Abstract:

The purpose is to obtain the transcriptome database of Polygonatum sibiricum Red. and explore the genes involved in seed development and dormancy release. The embryos of Polygonatum sibiricum Red. before and after dormancy were used as materials, the transcriptome of the test samples was sequenced by a new generation of high-throughput sequencing method, and the bioinformatics analysis was carried out systematically. A total of 79716 differentially expressed genes were obtained from the samples before and after dormancy release of Polygonatum sibiricum Red. seeds, including 60074 upregulated expression genes and 19642 downregulated expression genes. There were 130284 differentially expressed genes annotated by go function into 56 subclasses of three categories: biological process, molecular function and cell component. The annotated differentially expressed genes were closely related to metabolic process, biological regulation, cell component synthesis and enzyme catalytic activity. The results of KEGG metabolic pathway showed that there were 65038 differentially expressed genes, involving 138 metabolic pathways, mainly involved in carbon metabolism, secondary metabolite biosynthesis and polysaccharide metabolism. Based on the annotation results in KEGG database, 15 metabolic pathways related to the dormancy release of Polygonatum sibiricum Red. embryo were found. In conclusion, during the process of seed development and dormancy release, a large number of different genes including the expression of embryo morphogenesis, polysaccharide decomposition and protein synthesis are expressed and involved in the interaction of multiple metabolic pathways, forming a complex regulatory network of dormancy release.

Key words: Polygonatum sibiricum Red., embryos, high-throughput sequencing method, dormancy, transcriptome, metabolic pathway, differential gene