B/%
0.0	300	100	0
0	300	100	0
1.5	300	80	20
9.5	300	0	100
14.5	300	0	100
14.6	300	100	0
18	300	100	0

种质	叶厚/mm	叶长/cm	叶宽/cm	叶面积/cm²	株高/cm	茎粗/mm
G1	0.21±0.01a	18.21±0.21a	5.29±0.35a	63.06±0.73a	108.81±1.44b	9.03±0.02c
G2	0.20±0.01a	17.42±0.42a	5.72±0.23a	62.83±0.52a	108.94±0.57b	8.79±0.14d
G3	0.21±0.01a	18.14±0.16a	5.25±0.19a	62.50±0.79a	109.94±0.43b	8.99±0.04d
R1	0.25±0.01b	19.31±0.65b	6.34±0.04b	75.43±0.05b	98.94±0.63a	7.62±0.22a
R2	0.27±0.01b	19.17±0.68b	6.44±0.19b	74.21±1.16b	98.13±0.39a	7.98±0.08b
R3	0.26±0.01b	19.11±0.29b	6.50±0.40b	74.87±0.19b	98.86±0.68a	7.66±0.04a

种质	叶厚/mm	叶长/cm	叶宽/cm	叶面积/cm²	株高/cm	茎粗/mm
G1	0.21±0.01a	18.21±0.21a	5.29±0.35a	63.06±0.73a	108.81±1.44b	9.03±0.02c
G2	0.20±0.01a	17.42±0.42a	5.72±0.23a	62.83±0.52a	108.94±0.57b	8.79±0.14d
G3	0.21±0.01a	18.14±0.16a	5.25±0.19a	62.50±0.79a	109.94±0.43b	8.99±0.04d
R1	0.25±0.01b	19.31±0.65b	6.34±0.04b	75.43±0.05b	98.94±0.63a	7.62±0.22a
R2	0.27±0.01b	19.17±0.68b	6.44±0.19b	74.21±1.16b	98.13±0.39a	7.98±0.08b
R3	0.26±0.01b	19.11±0.29b	6.50±0.40b	74.87±0.19b	98.86±0.68a	7.66±0.04a

种质	黄精多糖含量/%	最大值/%	最小值/%
G1	8.14±0.08a	8.20	8.05
G2	8.84±0.41a	9.02	8.37
G3	8.72±0.58a	9.36	8.23
R1	11.02±0.70b	11.59	10.23
R2	11.67±1.02b	12.69	10.66
R3	11.14±0.51b	11.56	10.58