Abstract:

To explore the effect of municipal solid waste stacking on the structure, diversity and function of soil bacteria, by using the Illumina high-throughput sequencing technology, we investigated the soil bacterial community structure and function from the municipal solid waste stacking soil and the no waste stacking soil 50 m away from waste stacking soil in Shuangcheng District, Harbin. The results showed that the control group and the treatment group obtained 85435 and 76432 effective sequences, respectively; compared with the control soil, the Chao1 index of bacteria in treatment soil decreased by 14.47% and the Shannon index decreased by 15.01%; the dominant bacterial phyla of two treatment soils were Proteobacteria, Bacteroidetes, Chloroflexi, Acidobacteria, Actinobacteria, Gemmatimonadetes and Cyanobacteria, but the relative abundances were significantly different, in which Chloroflexi increased most by 7.0% and Bacteroidetes decreased most by 12.6%. The relative abundances of Bacillus and Xanthomonas were the highest. Compared with the control treatment, the relative abundance of Bacillus increased by 4.3%. The results of PICRUSt prediction showed that there were 41 KEGG secondary functional groups in the 6 metabolic pathways, of which amino acid metabolism and carbohydrate metabolism were the dominant functions of the two soil samples in the secondary functional groups. The solid waste stacking had a significant impact on the abundance of functional genes. Solid waste stacking has significant negative effect on the abundance and diversity of soil bacteria, as well as a significant effect on the relative abundance of soil bacterial communities and their function, and the study result could provide theoretical data for elucidating the soil quality after solid waste stacking.

Key words: household garbage, soil bacteria, high-throughput sequencing, PICRUSt function prediction